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Purpureocillium lilacinum strain and application thereof in prevention and treatment of phyllotreta striolata

A technology of lilac violaceum and flea beetle, which is applied in the field of biological pesticides, can solve problems such as no flea beetle, and achieve the effect of not easily producing drug resistance.

Active Publication Date: 2021-03-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fungus is often used to control crop root-knot nematodes, and it is also effective against some insects, but there is no research report on the control of flea beetle

Method used

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  • Purpureocillium lilacinum strain and application thereof in prevention and treatment of phyllotreta striolata
  • Purpureocillium lilacinum strain and application thereof in prevention and treatment of phyllotreta striolata
  • Purpureocillium lilacinum strain and application thereof in prevention and treatment of phyllotreta striolata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026]Example 1: Separation and Screening of PLSC59A3 strains in PC

[0027] The slope species of the test strain was inoculated on the PDA plate, and the spore was harvested after 2-3 weeks, and the spore concentration was measured by 0.05% Tween-80 solution, and the spore concentration was determined, and the suspension was adjusted. To 10 8 Spores / ml concentration, using a dipping method to immerse 10S in a spore suspension of the test, take out the test insect, and then place it in the bottom pad with wet filter paper, replace the heart blade daily And observe the change of the test worm, and the death adults were picked out after the moisturizing culture and other sodes were determined as the disease. 10 adults per processed, repeated 3 times. Mortality and calibration mortality is based on formal calculation:

[0028] Mortality (%) = Death insect / treatment of insect × 100%

[0029] Calibration mortality (%) = (Treatment mortality - control mortality) / (1-control mortali...

Embodiment 2

[0034] Example 2: Identification of PLSC59A3 strains of PCs PLSC59A3

[0035] Example 1 Shot of the resulting Landus Purple PLSC59A3 strain inoculated on the PDA plate, cultured at 27 ± 1 ° C, and observed the growth of colony growth. Such as figure 1 As shown in the early stage of the colonies, the surface of the fluff; colonies were cultured to day 7, the colonies were circular, the central microblumbers, the middle of the surface were white, the edge was lavender, the fluffy, the back is lavender, the diameter is (42.30 ±) 0.07) mm.

[0036] Example 1 Screening the resulting Purple Purus PLSC59A3 strain under optical microscope, such as figure 2 As shown, the bacteria is separated, branched, transparent, width (2.87 ± 0.11) μm; spore is a single cell, elliptical to circular, chain, smooth, colorless to light yellow, 2 μm ~ 4μm wide, branches of irregular branches or branches of bottlett in the sporoids, and a wide range of branches. The round branches consist of a cylindrical...

Embodiment 3

[0038] Example 3: Landercy Purple PLPLSC59A3 strain branch

[0039] Plate culture: Take the preserved raft, scrape a small amount of spore inoculation to PDA plate, and cultured in a 27 ± 1 ° C incubator for 7-14 days, scraping the coloniment surface, spore spore.

[0040] Polyospilia Pure Powder Production: The above-mentioned spore is suspended with 0.05% Tween-80 solution, inoculated into PD seed culture, 27 ± 1 ° C incubator shake flask culture 2 days form seed fluid, seed liquid inoculation On the large rice medium (in the plastic bag or solid fermenter), the amount of inoculation is about 10% (v / m), and there is no 5 weeks of 25 ± 1 ° C, and the spore separator is collected by spore separators, package refrigeration, spare .

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Abstract

The invention relates to a purpureocillium lilacinum strain and application thereof in prevention and treatment of phyllotreta striolata, and belongs to the technical field of biopesticides. The purpureocillium lilacinum PlSC59A3 strain is already preserved in the guangdong microbial culture collection center, the preservation date is 06 November 2020, and the preservation number is GDMCC 61232. The purpureocillium lilacinum PlSC59A3 strain is efficient in prevention and treatment of phyllotreta striolata, is an entomopathogenic fungus, is used as a biopesticide, has the characteristics of nopollution to the environment, difficulty in generation of drug resistance of pests and no toxicity to human and livestock, and is suitable for production requirements of green healthy agricultural products.

Description

Technical field [0001] The present invention relates to a lavender purple purple strain and its application in the prevention and treatment of yellow songs, which belongs to the technical field of biological pesticide. Background technique [0002] The yellow band jump Phyllotreta striolata is a winger, and the leaf adventures, commonly known as dog-cocker, vegetable, jet, earth flea and yellow flea, etc., referred to as jumping, is an important pests of Chinese crops. The larvae and adults of jumping can be a crop crop. The larvae lives in the soil, and the seed roots and buds, as well as the plant roots, resulting in the lack of seedlings and the plants and even the dead; adult food leaves and The stuffed shields form a dense elliptical perforation, bite the young sprouts, causing the bud dry and even death. The adult has a strong jumping ability facilitates migration, resulting in a variety of harm, and can devastate the seeding of vegetables when severe. The yellow song jumps...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30
Inventor 胡琼波陈纬翁群芳袁文静
Owner SOUTH CHINA AGRI UNIV
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