Preparation and application of Purpureocillium lilacinum biological seedling substrate

Abstract

The invention relates to the technical field of agriculture, in particular to preparation and application of a Purpureocillium lilacinum biological seedling substrate. A functional flora taking a purpureocillium lilacinum strain Pl36-1-1 as a main microorganism is formed in a substrate, so that the healthy growth of plants is protected from a seedling raising stage, and the potential of the microorganism is comprehensively exerted; and the prevention and control effect of microbial products on vegetable soil spread diseases is improved. The method comprises the following steps: S1, adding 10-20 parts of cane sugar, 1-6 parts of trace elements and 15-30 parts of rice husks into 100-150 parts of agricultural wastes, performing uniform stirring, performing fermenting, performing decomposing, performing sieving, adding perlite and vermiculite, and performing uniformly mixing to obtain a primary fermentation product; and S2, mixing 10-20 parts of cane sugar into the primary fermentation product, performing uniform stirring, inoculating Purpureocillium lilacinum Pl36-1-1 strain conidia powder, and performing uniform mixing to obtain the Purpureocillium lilacinum seedling culture substrate.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to the preparation and application of a biological seedling-raising substrate of P. lilacinium. Background technique [0002] Purpureocillium lilacinum has become the most studied nematode biocontrol factor because of its popular contact, safety and non-polluting properties, and the development of an environment-friendly society. It is an important natural enemy of various plant nematodes such as cyst nematodes, heteroderma nematodes, golden nematodes, piercing nematodes and pearl nematodes. Many years of research by Davide et al. have shown that the P. lilacensis inoculum can control potato golden nematode (Globoclerara restrochiensis), and promote the increase of potato yield, and its increase is 20.3%-36.5%. The relative reduction in the number of 66%-77% increases the yield of okra by 45%-54%. Maheswari-Tu research results show that: the mixed use of P. lilacinus and Paste...

AI Technical Summary

Problems solved by technology

However, because there are few varieties of high-quality, high-yield and disease-resistant vegetables in the production of facility vegetables, and the breeding cycle of disease-resistant varieties is long, so agricultural measures such as grafting, chemical control and biological control are often used to control fusarium wilt and root knot disease. nematode disease

Grafting cultivation and soil chemical treatment can effectively prevent and control the occurrence of vegetable wilt and root-knot nematode. The occurrence of root-knot nematodes is increasing year by year; the cost of chemical agent control is high and labor-intensive, and long-term use can easily cause pathogenic bacteria to develop resistance, resulting in a decline in the control effect, and also causes pesticide residues and air pollution; biological control is environmentally friendly and low-risk. However, the potential has not yet been fully utilized. Some biocontrol bacteria with high control effects on soil-borne diseases screened under experimental conditions are greatly affected by the environment in the field, often with low colonization numbers, and have obvious disease control effects. Weakening etc.

Images