Plant growth substance

Abstract

The invention discloses a plant growth substance, which is prepared from the following components in percentage by weight: 30 to 40 percent of compound microbial agent, 15 to 20 percent of dung beetle extract, 30 to 40 percent of yeast liquid and 20 to 30 percent of animal claw shell amino acid aqueous solution, the compound microbial agent is prepared from the following components in percentage by weight: 20 to 35 percent of bacillus mucilaginosus, 20 to 35 percent of bacillus megatherium, 20 to 30 percent of photosynthetic bacteria, 15 to 25 percent of azotobacter chroococcum and 15 to 25 percent of trichoderma harzianum. According to the present invention, the plant resistance can be effectively improved, and the plant diseases such as powdery mildew, damping off, root rot, gummy stem blight, sclerotiniose, verticillium wilt, bacterial wilt, fruit rot, downy mildew, virus disease, root-knot nematode and the like caused by fungi, bacteria and viruses can be inhibited; the use of chemical fertilizers and pesticides can be obviously reduced, the ecological environment is improved, the crop yield is increased, and the planting cost is reduced; the problems of pesticide residue, pesticide harm and high heavy metal residue are thoroughly solved; original quality and taste of crops are restored, and agricultural product quality is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture and relates to a plant growth promoter. Background Art

[0002] In order to increase production, agriculture now uses a large amount of fertilizers and pesticides, which has led to a decline in soil quality and has caused damage to the natural ecology. Pesticide residues and heavy metal residues in agricultural products exceed the standard, seriously affecting food safety and human health. At the same time, it has laid great difficulties and huge costs for future governance. The output has increased, but the quality has declined. The large amount of pesticide and fertilizer damage and heavy metal residues or excessive amounts in agricultural products have caused serious damage to people's food safety and health. This fully shows that modern agriculture must respect nature and protect the ecology; while pursuing production and reducing planting costs, it should also improve the quality of agricultural products, pay attention to food safety and health, and benefit future generations and mankind. Summary of the invention

[0003] The technical problem to be solved by the present invention is that: now agriculture uses a large amount of chemical fertilizers and pesticides, soil quality has obviously declined, the ecological environment has been seriously damaged; pesticide residues and heavy metal residues in agricultural products exceed the standard, seriously affecting food safety and human health. In order to overcome the shortcomings of the existing technology, a plant growth promoter is provided. The invention can effectively improve the plant's own resistance and inhibit the occurrence of diseases; reduce the use of chemical fertilizers and pesticides, improve the ecological environment; increase crop yields and reduce planting costs; decompose agricultural residues and agricultural harms, and improve the quality of agricultural products.

[0004] In order to solve the above technical problems, a plant growth promoter is provided, which comprises, by weight percentage:

[0005] Composite microbial agent 30-40%, dung beetle extract 15-20%, yeast liquid 30-40%, animal claw shell amino acid aqueous solution 20-30%;

[0006] The composite microbial agent comprises, by weight percentage:

[0007] Bacillus subtilis 20-35%, Bacillus megaterium 20-35%, photosynthetic bacteria 20-30%, round brown nitrogen-fixing bacteria 15-25%, Trichoderma harzianum 15-25%.

[0008] The technical solution further defined in the present invention is:

[0009] The preparation method of the dung beetle extract comprises the following specific steps:

[0010] Step 1: Spread 240 mesh zeolite powder in a light-proof inoculation box, put dung beetles in at night, and add induced odor bait and release inducing gas to make them excited to forage and release enzymes to obtain bacterial enzyme soil;

[0011] Step 2: The next day, take the bacterial enzyme soil and add carbon source, nitrogen source, inorganic salt and trace elements to prepare a culture medium;

[0012] Step 3: Add the culture medium into the fermentation tank, control the temperature to 35-38°C, the pH value to 6-8, provide oxygen supply, and inoculate the dung beetle enzyme strain;

[0013] Step 4: The dung beetle enzyme strain grows and metabolizes in the culture medium for 24-30 hours to produce dung beetle enzyme to obtain a fermentation liquid;

[0014] Step 5: Collect the fermentation liquid and separate the bacteria from the fermentation liquid by centrifugation;

[0015] Step 6: Then carry out precipitation, filtration, concentration and chromatography to obtain dung beetle enzyme.

[0016] The technical solution further defined in the present invention is:

[0017] The method for preparing the animal claw shell amino acid aqueous solution comprises the following specific steps:

[0018] Step 1: After cleaning and drying the claws of the animals, crush them into 80-mesh powder and put them into an alkali-resistant container;

[0019] Step 2: Mix 30-50% by weight of edible hydrochloric acid and animal claw shell powder in a ratio of 1:4, and heat to a constant temperature of 60-70° C. until completely decomposed;

[0020] Step 3: Cool to room temperature, gradually add sodium bicarbonate to neutralize to a pH value between 6 and 8, and obtain an animal claw shell amino acid aqueous solution.

[0021] Furthermore, the animal claw shell amino acid aqueous solution comprises, by weight percentage:

[0022] Phenylalanine 7-9%, methionine 8-12%, tryptophan 3-5%, lysine 40-50%, leucine 3-5%, threonine 6-10%, aspartic acid 8-10%, glutamic acid 8-10%, arginine 1-2%, histidine 1-1.5%, alanine 0.5-1%, calcium aspartate 3-5%.

[0023] Furthermore, in step 2, the culture medium comprises, by weight percentage:

[0024] Carbon source 30-40%, nitrogen source 30-35%, inorganic salt 18-23% and trace elements 5-12%.

[0025] Furthermore, in step 1, the animal claw shell includes one or more claw shells of pigs, cattle, and sheep.

[0026] Furthermore, in step one, the odor-inducing bait is one or a combination of stinky amaranth, stinky tofu and fresh cow dung.

[0027] The beneficial effects of the present invention are:

[0028] The present invention proposes a plant growth promoter, which can effectively improve the plant's own resistance, inhibit the occurrence of various plant diseases caused by bacteria, fungi, viruses and root-knot nematodes, eliminate pesticide and fertilizer damage, completely solve the problem of high residues of heavy metals such as lead, arsenic, cadmium, mercury, and benzopyrene, and restore the original taste and quality of crops.

[0029] The present invention can effectively promote the gathering, collection, absorption and transformation of carbon dioxide in the air by plants, and fully utilize air, water and sunlight to synthesize nutrients required for plant growth, thereby reducing the use of chemical fertilizers. The growth, fruit setting rate, yield and quality of crops are better than those of chemical agriculture.

[0030] The dung beetle extract of the present invention is an extremely active animal enzyme, which can quickly decompose fertilizer, pesticide residues and harmful substances thereof, and completely solve the problem of high residues of heavy metals such as nitrite, lead, arsenic, cadmium, mercury, benzopyrene, etc.; at the same time, it activates indigenous bacteria to form beneficial bacteria, decomposes harmful substances such as chemical antibiotics and hormones, plays an effect of improving the soil, and can also realize the repeated planting of multiple plants.

[0031] The animal claw shell amino acid aqueous solution of the present invention can not only provide nutrition to plants, but also promote plants to improve their own resistance, inhibit various diseases caused by bacteria, fungi and viruses in plants, effectively promote the development of plant root systems, lush leaves and strong stems, and greatly reduce the use of chemical pesticides. DETAILED DESCRIPTION

[0032] The present invention is further described below through specific embodiments, wherein:

[0033] Example 1

[0034] This embodiment provides a plant growth promoter, which comprises, by weight percentage:

[0035] Composite microbial agent 30%, dung beetle extract 20%, yeast liquid 30%, animal claw shell amino acid aqueous solution 20%;

[0036] The composite microbial agent comprises, by weight percentage:

[0037] Bacillus subtilis 20%, Bacillus megaterium 20%, photosynthetic bacteria 20%, round brown nitrogen-fixing bacteria 15%, Trichoderma harzianum 25%.

[0038] The technical solution further defined in the present invention is:

[0039] The preparation method of the dung beetle extract comprises the following specific steps:

[0040] Step 1: Spread 240 mesh zeolite powder in a light-proof inoculation box, put dung beetles in at night, and add induced odor bait and release inducing gas to make them excited to forage and release enzymes to obtain bacterial enzyme soil;

[0041] Step 2: The next day, take the bacterial enzyme soil and add carbon source, nitrogen source, inorganic salt and trace elements to prepare a culture medium;

[0042] Step 3: Add the culture medium into the fermentation tank, control the temperature to 35°C and the pH value to between 6, provide oxygen supply, and inoculate the dung beetle enzyme strain;

[0043] Step 4: The dung beetle enzyme strain grows and metabolizes in the culture medium for 24 hours to produce dung beetle enzyme to obtain a fermentation liquid;

[0044] Step 5: Collect the fermentation liquid and separate the bacteria from the fermentation liquid by centrifugation;

[0045] Step 6: Then carry out precipitation, filtration, concentration and chromatography to obtain dung beetle enzyme.

[0046] The technical solution further defined in the present invention is:

[0047] The method for preparing the animal claw shell amino acid aqueous solution comprises the following specific steps:

[0048] Step 1: After cleaning and drying the claws of the animals, crush them into 80-mesh powder and put them into an alkali-resistant container;

[0049] Step 2: Mix 30% by mass of edible hydrochloric acid and animal claw shell powder in a ratio of 1:4, and heat to a constant temperature of 60°C until completely decomposed;

[0050] Step 3: Cool to room temperature, gradually add sodium bicarbonate to neutralize to a pH value of 6, and obtain an animal claw shell amino acid aqueous solution.

[0051] Furthermore, the animal claw shell amino acid aqueous solution comprises, by weight percentage:

[0052] Phenylalanine 9%, methionine 12%, tryptophan 3%, lysine 40%, leucine 3%, threonine 10%, aspartic acid 8%, glutamic acid 8%, arginine 2%, histidine 1%, alanine 1%, calcium aspartate 3%.

[0053] Furthermore, in step 2, the culture medium comprises, by weight percentage:

[0054] Carbon source 40%, nitrogen source 30%, inorganic salt 23% and trace elements 7%.

[0055] Furthermore, in step 1, the animal claw shell includes: a pig claw shell.

[0056] Furthermore, in step one, the odor-inducing bait is stinky amaranth.

[0057] The plant growth promoter of the embodiment was diluted 20 times and used for foliar spraying in the tillering stage of rice, which can effectively increase the number of tillering plants and make the growth uniform. It was used for foliar spraying from the initial heading stage to the filling stage of rice to increase the fullness rate of rice ears, and there was no blue and white. Compared with the control group, the use of fertilizers and pesticides in the whole growth cycle was reduced, and the yield was increased. It was also effective for other crops (wheat, corn, soybean, etc.).

[0058] Diluting the plant growth promoter of the embodiment and spraying it on berries and vegetables in the middle and late stages is beneficial to increasing the amino acid content and can significantly improve the quality of fruits and vegetables. The sugar content and yield of berries are increased, and the yield of leafy vegetables is greatly increased.

[0059] Example 2

[0060] This embodiment provides a plant growth promoter, which comprises, by weight percentage:

[0061] Composite microbial agent 40%, dung beetle extract 15%, yeast liquid 30%, animal claw shell amino acid aqueous solution 15%;

[0062] The composite microbial agent comprises, by weight percentage:

[0063] Bacillus subtilis 20%, Bacillus megaterium 30%, photosynthetic bacteria 20%, round brown nitrogen-fixing bacteria 15%, Trichoderma harzianum 15%.

[0064] The technical solution further defined in the present invention is:

[0065] The preparation method of the dung beetle extract comprises the following specific steps:

[0066] Step 1: Spread 240 mesh zeolite powder in a light-proof inoculation box, put dung beetles in at night, and add induced odor bait and release inducing gas to make them excited to forage and release enzymes to obtain bacterial enzyme soil;

[0067] Step 2: The next day, take the bacterial enzyme soil and add carbon source, nitrogen source, inorganic salt and trace elements to prepare a culture medium;

[0068] Step 3: Add the culture medium into the fermentation tank, control the temperature to 38°C and the pH value to 8, provide oxygen supply, and inoculate the dung beetle enzyme strain;

[0069] Step 4: The dung beetle enzyme strain grows and metabolizes in the culture medium for 30 hours to produce dung beetle enzyme to obtain a fermentation liquid;

[0070] Step 5: Collect the fermentation liquid and separate the bacteria from the fermentation liquid by centrifugation;

[0071] Step 6: Then carry out precipitation, filtration, concentration and chromatography to obtain dung beetle enzyme.

[0072] The technical solution further defined in the present invention is:

[0073] The method for preparing the animal claw shell amino acid aqueous solution comprises the following specific steps:

[0074] Step 1: After cleaning and drying the claws of the animals, crush them into 80-mesh powder and put them into an alkali-resistant container;

[0075] Step 2: Mix 50% by mass of edible hydrochloric acid and animal claw shell powder in a ratio of 1:4, and heat to a constant temperature of 70°C until completely decomposed;

[0076] Step 3: Cool to room temperature, gradually add sodium bicarbonate to neutralize to a pH of 8, and obtain an animal claw shell amino acid aqueous solution.

[0077] Furthermore, the animal claw shell amino acid aqueous solution comprises, by weight percentage:

[0078] Phenylalanine 7%, methionine 8%, tryptophan 5%, lysine 43.5%, leucine 5%, threonine 6%, aspartic acid 10%, glutamic acid 8%, arginine 1%, histidine 1%, alanine 0.5%, calcium aspartate 5%.

[0079] Furthermore, in step 2, the culture medium comprises, by weight percentage:

[0080] Carbon source 35%, nitrogen source 35%, inorganic salt 18% and trace elements 12%.

[0081] Furthermore, in step 1, the animal claw shell includes: a cow claw shell.

[0082] Furthermore, in step one, the odor-inducing bait is stinky tofu.

[0083] The plant growth promoter of the embodiment was diluted 30 times and used for foliar spraying in the tillering stage of rice, which can effectively increase the number of tillering plants and make the growth uniform. It was used for foliar spraying from the initial heading stage to the filling stage of rice to increase the fullness rate of rice ears, and there was no blue and white. Compared with the control group, the use of fertilizers and pesticides in the whole growth cycle was reduced, and the yield was increased. It was also effective for other crops (wheat, corn, soybean, etc.).

[0084] Diluting the plant growth promoter of the embodiment and spraying it on berries and vegetables in the middle and late stages is beneficial to increasing the amino acid content and can significantly improve the quality of fruits and vegetables. The sugar content and yield of berries are increased, and the yield of leafy vegetables is greatly increased.

[0085] Example 3

[0086] This embodiment provides a plant growth promoter, which comprises, by weight percentage:

[0087] Composite microbial agent 30%, dung beetle extract 15%, yeast liquid 30%, animal claw shell amino acid aqueous solution 25%;

[0088] The composite microbial agent comprises, by weight percentage:

[0089] Bacillus subtilis 20%, Bacillus megaterium 20%, photosynthetic bacteria 20%, round brown nitrogen-fixing bacteria 25%, Trichoderma harzianum 15%.

[0090] The technical solution further defined in the present invention is:

[0091] The preparation method of the dung beetle extract comprises the following specific steps:

[0092] Step 1: Spread 240 mesh zeolite powder in a light-proof inoculation box, put dung beetles in at night, and add induced odor bait and release inducing gas to make them excited to forage and release enzymes to obtain bacterial enzyme soil;

[0093] Step 2: The next day, take the bacterial enzyme soil and add carbon source, nitrogen source, inorganic salt and trace elements to prepare a culture medium;

[0094] Step 3: Add the culture medium into the fermentation tank, control the temperature to 36°C and the pH value to 7, provide oxygen supply, and inoculate the dung beetle enzyme strain;

[0095] Step 4: the dung beetle enzyme strain grows and metabolizes in the culture medium for 27 hours to produce dung beetle enzyme to obtain a fermentation liquid;

[0096] Step 5: Collect the fermentation liquid and separate the bacteria from the fermentation liquid by centrifugation;

[0097] Step 6: Then carry out precipitation, filtration, concentration and chromatography to obtain dung beetle enzyme.

[0098] The technical solution further defined in the present invention is:

[0099] The method for preparing the animal claw shell amino acid aqueous solution comprises the following specific steps:

[0100] Step 1: After cleaning and drying the claws of the animals, crush them into 80-mesh powder and put them into an alkali-resistant container;

[0101] Step 2: Mix 40% by weight of edible hydrochloric acid and animal claw shell powder in a ratio of 1:4, and heat to a constant temperature of 65°C until completely decomposed;

[0102] Step 3: Cool to room temperature, gradually add sodium bicarbonate to neutralize to pH 7, and obtain an animal claw shell amino acid aqueous solution.

[0103] Furthermore, the animal claw shell amino acid aqueous solution comprises, by weight percentage:

[0104] Phenylalanine 7%, methionine 8%, tryptophan 3%, lysine 48%, leucine 3%, threonine 6%, aspartic acid 8%, glutamic acid 10%, arginine 1%, histidine 1.2%, alanine 0.8%, calcium aspartate 4%.

[0105] Furthermore, in step 2, the culture medium comprises, by weight percentage:

[0106] Carbon source 40%, nitrogen source 35%, inorganic salt 2% and trace elements 5%.

[0107] Furthermore, in step 1, the animal claw shell includes: sheep claw shell.

[0108] Furthermore, in step one, the odor-inducing bait is fresh cow dung.

[0109] The plant growth promoter of the embodiment was diluted 25 times and used for foliar spraying in the tillering stage of rice, which can effectively increase the number of tillering plants and make the growth uniform. It was used for foliar spraying from the initial heading stage to the filling stage of rice to increase the fullness rate of rice ears, and there was no blue and white. Compared with the control group, the use of fertilizers and pesticides in the whole growth cycle was reduced, and the yield was increased. It was also effective for other crops (wheat, corn, soybean, etc.).

[0110] Diluting the plant growth promoter of the embodiment and spraying it on berries and vegetables in the middle and late stages is beneficial to increasing the amino acid content and can significantly improve the quality of fruits and vegetables. The sugar content and yield of berries are increased, and the yield of leafy vegetables is greatly increased.

[0111] The plant growth promoters of Examples 1, 2 and 3 were respectively used for foliar spraying at the tillering stage of rice and foliar spraying from the heading stage to the grain filling stage of rice, and the effects of use were as follows:

[0112]

[0113]

[0114] The plant growth promoters of Examples 1, 2 and 3 are respectively used for foliar spraying of vegetables and fruits in the middle and late stages, which is beneficial to increase the amino acid content, significantly increase the yield and improve the quality of fruits and vegetables. The sugar content of berries is increased by 1.5-5%, the yield is increased by 10-30%, and the yield of leafy vegetables is increased by more than 30%.

[0115] The above embodiments are only for illustrating the technical idea of ​​the present invention, and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution in accordance with the technical idea proposed by the present invention shall fall within the protection scope of the present invention.

Claims

1. A plant growth promoter, characterized in that In terms of weight percentage, it includes: Composite microbial agent 30-40%, dung beetle extract 15-20%, yeast liquid 30-40%, animal claw shell amino acid aqueous solution 20-30%; The composite microbial agent comprises, by weight percentage: Bacillus subtilis 20-35%, Bacillus megaterium 20-35%, photosynthetic bacteria 20-30%, round brown nitrogen-fixing bacteria 15-25%, Trichoderma harzianum 15-25%.

2. The plant growth promoter according to claim 1, characterized in that The preparation method of the dung beetle extract comprises the following specific steps: Step 1: Spread 240 mesh zeolite powder in a light-proof inoculation box, put dung beetles in at night, and add induced odor bait and release inducing gas to make them excited to forage and release enzymes to obtain bacterial enzyme soil; Step 2: The next day, take the bacterial enzyme soil and add carbon source, nitrogen source, inorganic salt and trace elements to prepare a culture medium; Step 3: Add the culture medium into the fermentation tank, control the temperature to 35-38°C, the pH value to 6-8, provide oxygen supply, and inoculate the dung beetle enzyme strain; Step 4: The dung beetle enzyme strain grows and metabolizes in the culture medium for 24-30 hours to produce dung beetle enzyme to obtain a fermentation liquid; Step 5: Collect the fermentation liquid and separate the bacteria from the fermentation liquid by centrifugation; Step 6: Then carry out precipitation, filtration, concentration and chromatography to obtain dung beetle enzyme.

3. The plant growth promoter according to claim 1, characterized in that The method for preparing the animal claw shell amino acid aqueous solution comprises the following specific steps: Step 1: After cleaning and drying the claws of the animals, crush them into 80-mesh powder and put them into an alkali-resistant container; Step 2: Mix 30-50% by weight of edible hydrochloric acid and animal claw shell powder in a ratio of 1:4, and heat to a constant temperature of 60-70° C. until completely decomposed; Step 3: Cool to room temperature, gradually add sodium bicarbonate to neutralize to a pH value between 6 and 8, and obtain an animal claw shell amino acid aqueous solution.

4. The plant growth promoter according to claim 1, characterized in that The animal claw shell amino acid aqueous solution comprises, by weight percentage: Phenylalanine 7-9%, methionine 8-12%, tryptophan 3-5%, lysine 40-50%, leucine 3-5%, threonine 6-10%, aspartic acid 8-10%, glutamic acid 8-10%, arginine 1-2%, histidine 1-1.5%, alanine 0.5-1%, calcium aspartate 2-3%.

5. The plant growth promoter according to claim 2, characterized in that In the step 2, the culture medium comprises, by weight percentage: Carbon source 30-40%, nitrogen source 30-35%, inorganic salt 18-23% and trace elements 5-12%.

6. The plant growth promoter according to claim 3, characterized in that In step 1, the animal claw shell includes one or more claw shells of pigs, cattle, and sheep.

7. The plant growth promoter according to claim 2, characterized in that In the step 1, the odor inducing bait is one or a combination of stinky amaranth, stinky tofu and fresh cow dung.

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