Complex lively bacterial preparation as well as water soluble fertilizer and application thereof

A technology of compound bacterial agent and water-soluble fertilizer, which is applied in the direction of application, bacteria, fungi, etc., can solve the problems of unreasonable nutrient formula and high price of water-soluble fertilizer, and achieve the effects of avoiding adverse effects, good antagonism effect, and reducing cultivation cost

Active Publication Date: 2015-11-11
北京沃土天地生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the defects of high price of water-soluble fertilizer and unreasonable nutrient form...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a kind of composite bacterial agent, is made by following method:

[0031] 1) Mix the seed liquids with an OD value of 3.8 Bacillus subtilis, 3.5 saccharomyces, 3.6 glial bacillus and 3.6 Bacillus licheniformis in a volume ratio of 1:3:4:2 to obtain a mixed seed liquid;

[0032]2) Carry out high-density mixed fermentation culture in the by-products of yeast fermentation with the mixed seed liquid according to the inoculum size of 5%, aerate at intervals within 0 to 24 hours at the beginning, keep fermentation under aerobic conditions, and then cultivate During the process, adjust the fermentation dissolved oxygen to 5-15%, the stirring speed is 150-200r / min, the stirring interval is 1-3 hours, and stir for 1-3 minutes until the total number of viable bacteria is 2 billion / mL, stop the cultivation, and obtain the obtained The above-mentioned composite bacterial agent.

Embodiment 2

[0034] The present embodiment provides a kind of composite bacterial agent, is made by following method:

[0035] 1) Mix the seed liquids of Bacillus subtilis with an OD value of 3.5, 3.0 yeast, 3.5 colloidal bacteria and 3.5 Bacillus licheniformis in a volume ratio of 1:2:2:3 to obtain a mixed seed liquid;

[0036] 2) The mixed seed solution is carried out in a high-density mixed fermentation culture in the by-products of yeast fermentation according to the inoculum size of 20%. In the initial 0-24 hours, aeration is performed at intervals, and fermentation is maintained under aerobic conditions. During the process, adjust the fermentation dissolved oxygen to 5-15%, the stirring speed is 150-200r / min, the stirring interval is 1-3 hours, and stir for 1-3 minutes until the total number of viable bacteria is 1 billion / mL, stop the cultivation, and obtain the obtained The above-mentioned composite bacterial agent.

Embodiment 3

[0038] The present embodiment provides a kind of composite bacterial agent, is made by following method:

[0039] 1) Mix the seed solutions of Bacillus subtilis with an OD value of 4.0, 4.0 yeast, 4.0 colloid and 4.0 Bacillus licheniformis in a volume ratio of 1:1:1:1 to obtain a mixed seed solution;

[0040] 2) The mixed seed solution is carried out in a high-density mixed fermentation culture in the by-products of yeast fermentation according to the inoculum size of 10%. In the initial 0-24 hours, aeration is performed at intervals, and fermentation is maintained under aerobic conditions. During the process, adjust the fermentation dissolved oxygen to 5-15%, the stirring speed is 150-200r / min, the stirring interval is 1-3 hours, and stir for 1-3 minutes until the total number of viable bacteria is 10 billion / mL, stop the cultivation, and obtain the obtained The above-mentioned composite bacterial agent.

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Abstract

The invention relates to a complex lively bacterial preparation which is prepared through the following method: (1) mixing the seed solutions of bacillus subtilis with an OD value of 3.5 to 4.0, saccharomycetes with an OD value of 3.0 to 4.0, bacillus mucilaginosus with an OD value of 3.5 to 4.0 and bacillus licheniformis with an OD value of 3.5 to 4.0 according to the volume ratio of (1 to 5):(1 to 5):(1 to 5):(1 to 5), so as to obtain a mixed seed solution;(2) carrying out high density mixed fermentation cultivation on the mixed seed solution in a by-product produced during yeast fermentation, and not stopping until the total of viable counts is larger than 1 billion/mL, so as to obtain the complex lively bacterial preparation. The complex lively bacterial preparation provided by the invention can be mixed with nutrient elements such as nitrogen and phosphorus to prepare a microorganism water soluble fertilizer, and the water soluble fertilizer prepared from the complex lively bacterial preparation is comprehensive in nutrition and can be easily absorbed by plants, the preparation raw materials of the fertilizer are cheap and easy to obtain, and the microorganism therein can inhibit the growth of pathogenic bacteria, promote the growth of crops and induce the plants to generate resistance, and takes the effects of prophylaxis and growth promoting on the crops.

Description

technical field [0001] The invention belongs to the field of plant fertilizers, and in particular relates to a composite bacterial agent and a water-soluble fertilizer containing the composite bacterial agent. Background technique [0002] The definition of water-soluble fertilizer (WaterSolubleFertilizer, WSF for short, water-soluble fertilizer) in my country's agricultural industry standard is: water-soluble fertilizer is dissolved or diluted in water, used for irrigation and fertilization, foliar fertilization, soilless cultivation, soaking seeds and dipping roots, etc. liquid or solid fertilizers. my country is a large agricultural country with a shortage of water resources, and water-saving irrigation is a practical and effective way to save water resources and agricultural costs. Because water-soluble fertilizers can be completely dissolved in water, they can be combined with farmland irrigation to form an integration of water and fertilizer, which saves production cos...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05G3/00C12R1/125C12R1/10C12R1/07
Inventor 任莉靳晓藜王凯彭生平
Owner 北京沃土天地生物科技股份有限公司
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