Complex lively bacterial preparation as well as water soluble fertilizer and application thereof
A technology of compound bacterial agent and water-soluble fertilizer, which is applied in the direction of application, bacteria, fungi, etc., can solve the problems of unreasonable nutrient formula and high price of water-soluble fertilizer, and achieve the effects of avoiding adverse effects, good antagonism effect, and reducing cultivation cost
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Embodiment 1
[0030] The present embodiment provides a kind of composite bacterial agent, is made by following method:
[0031] 1) Mix the seed liquids with an OD value of 3.8 Bacillus subtilis, 3.5 saccharomyces, 3.6 glial bacillus and 3.6 Bacillus licheniformis in a volume ratio of 1:3:4:2 to obtain a mixed seed liquid;
[0032]2) Carry out high-density mixed fermentation culture in the by-products of yeast fermentation with the mixed seed liquid according to the inoculum size of 5%, aerate at intervals within 0 to 24 hours at the beginning, keep fermentation under aerobic conditions, and then cultivate During the process, adjust the fermentation dissolved oxygen to 5-15%, the stirring speed is 150-200r / min, the stirring interval is 1-3 hours, and stir for 1-3 minutes until the total number of viable bacteria is 2 billion / mL, stop the cultivation, and obtain the obtained The above-mentioned composite bacterial agent.
Embodiment 2
[0034] The present embodiment provides a kind of composite bacterial agent, is made by following method:
[0035] 1) Mix the seed liquids of Bacillus subtilis with an OD value of 3.5, 3.0 yeast, 3.5 colloidal bacteria and 3.5 Bacillus licheniformis in a volume ratio of 1:2:2:3 to obtain a mixed seed liquid;
[0036] 2) The mixed seed solution is carried out in a high-density mixed fermentation culture in the by-products of yeast fermentation according to the inoculum size of 20%. In the initial 0-24 hours, aeration is performed at intervals, and fermentation is maintained under aerobic conditions. During the process, adjust the fermentation dissolved oxygen to 5-15%, the stirring speed is 150-200r / min, the stirring interval is 1-3 hours, and stir for 1-3 minutes until the total number of viable bacteria is 1 billion / mL, stop the cultivation, and obtain the obtained The above-mentioned composite bacterial agent.
Embodiment 3
[0038] The present embodiment provides a kind of composite bacterial agent, is made by following method:
[0039] 1) Mix the seed solutions of Bacillus subtilis with an OD value of 4.0, 4.0 yeast, 4.0 colloid and 4.0 Bacillus licheniformis in a volume ratio of 1:1:1:1 to obtain a mixed seed solution;
[0040] 2) The mixed seed solution is carried out in a high-density mixed fermentation culture in the by-products of yeast fermentation according to the inoculum size of 10%. In the initial 0-24 hours, aeration is performed at intervals, and fermentation is maintained under aerobic conditions. During the process, adjust the fermentation dissolved oxygen to 5-15%, the stirring speed is 150-200r / min, the stirring interval is 1-3 hours, and stir for 1-3 minutes until the total number of viable bacteria is 10 billion / mL, stop the cultivation, and obtain the obtained The above-mentioned composite bacterial agent.
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