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Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof

A technology of composite biological bacteria and microbial bacteria, which is applied in application, fertilizer mixture, fertilization equipment, etc., can solve the problems of short shelf life of liquid bacterial fertilizer, single effect of bacterial fertilizer, and easy inactivation of bacteria, so as to improve the growth environment of crops, Easy to absorb by roots and inhibit life activities

Active Publication Date: 2014-02-05
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are more and more compound microbial fertilizers on the market, but most of them have a single effect and high price, and most of the liquid bacterial fertilizers have a short shelf life and the bacteria are easily inactivated

Method used

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  • Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof
  • Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof
  • Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Preparation of medium

[0057] No. 1 medium: by mass percentage, 0.8%-1.2% peptone, 0.5%-1% yeast powder, 0.3%-1.0% NaCl, the rest is water, pH7.2-7.4.

[0058] No. 2 medium: by mass percentage, 1%-2% mannitol or sucrose, 0.1‰-0.12‰CaSO 4 2H 2 O, 0.18‰-0.25‰K 2 HPO 4 , 0.5%-0.6%CaCO 3 , 0.15‰-0.2‰MgSO 4 ·7H 2 O, 0.2‰-0.25‰NaCl, 1.4%-2% agar, the rest is water.

[0059] Medium 3: by mass percentage, 1.0%-1.2% glucose, 0.5%-0.6%K 2 HPO 4 ·3H 2 O, 0.3%-0.5%KH 2 PO 4 , 0.1%-0.2% urea, 0.4%-0.6% yeast powder, 0.35%-0.45‰MgSO 4 ·7H 2 O, the rest is water, pH7.0-7.2.

[0060] No. 4 medium: by mass percentage, 1% sucrose, 0.5% K 2 HPO 4 ·3H 2 O, 0.5‰yeast powder, 0.02‰FeCl 3 ·6H 2 O, 0.4‰CaCO 3 , 0.2‰MgSO 4 ·7H 2 O, the rest is water, pH7.0.

[0061] (2) Slope activation

[0062] Bacillus thuringiensis, Bacillus megaterium, and Bacillus amyloliquefaciens were inoculated on the No. 1 plate medium for activation culture, and Bacillus colloidus was stre...

Embodiment 2

[0078] (1) Preparation of medium

[0079] No. 1 medium: by mass percentage, 1% peptone, 0.5% yeast powder, 0.3% NaCl, the rest is water, pH7.2;

[0080] No. 2 medium: by mass percentage, 1% mannitol, 0.1‰CaSO 4 2H 2 O, 0.2‰K 2 HPO 4 , 0.5%CaCO3 , 0.2‰MgSO 4 ·7H 2 O, 0.2‰NaCl, 1.4% agar, the rest is water;

[0081] Medium 3: by mass percentage, 1.0% glucose, 0.6% K 2 HPO 4 , 0.3%KH 2 PO 4 , 0.15% urea, 0.5% yeast powder, 0.4‰MgSO 4 ·7H 2 O, the rest is water, pH7.2

[0082] No. 4 medium: by mass percentage, 1% sucrose, 0.5% K 2 HPO 4 ·3H 2 O, 0.5‰yeast powder, 0.02‰FeCl 3 ·6H 2 O, 0.4‰CaCO 3 , 0.25‰MgSO 4 ·7H 2 O, the rest is water, pH7.0,

[0083] (2) Slope activation

[0084] Activate the bacterial strains stored at 4°C, then streak and inoculate Bacillus thuringiensis, Bacillus megaterium, and Bacillus amyloliquefaciens on the No. 1 plate medium, and culture at 30°C-37°C for 1 day; Line inoculation in No. 2 medium, culture at 25°C-30°C for 1-2d.

[00...

Embodiment 3

[0107] According to the method described in (1)-(4) in Example 2, prepare the fermentation broth of the four bacteria described in Example 2, mix the collected four fermentation broths in equal volumes, centrifuge at 4°C, 3000rpm, 8min, and discard Supernatant, collect the bacteria, resuspend the bacteria with 1 / 8 (V / V) sterile water of the total fermentation broth, add kaolin with 15% (m / V) of the total fermentation broth, and adsorb on a magnetic stirrer for 30min , let stand for 10 minutes; add compound nutrients and additives, and make the volume equal to the volume of the mixed fermentation broth before centrifugation. Fill and preserve according to the method described in (8) of Example 1, and at the same time, use the product without the addition of bacterial cell protection agent and additives as a control. Regularly measure the total number of viable bacteria.

[0108] The weight part array of described compound nutrient becomes: Potassium dihydrogen phosphate 50, ED...

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Abstract

The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9> / mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.

Description

technical field [0001] The invention belongs to the field of compound microbial bacterial fertilizer, and in particular relates to a liquid compound biological fertilizer with a long shelf life and a preparation method and application thereof. Background technique [0002] Ecological civilization is a contemporary trend, and the development of agriculture is closely related to the ecological environment. A healthy ecological environment will provide strong support for agriculture and will be an important foundation and guarantee for agricultural development. However, due to the long-term use of chemical fertilizers or Organic fertilizers and unreasonable fertilization structure have led to the pollution and destruction of the agricultural ecological environment, the decline of soil physical and chemical properties and caused many serious problems. Today, when green agriculture has become the development direction of the world's agriculture, we should vigorously advocate, dev...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 乔长晟王凡李雪罗喆孙芳艳
Owner 天津北洋百川生物技术有限公司
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