Enzymes for starch processing

A technology of amylase and amino acid, applied in the field of peptides, can solve problems such as energy consumption

Active Publication Date: 2008-02-20
NOVOZYMES AS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] From the above discussion it is evident that the traditional starch conversion process is ve

Method used

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  • Enzymes for starch processing

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Embodiment approach

[0094] In a preferred embodiment, the polypeptide comprises a CBM derived from Atathelia rotundum, A. papyrus, Valsaria rubricosa, or A. macroporus. Preferably any polypeptide comprising a CBM amino acid sequence selected from the group consisting of: A. papierae glucoamylase (SEQ ID NO: 92), A. papieriformis glucoamylase (SEQ ID NO: 76), Valsaria rubricosa α - Amylase (SEQ ID NO: 84) and Macroporus alpha-amylase (SEQ ID NO: 88).

[0095] In another preferred embodiment, the polypeptide comprises an α-amylase sequence (SEQ ID NO: 4) derived from Aspergillus oryzae acid α-amylase, preferably wherein the Aspergillus oryzae amino acid sequence comprises one selected from the following group or multiple amino acid substitutions: A128P, K138V, S141N, Q143A, D144S, Y155W, E156D, D157N, N244E, M246L, G446D, D448S, and N450D. Most preferably said polypeptide comprises a catalytic domain having the amino acid sequence shown in SEQ ID NO:6. In a preferred embodiment, the polypeptide f...

Embodiment 1

[0285] Embodiment 1: Construction of the nucleic acid sequence V019 encoding Rhizomucor pusillus (Rhizomucor pusillus) α-amylase and Athelia rolfsii (Athelia rolfsii) glucoamylase CBM

[0286] Vector pLA1 was digested with appropriate restriction endonucleases to excise the region encoding the catalytic domain of the A. niger alpha-amylase. Primers P001 (SEQ ID NO: 104) and P002 (SEQ ID NO: 105) were used to amplify the Rhizomucor pumila α-amylase gene by PCR, and the amplified fragment is shown in SEQ ID NO: 19.

[0287] PCR reaction system:

[0288] DNA fragments were recovered from agarose gels using a Qiagen gel extraction kit. The resulting purified fragments were mixed with the vector digest. The mixed solution was introduced into Saccharomyces cerevisiae to construct expression plasmid pLAV019 by in vivo recombination.

Embodiment 2

[0289] Embodiment 2: the construction of the nucleic acid sequence V022 of encoding giant polyporus (Meripilus giganteus) α-amylase and Athena rotundum glucoamylase CBM

[0290] Primers P003 (SEQ ID NO: 106) and P004 (SEQ ID NO: 107) were used to PCR amplify the polyporus macroporus alpha-amylase gene.

[0291] DNA fragments were recovered from agarose gels using a Qiagen gel extraction kit. The resulting purified fragment was mixed with the vector pLA1 in which the catalytic domain encoding the A. niger α-amylase was excised by digestion with an appropriate restriction endonuclease. The mixed solution was introduced into Saccharomyces cerevisiae to construct expression plasmid pLAV022 by in vivo recombination.

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Abstract

The present invention relates to polypeptides having glucoamylase activity and isolated polynucleotides encoding said polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. The invention also relates to the composition comprising a glucoamylase of the invention as well as the use such compositions for starch conversion processes, brewing, including processes for producing fermentation products or syrups.

Description

[0001] Cross-References to Sequence Listings and Deposited Microorganisms [0002] This application contains information in the form of a Sequence Listing, which is appended to this application, and a data carrier thereof is also filed with this application. Furthermore, the present application relates to deposited microorganisms. The contents of the data carrier and the deposited microorganisms are hereby fully incorporated by reference. [0003] Field of invention [0004] The present invention relates to polypeptides comprising a carbohydrate binding module ("CBM") and an alpha-amylase catalytic domain. In addition, the invention relates to wild-type alpha-amylase polypeptides comprising useful alpha-amylase catalytic domains and / or CBMs, and also to catalytic domain sequences and / or CBM sequences. The invention also relates to the use of these polypeptides in a starch liquefaction process which degrades starch into smaller oligosaccharide and / or polysaccharide fragments. ...

Claims

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Application Information

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IPC IPC(8): C12N9/32C12N1/16C12N15/74C07H21/04C12N5/10
CPCY02E50/17C07K2319/00C12N9/242C12N9/2428C12Y302/01001C12Y302/01003
Inventor 福山志朗松井知子宋子良埃里克·阿兰安德斯·维克索-尼尔森宇田川裕晃刘晔段俊欣吴文平利尼·N·安德森萨拉·兰德维克
Owner NOVOZYMES AS
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