12 results about "Osteoprotegerin" patented technology

The invention provides an application of bifidobacterium animalis subsp. Lactis CP-9 in preparation of a product for promoting bone growth and development or improving bone health. Long-term research and a large number of experiments show that the bifidobacterium animalis subsp. Lactis CP-9 can improve the bone volume fraction, the bone trabecula thickness, the bone density and the bone volume of an organism, and can also effectively improve the growth hormone level and the insulin-like growth factor 1 level, so that bone growth and development are promoted. Meanwhile, the bifidobacterium animalis subsp. Lactis CP-9 can effectively improve the N-terminal propeptide level, osteocalcin level, bone morphogenetic protein level and osteoprotectin level of I-type procollagen in a body, and remarkably reduce the amino-terminal peptide level of the I-type collagen and the ligand level of nuclear factor kappa B receptor activating factor, so that osteoblast differentiation is promoted, osteoclast differentiation is inhibited, and osteoclast differentiation is inhibited. Furthermore, bone formation is promoted, bone resorption is inhibited, bone metabolism is improved, and finally the effect of improving bone health is achieved.

The invention provides an osteoprotegerin recombinant protein, a monoclonal antibody and application of the osteoprotegerin recombinant protein and the monoclonal antibody, and relates to the technical field of biological detection, and the amino acid sequence of the recombinant protein is as shown in SEQ ID No.1. The invention provides the osteoprotegerin recombinant protein, a monoclonal antibody pair with high specificity is screened on the basis of the osteoprotegerin recombinant protein, an osteoprotegerin double-antibody sandwich method is established, the osteoprotegerin protein can be specifically detected, the sensitivity is high, a simple and convenient biological detection method is provided, and the application prospect is relatively good.

The present invention provides an application of animal Bifidobacterium lactis subspecies CP-9 in the preparation of a product that promotes bone growth and development or improves bone health. After long-term research and a large number of experiments, the present invention found that animal Bifidobacterium lactis subspecies CP-9 can improve the body's bone volume fraction, trabecular thickness, bone density and bone mass, and can also effectively increase the level of growth hormone and insulin-like growth factor 1, thereby promoting bone growth and development. At the same time, animal Bifidobacterium lactis subspecies CP-9 can effectively increase the level of type I procollagen N-terminal propeptide, osteocalcin level, bone morphogenetic protein level, and osteoprotegerin level in the body, and significantly reduce the level of type I collagen amino-terminal peptide and nuclear factor κB receptor activator ligand level, thereby promoting osteoblast differentiation, inhibiting osteoclast differentiation, and then promoting bone formation, inhibiting bone resorption, improving bone metabolism, and ultimately achieving the effect of improving bone health.

The application discloses a 3D paper-based microfluidic osteoprotegerin detection device, a preparation method and application, and relates to the paper-based microfluidic field.The device comprises a paper-based flow guide layer, a paper-based detection layer and an absorption layer;the paper-based flow guide layer is provided with a hydrophilic pattern area with a periphery hydrophobic based on a wax printing heating method;the hydrophilic pattern area is used for receiving a mixed fluid;the mixed fluid comprises a sample to be detected and an antibody-color developing solution;the paper-based detection layer is provided with a standard indication area based on a wax printing heating method, and a detection area corresponding to the hydrophilic pattern area and used for receiving the mixed fluid permeated through the hydrophilic pattern area;the detection area is fixed with a human osteoprotegerin antibody, and the standard indication area is dyed with a standard concentration detection color development used for indicating a human osteoprotegerin concentration gradient;the absorption layer is used for collecting the waste liquid permeated through the paper-based detection layer after detection.Compared with the prior art, the application can realize instant detection of a human osteoprotegerin sample, has low production cost, high portability, no dependence on large instruments, and wide application scenarios.

The disclosure provides, in various aspects, methods of treating cancer in a subject in need of treatment therefor, comprising administering to the subject a therapeutically effective amount of an osteoprotegerin inhibitor, wherein the osteoprotegerin inhibitor inhibits osteoprotegerin binding to TRAIL. In further aspects, the disclosure provides methods of achieving a therapeutic outcome in a subject in need of treatment, wherein the method comprises the administration to the subject of a treatment which inhibits OPG-mediated suppression of tumor immunity.

The invention relates to the technical field of biological treatment of bone metabolic diseases, and discloses an application of a bone-strengthening and blood-generating oral liquid in preparation of a medicine for treating osteoporotic fracture.The application includes the steps that when the medicine is prepared, active components in the bone-strengthening and blood-generating oral liquid are combined with a Wnt signal transduction complex in osteoblasts in a targeted mode, and the bone-strengthening and blood-generating oral liquid is obtained; the phosphorylation activity of glycogen synthase kinase-3 is inhibited, so that-catenin enters a nucleus, Runx2 expression is started, and the synthesis rate of the N-terminal propeptide of the type I procollagen is increased; according to the method, a positive anabolism window is established in an original callus formation period of fracture healing by constructing an osteogenesis and osteoclast activity two-way decoupling biological regulation mechanism, so that a positive anabolism effect is achieved, and a positive anabolism effect is achieved. Bone metabolism pathological deviation in an osteoporosis state is corrected, secondary absorption of callus is inhibited while high osteogenic activity is maintained, and biomechanical fracture load of bones after fracture healing is improved.

This invention discloses a strain of *Lactobacillus gasseri* that reduces the risk of osteoporosis caused by low estrogen levels and its applications, belonging to the field of microbial technology. The *Lactobacillus gasseri* CCFM1255 provided by this invention can increase serum estradiol levels in ovariectomized rats, increase serum calcium levels in ovariectomized rats, and decrease serum procollagen type I N-terminal propeptide (PINP) and osteoprotegerin (OPG) levels in ovariectomized rats, thus alleviating bone turnover rate. The elevated levels of IL-10, TNF-α, and IL-6 in the serum of ovariectomized rats were also effectively inhibited. Furthermore, CCFM1255 can increase the expression of CYP19 and estrogen receptor ESR1 in the adipose tissue of ovariectomized rats. The *Lactobacillus gasseri* CCFM1255 of this invention is a food-safe strain and can be used to prepare food, functional food, or pharmaceuticals, with broad application prospects.

The application discloses a Streptococcus salivarius CCFM1399 and its postbiotic for relieving bone resorption and periodontal tissue damage, and belongs to the technical field of microorganisms. The Streptococcus salivarius CCFM1399 provided by the application is separated from oral plaque of healthy teenagers, has the effects of relieving bone resorption and resisting inflammation, and specifically has the following effects: improving the expression of osteoprotegerin (OPG) protein level and gene level of MC3T3-E1 cells in vitro; reducing the alveolar bone resorption amount of orthodontic bone resorption rats, reducing the number of osteoclasts in alveolar bone, and improving the OPG level in serum of rats; reducing the secretion of inflammatory factors IL-6, TNF-alpha and MPO of orthodontic bone resorption rats; and improving the pathological condition of alveolar bone tissue of orthodontic bone resorption rats. Therefore, the Streptococcus salivarius has great application prospect in products for relieving bone resorption and assisting in inhibiting periodontal tissue inflammation.

Hollow hydroxyapatite microparticles are provided for sustained delivery of an agent that provides osteoprotegerin anti-osteolytic activity for treatment of post-orthodontic relapse and bone disorders. In particular, methods of using such microparticles for post-orthodontic tooth stabilization and bone regeneration and repair are provided.

According to the composite probiotic preparation and the application thereof in preparation of products for improving body metabolism, 15 specific probiotic strains are compounded, all the strains cooperate to adjust body nutrition metabolism and bone tissue metabolism at the same time, and the problem that an existing probiotic preparation is single in function is solved. In the aspect of nutrition metabolism regulation, the levels of serum total protein, albumin and globulin can be increased, the content of essential amino acids such as threonine, methionine and tryptophan in serum can be increased, the protein synthesis capability of the body can be enhanced, the liver synthesis function can be improved, and the nutrition metabolism efficiency of the body can be improved; in the aspect of bone tissue metabolism regulation, the composition can improve the levels of osteocalcin and osteoprotegerin, promote osteoblast activity, inhibit bone resorption, improve the expression levels of TRPV6 and NCX1 in intestinal epithelial cells, enhance intestinal calcium absorption and transepithelial transport efficiency, reduce the thickness variance of tibia trabecula, improve the tibia microstructure and maintain the uniformity and steady state of a bone tissue structure.

The present invention provides a medicinal and edible composition for preventing and treating osteoporosis and a preparation method thereof. The raw materials of the medicinal and edible composition are: 15-24 parts of Rehmannia root, 12-15 parts of Cornus officinalis, 12-20 parts of Chinese yam, 15-20 parts of Astragalus root, 3-10 parts of Chinese angelica root, and 10-20 parts of Eucommia ulmoides leaves; the medicinal and edible composition of the present invention increases osteoprotegerin, reduces type I collagen C-terminal peptide and tartrate-resistant acid phosphatase, thereby promoting osteoblast differentiation, inhibiting osteoclast resorption, regulating bone metabolism balance, increasing femoral maximum load, and improving bone density and bone strength. The medicinal and edible composition of the present invention is highly safe and can be developed into medicines, health products, or functional foods for the prevention and treatment of osteoporosis.

The invention provides an application of the lactobacillus rhamnosus GOLDGUT-M520 in preparation of products for promoting nutrition digestion and absorption and improving bone health, and the lactobacillus rhamnosus GOLDGUT-M520 has dual functions: on one hand, the lactobacillus rhamnosus GOLDGUT-M520 can promote digestion of starch, protein and fat and absorption of mineral substances such as zinc, iron and calcium; the intestinal form is improved, the body protein and amino acid level is improved, and the intestinal flora balance is regulated; on the other hand, the absorption efficiency of intestinal calcium can be improved, the levels of osteocalcin and osteoprotegerin are improved, and the microstructure and mechanical properties of bone tissues are improved. The strain can be applied to the fields of food, health care products and medicines, products with dual functions are developed, dual guarantee is provided for body nutrition supply and bone homeostasis, health collaborative maintenance is achieved, and the application value is remarkable.