SARS virus antibody detecting method, rapid diagnosis kit and preparation method

A technology for rapid diagnosis and antibody detection, applied in the field of diagnosis and detection of SARS coronavirus antibodies, can solve the problems of difficulty in virus antigen preparation, inability to mass-produce, poor specificity, etc., and achieve the effects of simple preparation method, simple operation and good stability.

Inactive Publication Date: 2005-01-26
LANZHOU YAHUA BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above immunoassay techniques all use SARS virus-infected cells as antigens, and the cultured virus ...

Method used

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  • SARS virus antibody detecting method, rapid diagnosis kit and preparation method
  • SARS virus antibody detecting method, rapid diagnosis kit and preparation method
  • SARS virus antibody detecting method, rapid diagnosis kit and preparation method

Examples

Experimental program
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Embodiment 1

[0030] A kind of SARS coronavirus mutant strain antibody detection method comprises the steps: (1) the anti-human Ig antibody of colloidal gold labeling comprises IgG and IgM to be carried on glass fiber or non-woven cloth carrier: and with colloidal gold labeling antibody The detection carrier that is connected to the carrier is coated with the detection line formed by the genetically engineered SARS coronavirus variant antigen and the control line formed by the anti-mouse IgG antibody: (2) get human serum and drop it on the carrier of the colloidal gold-labeled antibody, if the serum contains The SARS coronavirus mutant antibody forms a detection line and a control line on the detection carrier, which is positive, otherwise it is negative.

[0031] refer to figure 1 A gold-labeled rapid diagnostic kit for antibodies to variants of SARS coronavirus is equipped with a gold-labeled detection plate 1 for antibodies to variants of SARS coronavirus.

[0032] refer to figure 2 ,...

Embodiment 2

[0038] The SARS coronavirus variant antibody detection method and its gold-labeled rapid diagnostic kit are included with the example.

[0039] The preparation method of the kit is the same as the example except that the colloidal gold-labeled antibody is an anti-human IgM monoclonal antibody.

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Abstract

This invention relates to a detection mode of SARS virus antibody and a preparing method of the box for rapid diagnosis reagent. The checking method includes that (1) putting antihuman Ig antibody marked by colloidal gold in the carrier, and coating detecting thread made of variant antigen of SARS coronavirus and controlling thread made of antipest IgG antibody in the detecting carrier connecting with the marked one. (2) putting human serum in the marked carrier. The box for rapid diagnosis reagent includes checkerboard marked by variant antibody of SARS coronavirus, which comprises water-absorbing layer of adding-kind edge, detecting layer and water-absorbing layer of water-uptake edge. There is antihuman Ig antibody layer between detecting layer and water-absorbing layer of water-uptake edge, and in the detecting layer there is detecting thread made of variant antigen of SARS coronavirus and controlling thread made of antipest IgG antibody coating.

Description

technical field [0001] The invention relates to a method for diagnosing and detecting antibodies against SARS coronavirus, a gold-labeled rapid diagnostic kit for antibodies against SARS coronavirus used in the method, and a preparation method thereof. Background technique [0002] Severe Acute Respiratory Syndrome (SARS) is a sudden human infectious disease that recently occurred in many countries in the world, and it is a sudden disaster faced by mankind. The prevention and treatment of SARS has become the most important task at present. The vast number of medical workers are fighting against SARS. Scientists from all over the world are studying the prevention and treatment methods of SARS, including diagnostic methods, therapeutic drugs and preventive vaccines. The US "Science" magazine published two series of research papers on the SARS virus genome on May 1. This is the first batch of peer-reviewed SARS virus genome research results. Two scientific research teams compo...

Claims

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Application Information

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IPC IPC(8): C12Q1/04G01N33/53G01N33/531G01N33/543G01N33/569
CPCY02A50/30
Inventor 李克生杜惠芬刘奇
Owner LANZHOU YAHUA BIOTECH
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