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Rabies virus antibody (IgG) enzyme-linked immunoassay kit and detection method thereof

An enzyme-linked immunosorbent assay, rabies virus technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of inconvenient use and narrow application area, and achieve the effect of low interference, high sensitivity and wide application area.

Active Publication Date: 2015-07-22
成大生物(本溪)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of these kits is relatively narrow. For the detection of IgG antibodies of each type of animal, it is necessary to prepare corresponding secondary antibodies and anti-enzyme-labeled conjugates, which is inconvenient to use; at the same time, the sensitivity of the kits In terms of performance, there is still room for improvement, and more accurate detection can be carried out, so that the probability of false positive and false negative results is greatly reduced

Method used

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  • Rabies virus antibody (IgG) enzyme-linked immunoassay kit and detection method thereof
  • Rabies virus antibody (IgG) enzyme-linked immunoassay kit and detection method thereof
  • Rabies virus antibody (IgG) enzyme-linked immunoassay kit and detection method thereof

Examples

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Embodiment 1

[0061] Example 1: Preparation of an enzyme-linked immunoassay kit for detection of rabies virus antibody (IgG) and detection of human serum.

[0062] Rabies virus antibody (IgG) enzyme-linked immunoassay kit, mainly composed of purified rabies virus antigen coated plate, concentrated diluent, enzyme conjugate working solution, substrate chromogenic solution A, substrate chromogenic solution B, concentrated washing solution, negative and positive controls, and stop solution. Including the following:

[0063] 1) 96-well ELISA plate, coated with 0.05mol / L pH9.6 carbonate bicarbonate buffer, dilute rabies virus purification solution, and the final concentration of protein content is 0.3μg / ml;

[0064] 2) 10 times concentrated diluent: KH 2 PO 4 : 0.4g; Na 2 HPO 4 12H 2 O: 5.8g; NaCl: 16.0g; KCl: 0.4g; dissolve in 100ml of distilled water and adjust the pH to 7.2. Add Tween 20 0.5ml, filter with 0.22μm filter membrane;

[0065] 3) Enzyme conjugate working solution: horserad...

Embodiment 2

[0107] Example 2: Preparation of rabies virus antibody (IgG) ELISA kit and detection of dog serum.

[0108] Rabies virus antibody (IgG) enzyme-linked immunoassay kit, mainly composed of purified rabies virus antigen coated plate, concentrated diluent, enzyme conjugate working solution, substrate chromogenic solution A, substrate chromogenic solution B, concentrated washing solution, negative and positive controls, and stop solution. Including the following:

[0109] 1) 96-well ELISA plate, coated with 0.05mol / L pH9.6 carbonate bicarbonate buffer solution, diluted rabies virus purification solution, the final concentration of protein content is 0.1μg / ml;

[0110] 2) 10 times concentrated diluent: KH 2 PO 4 : 0.4g; Na 2 HPO 4 12H 2 O: 5.8g; NaCl: 16.0g; KCl: 0.4g; dissolve in 100ml of distilled water and adjust the pH to 7.2. Add Tween 20 0.5ml, filter with 0.22μm filter membrane;

[0111] 3) Enzyme conjugate working solution: horseradish peroxidase-labeled protein A con...

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Abstract

The invention provides a rabies virus antibody (IgG) enzyme-linked immunoassay kit and a detection method thereof. The detection kit is composed of a purified rabies virus antigen coated microporous plate, enzyme labeled SPA and other reagents. The detection method adopts an indirect method principle to detect the rabies virus IgG antibody in human or animal serum or blood plasma, and is suitable for rabies vaccine immunized serology effect evaluation and epidemiology investigation. An enzyme labeled antibody applied in the invention is a Staphylococal protein A (SPA), and the SPA can be combined with an Fc fragment in IgG molecules in human or mammal serum, so the kit provided by the invention has all the characteristics of an ELISA kit, can be used for human rabies virus antibody detection, and can also be used for detecting the immune effect of various species of animals.

Description

technical field [0001] The invention relates to a method for detecting rabies virus antibody (IgG), in particular to a rabies virus antibody (IgG) ELISA detection kit and a detection method thereof, which belong to the technical field of ELISA. The kit of the invention uses an indirect method to measure the rabies virus antibody (IgG) content in a sample, and can be used for monitoring the immune effect of rabies vaccine and determining the immune status of individuals. Background technique [0002] Rabies, commonly known as mad dog disease, is a zoonotic infectious disease, the pathogen is rabies virus; all warm-blooded animals, including humans, may be infected. It can cause acute encephalitis and peripheral nerve inflammation in animals, and the mortality rate after the onset is as high as 100%. Infected persons who have not received vaccine immunity are almost certain to die when neurological symptoms appear. The usual cause of death is that the central nervous system (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/56983G01N33/6893G01N2333/145
Inventor 苏文全曾祥伟赵森汪婷于铁富袁德明卫广森
Owner 成大生物(本溪)有限公司
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