HIV viral antibody/antigen diagnostic reagent kit and preparing method thereof and detecting method
A diagnostic kit and antigen technology, applied to measuring devices, material analysis through observation of the influence of chemical indicators, instruments, etc., can solve the problems of simultaneous detection, long window period, and prone to missed detection, etc., to achieve shortening Window period, improving sensitivity and accuracy, and the effect of improving sensitivity
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[0037] Component examples of the human immunodeficiency virus type 1+2 antibody / P24 antigen diagnostic kit of the present invention:
[0038] (1), pre-coated enzyme-linked plate coated with HIV antigen and P24 monoclonal antibody;
[0039] (2), with biotin (Biotin) labeled rabbit anti-P24 polyclonal antibody;
[0040] (3), horseradish peroxidase (HRP) labeled avidin (Streptavidin) and HIV antigen conjugate;
[0041] (4), the washing liquid containing the phosphate buffered saline of Tween;
[0042] (5), color developing solution A of citrate buffer containing hydrogen peroxide;
[0043] (6), citrate buffer solution B containing tetramethylbenzidine;
[0044] (7), stop solution containing sulfuric acid solution;
[0045] (8), sample diluent containing phosphate buffer;
[0046] (9), negative control normal human serum;
[0047] (10), HIV antibody positive control serum;
[0048] (11), P24 antigen positive control serum.
[0049] Embodiments of the preparation method of ...
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