HIV viral antibody/antigen diagnostic reagent kit and preparing method thereof and detecting method

A diagnostic kit and antigen technology, applied to measuring devices, material analysis through observation of the influence of chemical indicators, instruments, etc., can solve the problems of simultaneous detection, long window period, and prone to missed detection, etc., to achieve shortening Window period, improving sensitivity and accuracy, and the effect of improving sensitivity

Inactive Publication Date: 2005-09-28
北京科卫临床诊断试剂有限公司
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Problems solved by technology

[0003] The purpose of the present invention is to provide a HIV virus antibody / antigen diagnostic kit and its preparation method and detection method, which solves the problem that the prior art can only detect one of the antibody or the antigen, and the two cannot be detected simultaneously, so that the detection window period Long, prone to technical problems of missed detection

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  • HIV viral antibody/antigen diagnostic reagent kit and preparing method thereof and detecting method

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Embodiment Construction

[0037] Component examples of the human immunodeficiency virus type 1+2 antibody / P24 antigen diagnostic kit of the present invention:

[0038] (1), pre-coated enzyme-linked plate coated with HIV antigen and P24 monoclonal antibody;

[0039] (2), with biotin (Biotin) labeled rabbit anti-P24 polyclonal antibody;

[0040] (3), horseradish peroxidase (HRP) labeled avidin (Streptavidin) and HIV antigen conjugate;

[0041] (4), the washing liquid containing the phosphate buffered saline of Tween;

[0042] (5), color developing solution A of citrate buffer containing hydrogen peroxide;

[0043] (6), citrate buffer solution B containing tetramethylbenzidine;

[0044] (7), stop solution containing sulfuric acid solution;

[0045] (8), sample diluent containing phosphate buffer;

[0046] (9), negative control normal human serum;

[0047] (10), HIV antibody positive control serum;

[0048] (11), P24 antigen positive control serum.

[0049] Embodiments of the preparation method of ...

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Abstract

The present invention is HIV virus antibody / antigen diagnosis kit and its preparation process and detection method. The kit includes pre-coated enzyme-linked board with coated HIV antibody and P24 monoclonal antibody, rabbit anti-P24 polyclonal antibody labeled with biotin, conjugate of horseradish peroxidase labeled avidin and HIV antigen, detergent solution of phosphate buffer containing Tween, developing solution A of citrate buffer containing hydrogen peroxide, developing solution B of citrate buffer containing tetramethyl benzidine, terminating solution containing sulfuric acid solution, sample diluting solution containing phosphate buffer, normal human serum as positive contrast, HIV antibody serum as positive contrast, and P24 antigen serum as positive contrast. The present invention can detect HIV antibody and P24 antigen simultaneously in raised specificity and sensitivity and is suitable for diagnosis of human immune deficiency virus antibody.

Description

(1) Technical field [0001] The invention relates to a kit for detecting whether virus antibodies and antigens exist in a sample, in particular to a diagnostic kit for simultaneously detecting human immunodeficiency virus type 1+2 antibodies and P24 antigens and a preparation method and a detection method thereof. (2) Background technology [0002] Human immunodeficiency virus (HIV) is the pathogen of AIDS (AIDS), divided into HIV1 and HIV2. AIDS is one of the most important infectious diseases currently endangering human health. It is mainly transmitted through sexual contact, blood, intravenous drug abuse and mother-to-child transmission. According to estimates by the World Health Organization, more than 80 million people have been infected with HIV, and more than 20 million people have been killed by AIDS. At present, there is no effective way to cure AIDS, but through the detection of human immunodeficiency virus 1 in blood samples The physiological parameters of +2 anti...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/535G01N33/543G01N33/569
Inventor 王保君冯长访马晓晖
Owner 北京科卫临床诊断试剂有限公司
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