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SARS-CoV-2 antigen polypeptides and application thereof

An antigen peptide, sars-cov-2 technology, applied in the field of immunobiology, can solve the problem of undiscovered specific peptide detection reports, etc., and achieve the effects of reducing detection cost, improving specificity, and improving sensitivity

Active Publication Date: 2020-11-24
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no optimized specific peptides have been found for the detection of SARS-CoV-2 patients

Method used

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  • SARS-CoV-2 antigen polypeptides and application thereof
  • SARS-CoV-2 antigen polypeptides and application thereof
  • SARS-CoV-2 antigen polypeptides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 The specific antigenic polypeptide of the present invention has extremely high antigenicity

[0069] In order to verify that the designed four specific antigen polypeptides have extremely high antigenicity, that is, the ability to bind to corresponding antibodies, the designed four specific antigen polypeptides were subjected to ELISA reactions with serum samples of different dilutions.

[0070] Serum samples from COVID-19 patient #1 were taken and diluted 1:500, 1:1000, 1:2000, 1:4000 and 1:8000, respectively. Serum from normal people was used as a control, and it was also diluted by corresponding multiples. Then carry out antigen-antibody reaction with S1, S2, N1, N2 four antigen polypeptides respectively, and use enzyme-linked immunoassay (ELISA) to detect the reaction intensity of the antibody to the four polypeptides.

[0071] Experimental results such as Figure 1-4 shown. It can be seen that there are significant differences between the OD450 values ...

Embodiment 2

[0072] Example 2 The application of the specific antigen polypeptide of the present invention in detecting the antibody level of patients with new crown has good universality

[0073] In order to verify that the four antigen polypeptides designed in the present invention can generate universal antigen-antibody responses to different patient sera, this example uses serum samples from three patients for experiments.

[0074] Serum samples from No. 1, No. 2, and No. 3 new crown patients (#1, #2, #3) were taken for experiments, and normal human serum was used as a control. Except that the serum sample of No. 1 new crown patient (#1) was diluted at 1:4000 when reacting with the N2 antigen polypeptide, the serum samples of other patients and normal people were diluted at 1:2000.

[0075] Experimental results such as Figure 5-8 shown. It can be seen that the serum samples from three COVID-19 patients were detected by ELISA with the four antigens, and there were significant differe...

Embodiment 3

[0076] Example 3 The combination of specific antigen polypeptides of the present invention can be used for antibody detection

[0077] In order to verify that the combination of the four antigen polypeptides designed in the present invention can also be used for antibody detection, in this example, the four antigen polypeptides were combined and then tested with serum samples from three patients.

[0078] Combination 1: Combination of S1 antigen polypeptide and S2 antigen polypeptide, such as Figure 9 shown.

[0079] Combination 2: N1 antigen polypeptide and N2 antigen polypeptide combination, such as Figure 10 shown

[0080] Combination 3: S1, S2, N1, N2 antigen polypeptides are combined together, such as Figure 11 shown.

[0081] In order to ensure the comparability with polypeptides used alone, the total mass of each antigen polypeptide combined in the experiment is the same as that of one polypeptide alone, that is, the total mass of antigen polypeptides per well is...

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PUM

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Abstract

The invention discloses SARS-CoV-2 antigen polypeptides and application thereof. The invention relates to four SARS-CoV-2 polypeptide antigens and application thereof. The amino acid sequences of thefour SARS-CoV-2 polypeptide antigens are SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or derivative sequences thereof. The epitopes screened out in the invention can be used for detecting anSARS-CoV-2 virus antibody, are high in specificity and accuracy, have an application value in clinical diagnosis, and play a positive and effective role in preventing and controlling SARS-CoV-2 virusinfection.

Description

technical field [0001] The invention belongs to the technical field of immunobiology, and in particular relates to a SARS-CoV-2 antigen polypeptide and an application thereof. Background technique [0002] Novel coronavirus pneumonia (COVID-19) is caused by the 2019 novel coronavirus (SARS-CoV-2). On January 30, 2020, the World Health Organization declared COVID-19 as the sixth public health emergency of international concern. SARS-CoV-2 is an enveloped, single-stranded positive-sense RNA virus belonging to the genus Beta of the coronavirus family. The genome size of SARS-CoV-2 ranges from 29.8 kb to 29.9 kb, and its genome structure follows specific genetic features of known coronaviruses. More than two-thirds of the genome in the 5' is orf1ab, encoding the orf1ab polyprotein, while the 3' one-third consists of genes encoding structural proteins, including spike protein (S), envelope protein (envelope protein , E), membrane glycoprotein (membraneglycoprotein, M) and nucl...

Claims

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Application Information

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IPC IPC(8): C07K14/165G01N33/569G01N33/543G01N33/535
CPCC07K14/005G01N33/56983G01N33/543G01N33/535C12N2770/20022G01N2333/165
Inventor 徐可郑雨澄唐笛霄宋坤
Owner WUHAN UNIV
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