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Senecavirus ELISA antibody detection kit and preparation method and application

An antibody detection and kit technology, which is applied in the field of Seneca virus ELISA antibody detection kit and its preparation field, achieves the effects of stable results, good specificity and repeatability, and high sensitivity

Inactive Publication Date: 2018-11-06
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no corresponding ELISA method established in China

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  • Senecavirus ELISA antibody detection kit and preparation method and application

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Embodiment Construction

[0050] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts fall within the protection scope of the present invention.

[0051] Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terminology used in the description of the present invention in this specification is only for the purpose of describing specific embodiments, and is not used to limit the present invention. The term "and / or" used in this specification includes any and all combinations o...

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Abstract

The invention discloses a Senecavirus ELISA antibody detection kit and a preparation method and application. The Senecavirus ELISA antibody detection kit comprises a Senecavirus VP1 protein-precoatedELISA plate, a blocking solution, a diluted sample solution, an enzyme conjugate, a concentrated washing solution, an enzyme substrate solution and a stopping solution. Senecavirus VP1 protein is usedas a coating antigen for the first time, a kit capable of detecting Senecavirus is established, and the antibody level of the Senecavirus in serum can be rapidly detected; the detection kit is high in sensitivity, good in specificity, good in repeatability and stable in result; the detection kit can be applied to monitoring of the antibody level of the Senecavirus so as to understand the status of the Senecavirus antibody in a whole pig herd; in addition, the detection kit uses an Escherichia coli expression system, and has the advantages of economy and cheapness.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a Seneca virus ELISA antibody detection kit and a preparation method and application thereof. Background technique [0002] Seneca virus disease is an animal infectious disease caused by Senecavirus A (SVA) of the Picornaviridae Senecavirus genus. It mainly infects pigs. Pigs of different ages are susceptible, but different The pathogenicity of the strains is significantly different, and the clinical symptoms caused by them are also different. Early isolates often lead to subclinical infection. In recent years, isolates usually cause foot-and-mouth disease-like clinical symptoms. Blisters and ulcers appear on the snout and hooves of sick pigs, and the mortality rate of newborn piglets increases significantly. At present, the disease is mainly prevalent in Canada, Brazil, the United States and other countries; in the summer of 2015, an outbreak of SVA was confirmed f...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/558G01N33/56983G01N2333/09
Inventor 郭慧琛孙世琪白满元韩世充董虎宋品魏衍全张韵殷宏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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