Process for expelling chrysanthemum flower viroid by cryogenic processing

A low-temperature treatment and virus-like technology, which is applied in the field of plant purification, rejuvenation and breeding, can solve problems such as death, plant dryness, and inability to remove chrysanthemum-like virus, and achieves the effect of simple process method and good effect.

Inactive Publication Date: 2007-03-14
河北省农林科学院经济作物研究所
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Problems solved by technology

Heat treatment plus stem tip culture is currently the most commonly used method for removing plant viruses, and the effect is good, but this method has great li...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method for removing chrysanthemum-like viruses by low temperature treatment includes the following steps:

[0023] (1) Low temperature treatment: The chrysanthemum root stubble that is overwintering under natural conditions is covered with 3-5 cm soil and covered with a small plastic shed. When the root temperature is in the range of -2℃-3℃, take the bud point of the chrysanthemum just budding Or root tiller bud. ;

[0024] (2) Disinfection treatment: Wash the bud points, root tillers or apical buds obtained in step (1) with soapy water, and then use 70% alcohol on the ultra-clean workbench to sterilize the surface for 10-20 seconds, 0.1% Sterilize the mercury solution for 1 to 3 minutes, then rinse with sterile water 3 to 5 times, and absorb the water on the surface of the buds with sterile filter paper. ;

[0025] (3) Cut the shoot tip culture: cut the shoot point, root tiller bud or apical bud shoot tip obtained in step (2) and inoculate it in 1 / 3MS+6-BA1.0mg / kg+IBA0...

Embodiment 2

[0029] The step (1) low temperature treatment in this example is to select the robust growth of chrysanthemum test-tube bottle seedlings and place them in a low-temperature incubator with light transmission, and perform low-temperature cold treatment for 90-120 days at a temperature of 4°C-6°C. Then take the top bud. The remaining steps are the same as in Example 1.

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Abstract

The invention relates to a method for using low-temperature treatment to remove chrysanthemum virus. Wherein, it comprises low-temperature treatment, disinfection, cutting off and cultivating stem sharp, viroid detecting, cultivating neuter chrysanthemum. The invention can effectively remove chrysanthemum virus, with simple process and better effect.

Description

Technical field [0001] The invention belongs to the purification and rejuvenation and breeding of plants, and specifically is a method for removing chrysanthemum viruses by using low temperature treatment. Background technique [0002] The chrysanthemum virus is a mycoplasma virus. The virus has a high temperature, the infected chrysanthemum plant is dwarfed, and the leaves are partially chlorotic or withered. When the virus infection is severe, the leaves are dry and the whole plant is dead. The incidence rate is 20-30%. Heat treatment plus shoot tip culture is currently the most commonly used method to remove plant viruses, and the effect is good. However, this method has great limitations in detoxification of chrysanthemums. One is that it cannot remove chrysanthemum viruses, and the other is that heat treatment is easy to cause The plants are dry and dead. Summary of the invention [0003] The purpose of the present invention is to provide a method for removing chrysanthemum...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G1/00A01G7/00
Inventor 谢晓亮田伟周巧梅吴志明刘铭温春秀
Owner 河北省农林科学院经济作物研究所
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