Application of specific protein WIPI1 in early myocardial infarction diagnostic reagent and kit

A diagnostic reagent and a specific technology used in the field of biomedicine to achieve stable and reliable results, direct detection results, and avoid missed detections

Inactive Publication Date: 2020-11-03
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The WIPI1 ge...

Method used

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  • Application of specific protein WIPI1 in early myocardial infarction diagnostic reagent and kit
  • Application of specific protein WIPI1 in early myocardial infarction diagnostic reagent and kit
  • Application of specific protein WIPI1 in early myocardial infarction diagnostic reagent and kit

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Embodiment

[0029] The experimental mode in this example is to detect protein changes within the very early stage (1 hour) of myocardial infarction through DIA-MS proteome sequencing. For the experimental mode diagram, see figure 1 .

[0030] Modeling of animal experiments: In the present invention, in order to search for proteins and genes that undergo significant changes in the process of myocardial infarction, the model mouse c57 was used to construct a myocardial infarction model. Firstly, the myocardial infarction model was constructed by ligation of the left anterior descending coronary artery. See the effect after ligation figure 2 1 hour after myocardial infarction, the infarction effect was detected, and a large area of ​​the apex of the heart was seen to be white, implying that this part of the tissue was ischemic in a large area, which proved that the infarction experiment was successful.

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Abstract

The invention relates to application of a specific protein WIPI1 in an early myocardial infarction diagnostic reagent, the amino acid sequence of the specific protein WIPI1 is shown as SEQ ID NO.1, and during specific detection, after myocardial damage, the content of the specific protein WIPI1 in peripheral blood is increased. Compared with the prior art, the diagnostic reagent provided by the technical scheme of the invention is beneficial to early diagnosis, can reflect the severity of myocardial damage, and can be used as an index for early myocardial infarction diagnosis and prediction, and the detection method is relatively simple and easy to operate, has relatively good detection sensitivity, and has relatively small system errors; the application of the specific protein WIPI1 codedby the WIPI1 gene in a myocardial damage diagnosis reagent can be widely popularized; the kit disclosed by the invention is simple in detection method, low in cost, direct and reliable in detection result and suitable for large-scale screening and diagnosis.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application and kit of a specific protein WIPI1 in early myocardial infarction diagnostic reagents. Background technique [0002] Currently, the main biochemical markers for detection of acute myocardial infarction (AMI) are creatine phosphokinase isoform (CK-MB), lactate dehydrogenase (LDH), cardiac troponin (cTnT) and cardiac troponin I (cTnI). Wait. CK-MB and LDH are widely distributed in the body, so their specificity is low, and many diseases can cause their elevation, and their maintenance time in the blood is short; High, prone to cross-reaction. cTnI has the advantages of high specificity, early appearance, high sensitivity, and long duration in the blood, and can be used as an early diagnostic indicator for AMI. Therefore, the current kit detection methods mainly focus on cardiac troponin I (cTnI). [0003] The detection methods of cardiac troponin I (cTnI) include enzy...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/47G01N2800/324G01N2800/52
Inventor 李觉
Owner TONGJI UNIV
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