Myocardial infarction diagnostic marker composition

A technique for myocardial infarction, mir-892b, applied in the field of disease diagnosis

Inactive Publication Date: 2017-11-03
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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Problems solved by technology

However, the expression and possible role of miRNAs in acute myocardial infarction are less studied, previous studies have shown that miR-30a is associated with cardiac hypertrophy, miR-195 target genes regulate apoptosis, cell proliferation and cell cycle, they may Potential clinical value as a biomarker for the diagnosis of acute myocardial infarction, but these are far from enough, more candidate molecular markers are needed for accurate clinical diagnosis and treatment

Method used

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  • Myocardial infarction diagnostic marker composition
  • Myocardial infarction diagnostic marker composition
  • Myocardial infarction diagnostic marker composition

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Experimental program
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Effect test

Embodiment 1

[0045] The collection of embodiment 1 sample and total RNA extraction

[0046] The peripheral blood of patients with myocardial infarction and 6 cases of healthy controls were collected in the hospital from March 2015 to September 2016. Diagnostic criteria for acute myocardial infarction: developed according to the diagnostic criteria recommended by the third "Global Unified Definition of Myocardial Infarction" in 2012. An increase and / or decrease in the level of myocardial necrosis markers (mainly troponin) was detected, exceeding the 99th percentile value of the upper limit of the reference value at least once, and accompanied by at least one of the following ischemic symptoms.

[0047] 1) Symptoms of myocardial ischemia;

[0048] 2) New ischemic ECG changes;

[0049] 3) Pathological Q waves appear in the electrocardiogram;

[0050] 4) Imaging evidence shows new loss of myocardial activity or new local wall motion abnormality;

[0051] 5) Coronary angiography or autopsy ...

Embodiment 2

[0053] Example 2 Sequencing, data analysis and electronic verification

[0054] Sequencing: The miRNA was sequenced using llumina Hiseq2500 / Miseq second-generation high-throughput sequencing technology, and the data processing was completed through the processes of removing adapters, removing low-quality, and decontaminating to obtain the final data. The miRNA sequencing raw data was background-corrected by transcriptome data analysis software, and then the t-test was performed to obtain the P value, and then the Fisher test was used to combine the P values ​​to screen for differentially expressed miRNAs.

[0055] Finally, miR-1468-5p, miR-892b, and miR-1274b were selected from the candidate differentially expressed miRNAs for later experimental verification.

Embodiment 3

[0056] Embodiment 3 Electronic Verification

[0057] Electronic verification: 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145-GPL6884) and 2 sets of miRNA data sets (GSE61741, GSE31568) were screened from the GEO (Gene Expression Omnibus) database, and 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145 -GPL6884) has a total of 13,680 genes. We calculated and merged the effect value by using the metaMA package and using the combined P-value method to obtain 612 (FDR1) were found. The results showed that the electronic verification results were consistent with the expression trend of the sequencing results.

[0058] The method for evaluating the efficacy of a single miRNA molecule or a diagnostic model is to establish a receiver operating characteristic (ROC) curve, and judge the ability of diagnosis by calculating the area under the curve (Area UnderCurve). The value of the area under the ROC curve is between 1.0 and 0.5. In the case of AUC>0.5, the closer the...

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Abstract

The invention relates to a myocardial infarction diagnostic marker composition and in particular relates to application of miR-1468-5p and miR-892b, which are used as molecular marker compositions, to preparation of a myocardial infarction diagnostic reagent. An existing myocardial infarction diagnostic marker is not sufficient to meet the requirements of clinical precision diagnosis and treatment requirements; expression data of myocardial infarction miRNA (micro Ribonucleic Acid) is obtained based on a high-throughput sequencing method and myocardial infarction related miRNA data of a GEO (Gene Expression Omnibus) database center is combined to carry out bioinformatics analysis and verification; the miR-1468-5p and the miR-892b are selected from candidate miRNA and bioinformatics verification is carried out; a result shows that the miR-1468-5p and the miR-892b are closely related to myocardial infarction. The myocardial infarction diagnostic marker composition can be used for clinical diagnosis and prevention and detection of the myocardial infarction in an independent or combined manner.

Description

technical field [0001] The present invention relates to the field of disease diagnosis, in particular to a myocardial infarction diagnostic marker composition, and more specifically to the application of miR-1468-5p and miR-892b as molecular marker compositions in the preparation of myocardial infarction diagnostic reagents. Background technique [0002] Acute myocardial infarction (AMI) is the most serious cardiovascular disease, and early and accurate diagnosis can ensure the immediate initiation of reperfusion therapy and possibly reduce mortality. Some biomarkers such as cardiac troponin I have been applied to diagnose AMI, which can improve the current diagnosis and become a new method for acute myocardial infarction. However, the expression and possible role of miRNAs in acute myocardial infarction are less studied, previous studies have shown that miR-30a is associated with cardiac hypertrophy, miR-195 target genes regulate apoptosis, cell proliferation and cell cycle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 李曙光孙锦云
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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