Method for diagnosing cardiac troponin I (cTn I) in semi-quantitative mode by employing double-indicatrix immunochromatography

Abstract

The invention discloses a detection method in the technical field of biological engineering, in particular a method for diagnosing cardiac troponin I (cTn I) in a semi-quantitative mode by employing double-indicatrix immunochromatography. Acute myocardial infarction (AMI) is a serious disease in a coronary heart disease, is a common emergency among middle-aged and elderly people, and is high in death rate. According to statistics, one third to a half of patients die before being sent to a hospital. In the next 20 years, the AMI is a primary cause for death of the patients around the world. The cTn I is a reliable index which is currently acknowledged and has the highest specificity and the longest duration for myocardial infarction diagnosis. At present, cTn I detection methods which are commonly used in clinic comprise an enzyme-linked immunosorbent assay method and a radioimmunoassay method, which are time-saving, labor-saving, difficult to popularize and high in cost. An immune colloidal gold technology is quickly developed in recent years and can be widely applied to detection of communicable diseases, early pregnancy and cancers. By employing colloidal gold immunochromatography, a quick detection method for the cTn I is established. By adoption of the method, a cTn I gray area of 1 to 5 ng / ml can be subjected to semi-quantitative detection.

Description

Technical field [0001] The present invention involves a detection method in the field of biological engineering technology, which is specifically a method to diagnose Cardioplasma protein i with dual indicator line immune layer. Background technique [0002] With the improvement of the living standards of the people in my country, the incidence of hyperlipidemia, hypertension, and coronary heart disease has also increased significantly.Acute myocardial infarction (AMI) is a very serious type in coronary heart disease. It is a coronary arterial occlusion and blood flow interruption, which causes some myocardial muscle to have local necrosis due to severe and lasting ischemia, which will cause serious heart function.Damage and high mortality rate. According to statistics, about 1 / 3-1 / 2 patients died before being sent to the hospital.Acute myocardial infarction is more common in Europe and the United States, and my country is relatively small, and Beijing and Tianjin and North China...

AI Technical Summary

Problems solved by technology

However, the clinical standard of 1 ng / mL alone cannot directly and quickly distinguish the severity of myocardial infarction, so it is necessary to develop semi-quantitative colloidal gold test paper that can detect cTnI 1-5 ng / mL, the gray area of ​​cTnI diagnosis

At present, there are no relevant test strips for sale at home and abroad

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