Chip device for myocardial infarction diagnosis

Abstract

The invention discloses a chip device for myocardial infarction diagnosis. The chip device is characterized by comprising a sensor main body, wherein a sample injection area, a columnar filtering area, a reagent storage area, a detection area, a micro channel, a magnetic device and an optical detection device are sequentially arranged on the sensor main body along a liquid flow direction; the sample injection area is used for dropwise adding a whole blood sample; the columnar filtering area is used for filtering blood cells and only allows proteins in blood to pass; reagents for a biochemical reaction are arranged in the reagent storage area and comprise a magnetic bead labeled anti-human cTnI monoclonal antibody and a luminescence molecule labeled anti-human cTnI monoclonal antibody; the reagent storage area and the detection area are communicated through the micro channel; the magnetic device is arranged below the sensor main body and is positioned just under the detection area; the optical detection device is arranged above the sensor main body and is positioned just above the detection area. According to the device, quantitative and accurate detection can be realized, the detection time is short, and the device is small in size and portable.

Description

[0001] technical field [0002] The invention relates to the technical field of detecting cardiac troponin I, in particular to a chip device for detecting myocardial infarction marker protein. Background technique [0003] Troponin is composed of three subunits of troponin I, T, and C, and plays an important regulatory role in the process of muscle contraction and relaxation. When the myocardium is injured, the cardiac troponin complex is released into the blood. Among the three subunits, troponin I has a high degree of myocardial specificity and sensitivity, so cardiac troponin I has become the most ideal myocardial infarction landmark. [0004] Routine detection methods for cardiac troponin I include radioimmunoassays (RIAs), high-pressure liquid chromatography, electrochemical analysis, protein analyzers, various diagnostic kits and immunochromatographic biochips, etc. These methods have radioactive contamination , The need for large instruments, time-consuming, quantit...

AI Technical Summary

Problems solved by technology

[0004] Routine detection methods for cardiac troponin I include radioimmunoassays (RIAs), high-pressure liquid chromatography, electrochemical analysis, protein analyzers, various diagnostic kits and immunochromatographic biochips, etc. These methods have radioactive contamination , the need for large instruments, long time-consuming, quantitative detection and other shortcomings, the clinical application has certain limitations, and it is not easy to popularize and use

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