Detection method for rs7832767 locus polymorphism of myocardial-infarction susceptibility gene and detection kit
A site polymorphism, myocardial infarction technology, applied in the fields of molecular biology and medicine, can solve the problems of unproven the correlation between rs7832767 and MI, lack of transmembrane area, etc., to achieve simple diagnosis and treatment, prevention of myocardial infarction, high sensitivity high effect
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Embodiment 1
[0025] Example 1, the detection method of the susceptibility gene for myocardial infarction is to detect the genotype of the rs7832767 site of the SFRP1 gene, and the risk of myocardial infarction in individuals with T genotype in rs7832767 is significantly higher than that of the general population. The rs7832767 is located in intron 1 of SFRP1 (fragment contig contig: NT_167187.1 position 29018005C / T). Wherein, the deoxyribonucleic acid (DNA) sequence number: rs7832767 position is based on SEQ ID NO:1. The study found that the SFRP1 gene is mainly expressed in aortic endothelium, heart, spleen, eyes, blood vessels and other tissues, and higher SFRP1 levels may be detected during heart maturation and in adult cardiomyocytes.
Embodiment 2
[0026] Example 2, as an optimization of the above example, the rs7832767 site is located in intron 1 of the SFRP1 gene, and the nucleotide sequence of the SFRP1 gene has the sequence shown in SEQ ID NO:1.
Embodiment 3
[0027] Embodiment 3, as the optimization of the above-mentioned embodiment, the detection method of the susceptibility gene of myocardial infarction is carried out according to the following steps: the first step, collect the blood sample sample according to the 2ml blood sample sample collected in each case of the person to be tested, extract from the blood sample sample Genomic DNA 50ng as template, add 2×PowerTaqPCRMasterMix 25μl, 10μmol / L forward primer and reverse primer 1μl each, then add sterilized ultrapure water to make up 50μl, pre-denature at 95°C for 5min, and then carry out 35 cycles of amplification as follows : Denaturation at 95°C for 30s, annealing at 51°C for 30s, extension at 72°C for 45s, and finally extension at 72°C for 10 minutes to obtain PCR amplification products after amplification; in the second step, take 10 μl of PCR amplification products and add 1 unit of endonuclease, enzyme 1 μl of cutting buffer and 9 μl of sterilized ultrapure water were used...
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