Detection method for rs7832767 locus polymorphism of myocardial-infarction susceptibility gene and detection kit

A site polymorphism, myocardial infarction technology, applied in the fields of molecular biology and medicine, can solve the problems of unproven the correlation between rs7832767 and MI, lack of transmembrane area, etc., to achieve simple diagnosis and treatment, prevention of myocardial infarction, high sensitivity high effect

Inactive Publication Date: 2016-07-13
THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] , that is, soluble frizzled related protein-1 (soluble frizzled related protein-l), which has a similar cysteine-rich secretory protein as the frizzled receptor in the Wnt signaling pathway, but cannot bind to the plasma membrane due to the lack of a transmembrane region , is considered to bind to circulating Wnt in the interstitium, thus preventing Wnt protein from binding to the frizzled receptor on the plasma membrane, negatively regulating the Wnt signaling pathway, and inhibiting the excessive activation of the Wnt signaling pathway, but the current SFRP1 and MI Few studies have confirmed the report of rs7832767 association with MI

Method used

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  • Detection method for rs7832767 locus polymorphism of myocardial-infarction susceptibility gene and detection kit
  • Detection method for rs7832767 locus polymorphism of myocardial-infarction susceptibility gene and detection kit

Examples

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Embodiment 1

[0025] Example 1, the detection method of the susceptibility gene for myocardial infarction is to detect the genotype of the rs7832767 site of the SFRP1 gene, and the risk of myocardial infarction in individuals with T genotype in rs7832767 is significantly higher than that of the general population. The rs7832767 is located in intron 1 of SFRP1 (fragment contig contig: NT_167187.1 position 29018005C / T). Wherein, the deoxyribonucleic acid (DNA) sequence number: rs7832767 position is based on SEQ ID NO:1. The study found that the SFRP1 gene is mainly expressed in aortic endothelium, heart, spleen, eyes, blood vessels and other tissues, and higher SFRP1 levels may be detected during heart maturation and in adult cardiomyocytes.

Embodiment 2

[0026] Example 2, as an optimization of the above example, the rs7832767 site is located in intron 1 of the SFRP1 gene, and the nucleotide sequence of the SFRP1 gene has the sequence shown in SEQ ID NO:1.

Embodiment 3

[0027] Embodiment 3, as the optimization of the above-mentioned embodiment, the detection method of the susceptibility gene of myocardial infarction is carried out according to the following steps: the first step, collect the blood sample sample according to the 2ml blood sample sample collected in each case of the person to be tested, extract from the blood sample sample Genomic DNA 50ng as template, add 2×PowerTaqPCRMasterMix 25μl, 10μmol / L forward primer and reverse primer 1μl each, then add sterilized ultrapure water to make up 50μl, pre-denature at 95°C for 5min, and then carry out 35 cycles of amplification as follows : Denaturation at 95°C for 30s, annealing at 51°C for 30s, extension at 72°C for 45s, and finally extension at 72°C for 10 minutes to obtain PCR amplification products after amplification; in the second step, take 10 μl of PCR amplification products and add 1 unit of endonuclease, enzyme 1 μl of cutting buffer and 9 μl of sterilized ultrapure water were used...

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Abstract

The invention relates to the technical field of molecular biology and medicine, and discloses a detection method for the rs7832767 locus polymorphism of a myocardial-infarction susceptibility gene and a detection kit.The detection method includes the steps that the genotype of an rs7832767 locus of an SFRP1 gene is detected, wherein the myocardial infarction onset risk of persons with the T genotype in rs7832767 is remarkably higher than that of ordinary persons.The invention further discloses the corresponding detection kit.The detection kit comprises a primer for amplifying the rs7832767 locus.The genotype of the rs7832767 locus is detected with the detection method, persons with the potential myocardial infarction onset risk are screened out, and the aim of preventing myocardial infarction is achieved; the detection method and the detection kit are easy and convenient to operate, short in detection time, low in cost and high in sensitivity and specificity, and a simple and direct new path is provided for diagnosing and treating myocardial infarction.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and medicine, and relates to a detection method and a detection kit for the rs7832767 site polymorphism of a myocardial infarction susceptibility gene. Background technique [0002] Myocardial infarction (MI) is a serious state of coronary atherosclerotic heart disease (coronary artery disease), and its morbidity and mortality rank first among coronary heart diseases. Epidemiological and clinical studies have shown that with the prolongation of life expectancy, the affluence of material conditions and the westernization of cultural lifestyles, cardiovascular diseases, especially the prevalence and mortality of myocardial infarction in many developing countries (including China) are increasing. The rising trend has attracted worldwide attention. It is estimated that by 2020, myocardial infarction will become the number one disease in the world. Although the diagnosis and treatment of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 马依彤陶静陈邦党刘芬王永涛玛依拉·阿布都克力木贺春晖李华垠
Owner THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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