The invention relates to the technical field of virus sample preservation, in particular to a virus preserving fluid capable of effectively preserving samples such as virus for a long time. The preserving fluid comprises the following components: disodium hydrogen phosphate dodecahydrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, potassium chloride, magnesiumchloride hexahydrate, calcium chloride, double distilled water, glucose, antibiotics, sodium bicarbonate, sodium hydroxide, a stabilizer, amino acid, phenol red, a biological buffering agent, a cryoprotectant and glycerol, wherein the components comprise, by mass, 0.06-3.04 g of disodium hydrogen phosphate dodecahydrate, 0.06-1.2 g of potassium dihydrogen phosphate, 0.20-2.0 g of magnesium sulfate heptahydrate, 150-160 g of sodium chloride, 0.70-8.0 g of potassium chloride, 0.30-2.0 g of magnesium chloride hexahydrate, and 0.14-2.8 g of calcium chloride, 20.0 g of glucose, 0.50-5.5 g of antibiotics, 0.35-0.60 g of sodium bicarbonate, 10 ml of sodium hydroxide, 0.10-1.0 g of stabilizer, 0.30-3.0 g of amino acid and 0.30-0.40 g of phenol red. The preserving fluid can protect virus protein shells from being decomposed easily; and the primitiveness of the virus sample is kept to the maximum extent, so that the preservation integrity of the virus is better, and the detection rate is higher.