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Homocysteine detecting kit and using method thereof

A technology for homocysteine ​​and detection kits, which is applied in the field of kits for determining chemical and physical properties of materials, can solve the problems of reagent stability, unfavorable reagent long-term storage, waste, etc., to improve stability and accuracy Sex, the effect of promoting the reaction system

Inactive Publication Date: 2017-10-20
WHITMAN BIOTECH NANJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of various enzymes in the common homocysteine ​​detection reagent, the stability of the reagent will be affected, which is not conducive to the long-term storage of the reagent, resulting in adverse consequences of waste

Method used

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  • Homocysteine detecting kit and using method thereof
  • Homocysteine detecting kit and using method thereof
  • Homocysteine detecting kit and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The raw materials and proportions of the homocysteine ​​detection kit of this embodiment are as follows:

[0049] Reagent 1 includes:

[0050]

[0051] Reagent 2 includes:

[0052]

[0053]Among them, cystathionine-β-synthase and methionine synthase have the same specific activity, and cystathionine-β-lyase and methionine-γ-lyase have the same specific activity.

Embodiment 2

[0055] The raw materials and proportions of the homocysteine ​​detection kit of this embodiment are as follows:

[0056] Reagent 1 includes:

[0057]

[0058] Reagent 2 includes:

[0059]

[0060] Among them, cystathionine-β-synthase and methionine synthase have the same specific activity, and cystathionine-β-lyase and methionine-γ-lyase have the same specific activity.

Embodiment 3

[0062] The raw materials and proportions of the homocysteine ​​detection kit of this embodiment are as follows:

[0063] Reagent 1 includes:

[0064]

[0065] Reagent 2 includes:

[0066]

[0067] Homocysteine ​​detection kit calibration curve of the present invention is as follows image 3 Shown; Homocysteine ​​detection kit calibration reaction curve of the present invention is as Figure 4 shown.

[0068] Among them, cystathionine-β-synthase and methionine synthase have the same specific activity, and cystathionine-β-lyase and methionine-γ-lyase have the same specific activity.

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Abstract

The invention provides a homocysteine detecting kit and a using method thereof, relates to the field of biochemical detection, and aims to provide a homocysteine detecting kit which is high in stability, high in accuracy and high in precision and a using method of the homocysteine detecting kit. The homocysteine detecting kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a capso buffer solution, lactic dehydrogenase (LDH), L-serine, reduced coenzyme Thio-NADH, tris((2-carboxyethyl)phosphine, mercaptoethanol, an alkylphenol polyoxyethylene series, EDTA, polyethylene glycol 300, ascorbic acid oxidase, a composite stabilizer, and a proclin series preservative; and the reagent 2 comprises a capso buffer solution, cystathionine-beta-synthase, methionine synthase, cystathionine-beta-lyase, methionine-gamma lyase, a surfactant and sodium dodecylbenzene sulfonate. By the various added mixed enzymes and methionine-gamma lyases, the accuracy of the kit on detection of homocysteine can be improved.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a kit for measuring materials by means of measuring their chemical and physical properties. Background technique [0002] Homocysteine ​​(also known as homocysteine, homocysteine, Hcy) is an intermediate product in the process of methionine metabolism. The catabolism of homocysteine ​​in the body is most affected by two factors. One is the lack of certain biological enzymes related to congenital inheritance, and the other is the lack of nutrients such as folic acid, vitamin B6, and vitamin B12 in the body. Both of these deficiencies affect the conversion of homocysteine ​​to other substances, resulting in hyperhomocysteinemia. The determination of Hcy has important clinical significance in the judgment of cardiovascular disease, uremia, atherosclerosis, diabetes, Alzheimer's disease and other diseases. [0003] About 70% of homocysteine ​​in plasma is combined with albumin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6815
Inventor 常俊骏孙文勇郑佳
Owner WHITMAN BIOTECH NANJING
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