Virus preserving fluid capable of effectively preserving virus and other samples for long time

Abstract

The invention relates to the technical field of virus sample preservation, in particular to a virus preserving fluid capable of effectively preserving samples such as virus for a long time. The preserving fluid comprises the following components: disodium hydrogen phosphate dodecahydrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, potassium chloride, magnesiumchloride hexahydrate, calcium chloride, double distilled water, glucose, antibiotics, sodium bicarbonate, sodium hydroxide, a stabilizer, amino acid, phenol red, a biological buffering agent, a cryoprotectant and glycerol, wherein the components comprise, by mass, 0.06-3.04 g of disodium hydrogen phosphate dodecahydrate, 0.06-1.2 g of potassium dihydrogen phosphate, 0.20-2.0 g of magnesium sulfate heptahydrate, 150-160 g of sodium chloride, 0.70-8.0 g of potassium chloride, 0.30-2.0 g of magnesium chloride hexahydrate, and 0.14-2.8 g of calcium chloride, 20.0 g of glucose, 0.50-5.5 g of antibiotics, 0.35-0.60 g of sodium bicarbonate, 10 ml of sodium hydroxide, 0.10-1.0 g of stabilizer, 0.30-3.0 g of amino acid and 0.30-0.40 g of phenol red. The preserving fluid can protect virus protein shells from being decomposed easily; and the primitiveness of the virus sample is kept to the maximum extent, so that the preservation integrity of the virus is better, and the detection rate is higher.

Description

technical field

[0001] The invention relates to the technical field of virus sample preservation, in particular to a virus preservation solution capable of effectively preserving viruses and other samples for a long time. Background technique

[0002] According to statistics, there are 600 million to 1.2 billion cases of influenza in the world every year, of which 3 million to 5 million are severe influenza, 250,000 to 500,000 people die, and the fatality rate of severe influenza can reach 8%. Avian influenza is an acute respiratory infectious disease caused by some strains of certain subtypes of avian influenza virus. Among them, the human highly pathogenic avian influenza virus (H7N9) is likely to cause severe pneumonia with a high case fatality rate, which is harmful to people's body and mind. The health hazard is great. Hank's solution is a common balanced salt solution (BalancedSaltSolution, BSS) for the delivery and preservation of influenza virus. Affected by variou...

Examples

Embodiment 1

[0026] Embodiment 1: 150~160g of sodium chloride, 0.20~2.0g of magnesium sulfate heptahydrate, 0.70~8.0g of potassium chloride, 0.30~2.0g of magnesium chloride hexahydrate, and 0.14~2.8g of calcium chloride are dissolved in 1000ml double distilled water Obtain stock solution A; dissolve 0.06-3.04 g of disodium hydrogen phosphate dodecahydrate, 0.06-1.2 g of potassium dihydrogen phosphate, and 20.0 g of glucose in 800 ml of double-distilled water to obtain solution (1); then take phenol red phenol red 0.30- .040g, put in a glass mortar, add sodium hydroxide drop by drop, and grind until completely dissolved, add about 10ml of sodium hydroxide, suck the dissolved phenol red into a 100ml measuring bottle, wash the mortar with double distilled water The phenol red solution remaining in the solution was put into a measuring bottle, and finally double distilled water was added to 100ml to obtain solution (2); solution (1) was mixed with solution (2), and double distilled water was ad...

Embodiment 2

[0027]Embodiment 2: 150~160g of sodium chloride, 0.20~2.0g of magnesium sulfate heptahydrate, 0.70~8.0g of potassium chloride, 0.30~2.0g of magnesium chloride hexahydrate, and 0.14~2.8g of calcium chloride are dissolved in 1000ml double distilled water Obtain stock solution A; dissolve 0.06-3.04 g of disodium hydrogen phosphate dodecahydrate, 0.06-1.2 g of potassium dihydrogen phosphate, and 20.0 g of glucose in 800 ml of double-distilled water to obtain solution (1); then take phenol red phenol red 0.30- .040g, put in a glass mortar, add sodium hydroxide drop by drop, and grind until completely dissolved, add about 10ml of sodium hydroxide, suck the dissolved phenol red into a 100ml measuring bottle, wash the mortar with double distilled water The phenol red solution remaining in the solution was put into a measuring bottle, and finally double distilled water was added to 100ml to obtain solution (2); solution (1) was mixed with solution (2), and double distilled water was add...

technical field

[0001] The invention relates to the technical field of virus sample preservation, in particular to a virus preservation solution capable of effectively preserving viruses and other samples for a long time. Background technique

[0002] According to statistics, there are 600 million to 1.2 billion cases of influenza in the world every year, of which 3 million to 5 million are severe influenza, 250,000 to 500,000 people die, and the fatality rate of severe influenza can reach 8%. Avian influenza is an acute respiratory infectious disease caused by some strains of certain subtypes of avian influenza virus. Among them, the human highly pathogenic avian influenza virus (H7N9) is likely to cause severe pneumonia with a high case fatality rate, which is harmful to people's body and mind. The health hazard is great. Hank's solution is a common balanced salt solution (BalancedSaltSolution, BSS) for the delivery and preservation of influenza virus. Affected by variou...

Examples

Embodiment 1

[0026] Embodiment 1: 150~160g of sodium chloride, 0.20~2.0g of magnesium sulfate heptahydrate, 0.70~8.0g of potassium chloride, 0.30~2.0g of magnesium chloride hexahydrate, and 0.14~2.8g of calcium chloride are dissolved in 1000ml double distilled water Obtain stock solution A; dissolve 0.06-3.04 g of disodium hydrogen phosphate dodecahydrate, 0.06-1.2 g of potassium dihydrogen phosphate, and 20.0 g of glucose in 800 ml of double-distilled water to obtain solution (1); then take phenol red phenol red 0.30- .040g, put in a glass mortar, add sodium hydroxide drop by drop, and grind until completely dissolved, add about 10ml of sodium hydroxide, suck the dissolved phenol red into a 100ml measuring bottle, wash the mortar with double distilled water The phenol red solution remaining in the solution was put into a measuring bottle, and finally double distilled water was added to 100ml to obtain solution (2); solution (1) was mixed with solution (2), and double distilled water was ad...

Embodiment 2

[0027]Embodiment 2: 150~160g of sodium chloride, 0.20~2.0g of magnesium sulfate heptahydrate, 0.70~8.0g of potassium chloride, 0.30~2.0g of magnesium chloride hexahydrate, and 0.14~2.8g of calcium chloride are dissolved in 1000ml double distilled water Obtain stock solution A; dissolve 0.06-3.04 g of disodium hydrogen phosphate dodecahydrate, 0.06-1.2 g of potassium dihydrogen phosphate, and 20.0 g of glucose in 800 ml of double-distilled water to obtain solution (1); then take phenol red phenol red 0.30- .040g, put in a glass mortar, add sodium hydroxide drop by drop, and grind until completely dissolved, add about 10ml of sodium hydroxide, suck the dissolved phenol red into a 100ml measuring bottle, wash the mortar with double distilled water The phenol red solution remaining in the solution was put into a measuring bottle, and finally double distilled water was added to 100ml to obtain solution (2); solution (1) was mixed with solution (2), and double distilled water was add...