Virus preserving fluid capable of effectively preserving virus and other samples for long time

Abstract

The invention relates to the technical field of virus sample preservation, in particular to a virus preserving fluid capable of effectively preserving samples such as virus for a long time. The preserving fluid comprises the following components: disodium hydrogen phosphate dodecahydrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, potassium chloride, magnesiumchloride hexahydrate, calcium chloride, double distilled water, glucose, antibiotics, sodium bicarbonate, sodium hydroxide, a stabilizer, amino acid, phenol red, a biological buffering agent, a cryoprotectant and glycerol, wherein the components comprise, by mass, 0.06-3.04 g of disodium hydrogen phosphate dodecahydrate, 0.06-1.2 g of potassium dihydrogen phosphate, 0.20-2.0 g of magnesium sulfate heptahydrate, 150-160 g of sodium chloride, 0.70-8.0 g of potassium chloride, 0.30-2.0 g of magnesium chloride hexahydrate, and 0.14-2.8 g of calcium chloride, 20.0 g of glucose, 0.50-5.5 g of antibiotics, 0.35-0.60 g of sodium bicarbonate, 10 ml of sodium hydroxide, 0.10-1.0 g of stabilizer, 0.30-3.0 g of amino acid and 0.30-0.40 g of phenol red. The preserving fluid can protect virus protein shells from being decomposed easily; and the primitiveness of the virus sample is kept to the maximum extent, so that the preservation integrity of the virus is better, and the detection rate is higher.

Description

technical field [0001] The invention relates to the technical field of virus sample preservation, in particular to a virus preservation solution capable of effectively preserving viruses and other samples for a long time. Background technique [0002] According to statistics, there are 600 million to 1.2 billion cases of influenza in the world every year, of which 3 million to 5 million are severe influenza, 250,000 to 500,000 people die, and the fatality rate of severe influenza can reach 8%. Avian influenza is an acute respiratory infectious disease caused by some strains of certain subtypes of avian influenza virus. Among them, the human highly pathogenic avian influenza virus (H7N9) is likely to cause severe pneumonia with a high case fatality rate, which is harmful to people's body and mind. The health hazard is great. Hank's solution is a common balanced salt solution (BalancedSaltSolution, BSS) for the delivery and preservation of influenza virus. Affected by variou...

AI Technical Summary

Problems solved by technology

Affected by various factors, influenza virus stored in Hank's solution can only be stored for a few hours. After several hours, the integrity of the virus form will be affected, which in turn will affect the virus extraction rate. If the time is prolonged, it will be very difficult. It is easy to breed bacteria and fungi, leading to a decrease in the pH value of Hank's solution. When the pH value of Hank's solution is less than 7.4, the stability of the virus decreases and the decomposition speed of the virus is accelerated, so it is difficult to preserve the virus for a long time. Therefore, a long-term effective preservation method is proposed. Virus preservation solution for samples such as viruses