60results about How to "No effect on activity" patented technology

The invention relates to a natural pharmaceutical composition, a preparation method thereof and an application of the natural pharmaceutical composition in preparation of attenuated synergistic medicaments in cancer radiotherapy and chemotherapy. The natural pharmaceutical composition is prepared from extract obtained by decocting and concentrating ginger and dried orange peel decoction, gingerol, dried orange peel water extract and nobiletin according to the ratios of (3-5):(2-9):(1-6):(2-5). The composition has an excellent effect on treating emesis and other side effects caused by cancer radiotherapy and chemotherapy, and can simultaneously play a role in inhibiting tumors.

The invention provides a chemical-biological combining method used for repairing Cd pollution in soil. The method comprises the following steps: compounding a citric acid solution 0.6mol/L, a Beta-cyclodextrin solution 20g/L and a rhamnolipid solution 10g/L, or compounding any two of the solutions; adjusting the pH value ranging from 3 to 6 through a nitric acid solution or a sodium hydroxide solution to acquire a leachate a and a leachate b. Combined with chemical elution and phytoremediation, the chemical-biological combining method used for repairing the Cd pollution in the soil repairs the soil polluted by Cd, and has an obvious eliminating effect on the exchangeable form of the Cdi in the soil and the combining form of carbonate, particularly the combining form of iron and manganese oxides; the repairing method can be applied to repairing large-scale polluted sites in city; the engineering quantity is small, the cost is low and the period is short; moreover, the environment of the city can be beautified when the environment is repaired, and no secondary pollution is generated to the soil; besides, the chemical-biological combining method does not have an effect on the activity of the soil, and the repairing rate can reaches above 92%.

The invention relates to a natural pharmaceutical composition, a preparation method of the natural pharmaceutical composition and the use of the natural pharmaceutical composition for preparing toxicity-reducing efficacy-improving medicine in cancer radiotherapy and chemotherapy. The natural pharmaceutical composition consists of decocted and concentrated extract of ginger and elecampane, gingerol, elecampane chloroform extract and elecampane hexane extract based on the proportion of 4 to 8: 3 to 8: 4 to 5: 2 to 4, and the composition has extremely good therapeutic effect for vomiting and other side effects caused by cancer radiotherapy and chemotherapy and can simultaneously play the role of inhibiting tumor.

The invention provides a method for preparing a solid enzyme reagent which is used to fast measure the organophosphorus and carbamate toxicity of pesticide residue and its using method. The agent is formed by choline esterase, buffer solution and carrier. The solid enzyme reagent can be stored at indoor temperature above 20 days.

The present invention relates to a modified polyacrylamide immobilized cell preparation method, comprises the following steps: 1) modified polyacrylamide synthesis: using ammonium persulfate as initiator, formate solium as chain transferring agent for synthesizing low molecular weight polyacrylamide, hydroxymethylating polyacrylamide using formaldehyde, condensating it with acrylamide to obtain modified polyacrylamide 2) immobilized microbe cell preparation: mixing modified polyacrylamide aqueous solution and prepared bacteria suspension liquid, adding crosslinking agent and initiator, then adding catalyst tetramethylethylenediamine and mixing evenly, keeping still for several minutes to obtain immobilized cell gelatin.The coagulation speed of gelatin is quick under room temperature. Low coagulation temperature can keep embedment microbe activity. The prepared immobilized microbe cell gelatin can be used in alive cell and enzyme immobilization.

The invention relates to a method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot. The method comprises the steps of dipping the fruit or pomace of the aronia melanocarpa elliot into an organic solvent, conducting Flash extraction for 0.5-30 minutes by using a Flash extractor, carrying out suction filtration and centrifugation to obtain a procyanidine extract solution of the aronia melanocarpa elliot; concentrating the procyanidine extract solution of the aronia melanocarpa elliot by using a negative pressure rotary evaporation device to obtain procyanidine extract concrete of the aronia melanocarpa elliot; and after pre-freezing the procyanidine extract concrete of the aronia melanocarpa elliot by using an ultra-low temperature refrigerator, carrying out freeze drying on the procyanidine extract concrete by using a freeze drying device to obtain the freeze-dried powder of a procyanidine extract of the aronia melanocarpa elliot. According to the method disclosed by the invention, low temperature in the whole course can be realized, procyanidine disintegration caused by high temperature can be avoided, the loss of anthocyanin in the extracting process effectively can be effectively avoided, the operation is simple and the cost is low, and promotion in enterprises can be achieved by forming an automatic production line, so that the method has a relatively high commercial value.

The invention relates to a natural pharmaceutical composition, a preparation method of the natural pharmaceutical composition, and an application of the natural pharmaceutical composition in preparation of attenuated synergistic medicines for cancer chemotherapy. The natural pharmaceutical composition is prepared from reed rhizome extract and gingerol according to the ratio of (3-24):1. The composition has an excellent curative effect on side effects such as vomiting and the like caused by cancer chemotherapy, and can play a role in inhibiting tumors.

The invention provides a homogeneous immunodetection kit for detecting an anti-cyclic citrullinated peptide antibody. The kit is based on a double antigen sandwich reaction mode and prepared by adopting high sensitivity and specificity of cyclic citrullinated peptide-coupled carrier protein as an antigen raw material; and the kit can detect all types of anti-citrulline antibodies and has the advantages of being strong in specificity, good in signal amplification effect, high in sensitivity, wide in linearity range, simple in operation, good in stability and high in precision.

The invention relates to a homogeneous immunoassay kit for detecting target anti-Carp antibodies and application thereof, wherein the kit adopts a one-step competition reaction mode, and the reactionmode can specifically detect the anti-Carp Ab such as IgG, IgA and IgM, thereby omitting the process of washing in a conventional indirect method, forming a one-step process to complete the entire reaction. Compared with the traditional indirect method, the homogeneous immunoassay kit is time-saving, washing-free, and easy to operate.

The invention belongs to the technical field of marine water toxicity monitoring and discloses marine water toxicity biological monitoring equipment based on a binocular vision technology. The equipment comprises a binocular camera holder, a binocular camera set installed on the binocular camera holder, a mussel activity observation system, a slide rail for connecting the camera holder and the mussel activity observation system, a visual signal processing operation circuit, a watertight cable for connecting the binocular camera holder and the visual signal processing operation circuit, and a computer which is connected to the visual signal processing operation circuit and can send out instructions and output results. The equipment has a simple structure, is convenient to replace mussel living bodies, has high resolution and high measurement accuracy, has no influence on activity of living organisms, and has high reliability.

The invention relates to a w/o/w adjuvant composition. The w/o/w adjuvant composition comprises a sucrose fatty acid ester with HLB value of below 5, fatty alcohol-polyoxyethylene ether and oil. The vaccine composition prepared by the adjuvant composition is stable at different temperature and has no layering and demulsification phenomena, and the preparation condition of the vaccine is simple andmild. The prepared vaccine achieves the immune effect rapidly and does not have adverse effect during use.

The invention discloses a lead ion fast visual detection method. The method comprises: performing hybrid hybridization of fundamental chains and enzyme chains to form a GR-5 DNA enzyme; adding oxidized graphene for treatment to remove unreacted free single-stranded nucleic acid to eliminate the false positive effect; spraying two kinds of gold label probes on a gold label mat; and adding two chains which are obtained from the GR-5 DNA enzyme which is a pretreated to-be-detected sample through cutting at the rA position, wherein the two chains together with the gold label probes and nucleotide sequences located on a detection line form a sandwich structure through chromatography, so that the chains are fixed at the detection line for color development, and excessive free nucleic acid is fixed at a quality control line for color development. According to the intensity of color development on the detection line, the lead ion concentration is obtained. Lead ions can be detected in 15 min at the room temperature, and the minimum limit of detection of the lead ions can reach 0.05 nM. The method is high in sensitivity and good in specificity, is not affected by other divalent metal ions, is simple and easy to operate, and can be directly applied to detection of lead ions in the environment.

The invention provides an attenuated salmonella typhimurium mediated eukaryocyte plasmid transfection method. According to the transfection method, based on an intracellular invasion characteristic of the attenuated salmonella typhimurium, recombinant plasmids are carried to enter eukaryocytes to realize specific protein expression. Specifically, recombinant eukaryotic expression plasmids are introduced into an attenuated salmonella typhimurium VNP20009 strain in an electric shock transformation way; then recombinant bacteria and the eukaryocytes are cultured in a co-incubation manner; and finally, the attenuated salmonella typhimurium inside and outside cells is killed by a serum culture medium containing penicillin and streptomycin, and the recombinant plasmids are released to the eukaryocytes to realize the specific protein expression. Compared with conventional calcium sulfate transfection, liposome transfection and electro-transformation, the transfection method disclosed by the invention takes viable bacteria as a bearing host of specific plasmids, so that a larger number of target vectors can be obtained by only conventional culture; and in addition, the plasmid transfection operating steps are simplified, the cost is lowered, and the transfection efficiency and the cytotoxicity are comprehensively better than those in the conventional transfection method.

The invention discloses a method for producing high fructose syrup by immobilized glucose isomerase. The method comprises the following steps: immobilizing glucose isomerase in an ultra-filtration membrane with an asymmetric structure in a filtering mode to prepare a membrane bioreactor of the immobilized glucose isomerase; performing reaction on the glucose solution in batches or continuously by utilizing the membrane bioreactor to produce the high fructose syrup. According to the method, an enzyme catalysis process and a membrane separating process are integrated, so that the production process is simplified, and the activity and the stability of an enzyme are improved; meanwhile, an enzyme membrane reactor can be used repeatedly, solvent cleaning and batchwise feeding are avoided, the development direction of green chemistry is met, continuous production is realized, the production efficiency is improved, and reference is provided for industrial production of fructose.

The invention relates to a separation and purification method of umbilical cord mesenchymal stem cells and belongs to the technical field of stem cells and regenerative medicine. The method comprisesthe steps as follows: separation of umbilical cord tissue: taking the umbilical cord tissue, cleaning the umbilical cord tissue and removing blood, and shearing the tissue into tissue fragments for later use; tissue digestion: taking the tissue fragments, and adding tissue digestive juice for oscillation digestion, wherein the tissue digestive juice contains neutral protease, hyaluronidase, collagenase II and DNA enzyme; after digestion, adding a stop buffer for stop, performing filtration to obtain undigested tissue blocks and filtrate, and centrifuging the filtrate to remove supernatant to obtain cells; and cell culture: inoculating a culture flask with the tissue blocks and cells, using serum-free culture medium for culture to obtain the umbilical cord mesenchymal stem cells. The separation and purification method of umbilical cord mesenchymal stem cells overcomes the defects of an enzyme digestion method and a tissue block culture method in the prior art and can obtain enough primary cells within 3-5 days.

The present invention relates to an immunity enhancing health product and a preparation method thereof, wherein the raw materials comprise, by weight, 230-250 parts of cervus elaphus linnaeus, 6-18 parts of a ginseng ethanol extract, 5-15 parts of a ginseng residue water extract, and 28-48 parts of an ophiopogon japonicus water extract. The preparation method comprises: (1) crushing ginseng into coarse grains, carrying out reflux extraction with ethanol, filtering, concentrating the filtrate, carrying out vacuum drying, and crushing so as to be spare; (2) adding water to the residue obtained in the step (1), cooking, filtering, concentrating the filtrate, carrying out vacuum drying, and crushing so as to be spare; (3) crushing ophiopogon japonicus into coarse grains, adding water, cooking, filtering, concentrating the filtrate, carrying out vacuum drying, and crushing so as to be spare; (4) removing villus from cervus elaphus linnaeus in a burning manner, completely scraping, splitting into broken blocks, drying, placing to achieve a cold state, crushing into fine powder, and sterilizing so as to be spare; and (5) weighing the raw materials according to a certain weight part ratio, uniformly mixing, and tableting or loading into capsules. The immunity enhancing health product of the present invention has advantages of simple formula and human body immunity improving.

The invention relates to the technical field of biological medicines, in particular to application of liriope spicata saponin B in preparation of a medicine for treating skin inflammation. The research finds that various inflammatory cytokines can induce skin cell inflammatory reaction, so that the levels of CCL5, CCL17 and CCL22 are significantly increased. HaCaT cells are treated by using the liriope spicata saponin B, so that the levels of CCL5, CCL17 and CCL22 can be reduced, the inflammatory reaction induced by combination of the inflammatory cytokines can be inhibited, and a new way is provided for research and development of a new medicine for resisting the skin inflammation induced by various inflammatory cytokines.

The invention relates to a natural medicine composition, a preparation method thereof and application of the medicine composition in medicines used for preventing and treating nausea and vomiting caused by cancer chemotherapy and having a synergistic effect on cancer treatment. The natural medicine composition is composed of a pepper extract and procyanidins, wherein the mass ratio of the pepper extract to procyanidins is 1:(1-9). The natural medicine composition provided by the invention is an innovative natural medicine composition in fit with a polyceptor target proportion, an experiment result shows that the natural medicine composition can be used for preventing and treating nausea and vomiting, comprising acute vomiting and delayed vomiting, caused by cancer chemotherapy and meanwhile, has the effect of assisting tumor inhibition.

The invention relates to a natural pharmaceutical composition, a preparation method thereof and an application of the natural pharmaceutical composition in preparation of attenuated synergistic medicaments in cancer radiotherapy and chemotherapy. The natural pharmaceutical composition is prepared from extract obtained by decocting and concentrating fingered citron and ginger decoction, gingerol, fingered citron water extract and fingered citron polysaccharide according to the ratios of (3-8):(2-4):(2-3):(2-4). The composition has an excellent effect on treating emesis and other side effects caused by cancer radiotherapy and chemotherapy, and can simultaneously play a role in inhibiting tumors.

The invention relates to a disinfectant for silkworm pathogenic microorganisms and application of the disinfectant, nano titanium dioxide is used as a main agent, alkaline sodium salt is used as an auxiliary agent, and when water is used as a solvent to prepare a solution, the mass volume concentration of the nano titanium dioxide is 1mg/L-100mg/L, and the mass percent concentration of the auxiliary agent is 0.1%-1%. The alkaline sodium salt is any one of trisodium phosphate, chlorinated trisodium phosphate or sodium bicarbonate. More preferably, the mass volume concentration of the nano titanium dioxide is 20 mg/L-50 mg/L, and the mass percent concentration of the alkaline sodium salt is 0.45%-0.5%. A new application of the existing drug nano titanium dioxide is developed, and the disinfectant is safe and reliable as a silkworm drug, has a good killing effect on main pathogens BmNPV, BmCPV, bombyx mori microsporidia, beauveria bassiana, aspergillus flavus and bacillus thuringiensis spores of bombyx mori, and is an efficient broad-spectrum disinfectant for silkworm breeding.

The invention relates to the technical field of biological medicines, in particular to application of complanatoside in preparation of a medicine for treating skin inflammation. The research finds that various inflammatory cytokines can induce skin cell inflammatory response, so that the levels of IL-1beta, ROS and CCL22 are obviously increased; and the levels of IL-1beta, ROS and CCL22 can be reduced by treating HaCaT cells with the complanatoside, the inflammatory reaction induced by the combination of inflammatory cytokines can be inhibited, and a new way is provided for research and development of new drugs for resisting skin inflammation induced by various inflammatory cytokines.