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W/o/w adjuvant composition, vaccine composition prepared thereby, and preparation method of vaccine composition

A vaccine composition and composition technology, applied in biochemical equipment and methods, vaccines, veterinary vaccines, etc., can solve the problems of easy inactivation of antigens, poor stability, high viscosity, etc., and achieve the effect of ensuring the immune effect

Active Publication Date: 2018-01-19
LUOYANG SEIWEI BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Cosmetics in the prior art also use water-in-oil-in-water formulations, but their emulsification preparation usually requires a higher emulsification temperature, which usually exceeds 37 degrees. If this technology is used to prepare vaccines, the antigen is easily inactivated under this condition
[0006] Patent application CN106511995A discloses a biphasic adjuvant, patent application CN106267184A discloses a water-in-oil-in-water emulsifier, and patent application CN103223164A discloses a water-in-oil-in-water type adjuvant vaccine. The problem is that the particle size of the prepared emulsion is relatively large, the viscosity is relatively large, and the stability is poor

Method used

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  • W/o/w adjuvant composition, vaccine composition prepared thereby, and preparation method of vaccine composition
  • W/o/w adjuvant composition, vaccine composition prepared thereby, and preparation method of vaccine composition
  • W/o/w adjuvant composition, vaccine composition prepared thereby, and preparation method of vaccine composition

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Embodiment approach

[0038] As an embodiment of the present invention, the w / o / w adjuvant composition of the above composition further comprises water.

[0039] As an embodiment of the present invention, in the w / o / w adjuvant composition of the present invention, the w / o / w adjuvant composition further includes 0.5%W / W-3% by weight w / w water.

[0040] 1.0% W / W, 1,5% W / W, 2.0% W / W water may also be included in the w / o / w adjuvant composition of the present invention.

[0041] After the above-mentioned content of water is added to the w / o / w adjuvant composition of the present invention, the cold stability of the adjuvant composition is greatly improved.

[0042] As an embodiment of the present invention, in the w / o / w adjuvant composition of the present invention, the oil includes mineral oil, vegetable oil or synthetic metabolizable oil.

[0043] As a preferred embodiment of the present invention, in the w / o / w adjuvant composition of the present invention, the mineral oil is white oil for veterinary...

Embodiment 1

[0071] Embodiment 1, the preparation of adjuvant composition

[0072] Sucrose ester L195 (Mitsubishi Ryoto) 4.0g, AEO-5 4.0g, mineral oil 92g

[0073] Preparation method: Mix sucrose ester L195, AEO-5 and mineral oil evenly, and heat to 30°C.

Embodiment 2

[0074] Embodiment 2, vaccine composition

[0075] Circovirus antigen solution 50g (virus content before inactivation is 10 7.2 TCID 50 ), 50 g of the adjuvant composition prepared in Example 1 were first heated to 31° C., and then in a water bath, the stirring was started at a speed of 350 rpm, and the circovirus antigen solution was added to the adjuvant composition, stirred and emulsified The time is 10 minutes, and the stirring is completed. Put to 20 ℃. Particle size determination and stability tests were then carried out. The particle size was measured using a Malvern 3000 laser particle size analyzer, and IKAR W20 was used for stirring.

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Abstract

The invention relates to a w / o / w adjuvant composition. The w / o / w adjuvant composition comprises a sucrose fatty acid ester with HLB value of below 5, fatty alcohol-polyoxyethylene ether and oil. The vaccine composition prepared by the adjuvant composition is stable at different temperature and has no layering and demulsification phenomena, and the preparation condition of the vaccine is simple andmild. The prepared vaccine achieves the immune effect rapidly and does not have adverse effect during use.

Description

technical field [0001] The invention relates to a water-in-oil-in-water adjuvant composition, a vaccine composition prepared by using the adjuvant composition and a preparation method of the vaccine composition, belonging to the field of veterinary medicine. Background technique [0002] Bacterial, viral and parasitic infections are widely distributed in humans and animals. Diseases caused by these pathogens are often resistant to antimicrobial chemotherapy, leaving no effective treatment. Therefore, more and more people use vaccinology to control infectious diseases. Whole infectious pathogens can be rendered suitable for use in vaccine formulations after chemical inactivation or appropriate genetic engineering. Alternatively, protein subunits of pathogens can be expressed in recombinant expression systems and purified for use in vaccine formulation. Vaccines can be made more effective by including suitable adjuvants in the composition. [0003] An adjuvant generally re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61K39/23A61K39/135A61K39/245A61P31/14A61P31/20A61P31/22
CPCA61K39/39A61P37/04A61K39/12A61P31/20A61P31/22A61P31/14A61K2039/55511A61K2039/5258A61K2039/552C12N2750/10034C12N2710/16734C12N2750/14334C12N2770/32134
Inventor 田克恭刘永梅张许科
Owner LUOYANG SEIWEI BIOTECHNOLOGIES CO LTD
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