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Homogeneous immunodetection kit for detecting anti-cyclic citrullinated peptide antibody and application thereof

An anti-cyclic citrullinated peptide and immunodetection technology, applied in the field of immunoassay, can solve the problems of complicated operation, poor specificity, low sensitivity, etc., and achieve the effects of low technical requirements, simple operation and high sensitivity

Pending Publication Date: 2019-08-23
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The types of anti-citrulline antibodies in the human body include IgG, IgA, and IgM. The traditional indirect method can only detect IgG type antibodies
However, the indirect ELISA detection mode can not only detect IgG type antibodies, but also has low sensitivity, poor specificity, and cumbersome operation.

Method used

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  • Homogeneous immunodetection kit for detecting anti-cyclic citrullinated peptide antibody and application thereof
  • Homogeneous immunodetection kit for detecting anti-cyclic citrullinated peptide antibody and application thereof
  • Homogeneous immunodetection kit for detecting anti-cyclic citrullinated peptide antibody and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0064] As mentioned above, the current research or commercial anti-citrulline antibody kits are all detected by indirect method, especially by ELISA indirect method. The types of anti-citrulline antibodies in the human body include IgG, IgA, and IgM. The traditional indirect method can only detect IgG type antibodies. However, the indirect ELISA detection mode can not only detect IgG type antibodies, but also has low sensitivity, poor specificity, and cumbersome operation. For this reason, the inventors have conducted a lot of theoretical and experimental research on the anti-citrulline antibody detection kit.

[0065] The inventors found that the antigen obtained by the preparation method of the present invention can be specifically recognized and bound by the anti-citrulline antibody to be tested, forming a sandwich (solid-phase antigen-antibody-labeled antigen) immune complex can complete the determination of all types of antibodies. The present inventors have further studi...

specific Embodiment approach

[0096] According to some specific embodiments of the present invention, the method comprises:

[0097] Step R1, mixing the sample to be tested with the first composition and the second composition and reacting to obtain the first mixture;

[0098] Step R2, reacting after mixing the first mixture and the third composition to obtain the second mixture;

[0099] Step R3, contacting the energy or active compound with the second mixture to excite the donor to generate singlet oxygen, and the acceptor can react with the received singlet oxygen to generate a detectable chemiluminescent signal;

[0100] Step R4, detecting the presence and / or intensity of the chemiluminescent signal in step R3, so as to determine whether there is anti-cyclic citrullinated peptide antibody in the sample to be tested and / or determine the content of anti-cyclic citrullinated peptide antibody.

Embodiment approach

[0101] According to some embodiments of the present invention, the method further includes the step of preparing a standard working curve of anti-cyclic citrullinated peptide antibody before step R1.

[0102] In some specific embodiments of the present invention, in step R4, the intensity of the chemiluminescent signal in step R3 is detected, and the anti-cyclic citrullinated peptide antibody in the test sample is determined based on the standard working curve of the anti-cyclic citrullinated peptide antibody. Amino acid peptide antibody content.

[0103] In the present invention, there is no special limitation on the way or order of mixing the sample to be tested with the first composition and the second composition in step R1. For example, in some embodiments of the present invention, in step R1, the sample to be tested can be The sample is mixed with the first composition and the second composition and then reacted to obtain the first mixture; and in other embodiments of th...

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Abstract

The invention provides a homogeneous immunodetection kit for detecting an anti-cyclic citrullinated peptide antibody. The kit is based on a double antigen sandwich reaction mode and prepared by adopting high sensitivity and specificity of cyclic citrullinated peptide-coupled carrier protein as an antigen raw material; and the kit can detect all types of anti-citrulline antibodies and has the advantages of being strong in specificity, good in signal amplification effect, high in sensitivity, wide in linearity range, simple in operation, good in stability and high in precision.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, and in particular relates to a homogeneous immunoassay kit for detecting anti-cyclic citrullinated peptide antibody and its preparation method and use method. Background technique [0002] Anticyclic citrullinated peptide antibodies (Anticyclic citrullinated peptide antibodies, anti-CCP) antibody is a new type of serum marker. Clinical practice shows that the titer of anti-CCP antibody is usually related to the activity of the disease. Anti-CCP antibodies were detected in serum and synovial fluid very early, and this antibody has high specificity and certain prognostic value. [0003] The current research or commercial anti-citrulline antibody kits are all detected by indirect method, especially by ELISA indirect method. The types of anti-citrulline antibodies in the human body include IgG, IgA, and IgM. The traditional indirect method can only detect IgG type antibodies. However, the i...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/6854G01N33/54346G01N2333/47G01N2800/102
Inventor 饶星廖智星刘宇卉李临其他发明人请求不公开姓名
Owner CHEMCLIN DIAGNOSTICS CO LTD
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