Preparation method for modified polyacrylamide immobilization cell

Abstract

The present invention relates to a modified polyacrylamide immobilized cell preparation method, comprises the following steps: 1) modified polyacrylamide synthesis: using ammonium persulfate as initiator, formate solium as chain transferring agent for synthesizing low molecular weight polyacrylamide, hydroxymethylating polyacrylamide using formaldehyde, condensating it with acrylamide to obtain modified polyacrylamide 2) immobilized microbe cell preparation: mixing modified polyacrylamide aqueous solution and prepared bacteria suspension liquid, adding crosslinking agent and initiator, then adding catalyst tetramethylethylenediamine and mixing evenly, keeping still for several minutes to obtain immobilized cell gelatin.The coagulation speed of gelatin is quick under room temperature. Low coagulation temperature can keep embedment microbe activity. The prepared immobilized microbe cell gelatin can be used in alive cell and enzyme immobilization.

Description

technical field [0001] The invention relates to a preparation method of immobilized cells, in particular to a preparation method of immobilized microbial cells using modified polyacrylamide as a carrier. Background technique [0002] Among the methods of immobilization of microbial cells, the embedding method is the most commonly used. As embedding carriers, sodium alginate and carrageenan, which are natural polymer polysaccharides, are the most widely used. They have the advantages of curing, easy molding, low toxicity to microorganisms, and high immobilization density. However, they have poor resistance to microbial decomposition and low mechanical strength. Although the gels of these two carriers can be stabilized with cross-linking agents, the activity and mass transfer performance will decrease again. Carrier substances commonly used in the synthesis of polymer compounds include polyacrylamide, polyvinyl alcohol, and light-hardening resins. Their outstanding advantag...

AI Technical Summary

Problems solved by technology

However, the formation conditions of the polymer network are relatively severe, and the damage to microbial cells is relatively large.

In particular, polyacrylamide is used as a microbial cell embedding and immobilization carrier. Although its mechanical strength, chemical stability, anti-microbial decomposition performance and low price are superior to other synthetic polymer carriers, but because of the cytotoxicity of monomeric acrylamide , environmental toxicity, long polymerization reaction time, high polymerization temperature and other problems have seriously affected the use of immobilized microbial cells or immobilized enzymes in the fields of food, pharmaceuticals, environmental protection, new energy development and chemical synthesis.