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Application of complanatoside in preparation of medicine for treating skin inflammation

A technology of skin inflammation and sagarin, applied in the field of biomedicine, can solve the problems of lack of research on active ingredients, small toxic and side effects, and low dosage

Pending Publication Date: 2021-09-10
WUYI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many studies have shown that natural medicines have a certain relieving effect, there is a lack of research on their specific active ingredients, so it is urgent to find a drug that can effectively relieve skin inflammatory damage caused by various inflammatory cytokines, with less toxic side effects and low dosage. protective agent

Method used

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  • Application of complanatoside in preparation of medicine for treating skin inflammation
  • Application of complanatoside in preparation of medicine for treating skin inflammation
  • Application of complanatoside in preparation of medicine for treating skin inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 uses MTT method to measure the impact of different concentrations of sagarin on the activity of HaCaT cells

[0033] 1. Experimental method

[0034] Digest HaCaT cells from the culture flask with trypsin, centrifuge for 3 min, add DMEM medium containing 10% FBS and 1% antibiotics to make a cell suspension, and inoculate at a concentration of 5×10 4 Inoculated in 96-well plates (100 μL / well) at the concentration of cells / mL, placed in 5% CO 2 , and cultured in a 37°C incubator for 24 hours. Set the concentration of sagarin as 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 60 μM, and 80 μM, and set 5 replicate wells for each concentration. 5%CO 2 After culturing at 37°C for 24 hours, carefully aspirate the culture solution, and add 150 μL of MTT solution (0.5 mg / mL) prepared in serum-free medium to each well; after continuing to cultivate for 4 hours, carefully aspirate the culture solution in the well. Add 150 μL DMSO to each well and let it stand in the incubator for...

Embodiment 2

[0037] Example 2 MTT method was used to determine the effect of different concentrations of sagiloside on the activity of HaCaT cells treated with various inflammatory cytokine compositions for 24h, 48h and 72h respectively

[0038] 1. Experimental method

[0039] Digest HaCaT cells from the culture flask with trypsin, centrifuge for 3 min, add DMEM medium containing 10% FBS and 1% antibiotics to make a cell suspension, and inoculate at a concentration of 5×10 4Inoculated in 96-well plates (100 μL / well) at the concentration of cells / mL, placed in 5% CO 2 , and cultured in a 37°C incubator for 24 hours. Aspirate the culture solution, add 100 μL of serum-free medium to each well and culture for 24 hours, aspirate the culture solution, and combine interleukin-6 (20 ng / mL), tumor necrosis factor-α (40 ng / mL) and interferon-γ ( 80ng / mL) combination to stimulate HaCaT cells, set the concentration of sagarin to be 1 μM, 5 μM, 10 μM, and 20 μM, and set 5 duplicate wells for each con...

Embodiment 3

[0044] Example 3 Effect of treatment with different concentrations of sagarin on the level of IL-1β in cells treated with inflammatory cytokine composition

[0045] 1. Experimental method

[0046] HaCaT keratinocytes (1×10 6 cells / well) were seeded in 6-well plates in 5% CO 2 , and cultured in a 37°C incubator for 24 hours to allow the cells to attach to the well plate. Stimulate HaCaT cells with a combination of inflammatory cytokines interleukin-6 (20ng / mL), tumor necrosis factor-α (40ng / mL) and interferon-γ (80ng / mL), and set the concentration of sagarin to 1 μM and 5 μM , 10 μM, 20 μM, and treat the cells for 24 hours. According to the instructions of the ELISA kit, the level of IL-1β in the culture supernatant was determined by using it.

[0047] 2. Experimental results

[0048] Such as Figure 5 As shown, 1.0uM, 5.0uM, 10.0uM and 20.0uM sagarin decreased the level of IL-1β, respectively with statistical significance p<0.05, p<0.01, p<0.01 and p<0.001.

[0049] Suc...

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Abstract

The invention relates to the technical field of biological medicines, in particular to application of complanatoside in preparation of a medicine for treating skin inflammation. The research finds that various inflammatory cytokines can induce skin cell inflammatory response, so that the levels of IL-1beta, ROS and CCL22 are obviously increased; and the levels of IL-1beta, ROS and CCL22 can be reduced by treating HaCaT cells with the complanatoside, the inflammatory reaction induced by the combination of inflammatory cytokines can be inhibited, and a new way is provided for research and development of new drugs for resisting skin inflammation induced by various inflammatory cytokines.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of sagirin in the preparation of medicines for treating skin inflammation. Background technique [0002] The skin covers the surface of the body and is the first important barrier to protect the body from external damage. Skin damage can take many forms. The term "cytokine storm" was first coined in 1993 to describe graft-versus-host disease. A cytokine storm occurs when the immune system is overactivated by infection, drugs, or other stimuli, leading to elevated levels of cytokines (interferons, interleukins, chemokines, cerebrospinal fluid, tumor necrosis factor, etc.). Cytokines are released into the circulation with widespread and deleterious effects on multiple organs. It is well known that inflammatory cytokines are involved in the pathogenesis of inflammatory skin lesions. [0003] There have been many reports of skin lesions in COVID-19 patients aro...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K36/481A61P17/00A61P29/00
CPCA61K31/7048A61K36/481A61P17/00A61P29/00
Inventor 王潇郑希李冬利徐学涛张焜盛钊君吴盼盼刘文锋任想吴萌硕
Owner WUYI UNIV
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