Application of complanatoside in preparation of medicine for treating skin inflammation
A technology of skin inflammation and sagarin, applied in the field of biomedicine, can solve the problems of lack of research on active ingredients, small toxic and side effects, and low dosage
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0032] Example 1 Effect of different concentrations of Shayuan glycosides on HACAT cell activity was determined by MTT method
[0033] 1, experimental method
[0034] HACAT cells were digested from the culture flask with trypsin, centrifuged for 3 min, adding a cell suspension in DMEM medium containing 10% FBS and 1% antibiotic to inoculate concentration 5 × 10 4 The concentration of / ml is inoculated on a 96-well plate (100 μL / hole), placed in 5% CO 2 In the incubator at 37 ° C for 24 hours. The concentration of Shayuanzide was 1 μm, 5 μm, 10 μm, 20 μm, 40 μm, 60 μm, 80 μm, and 5 additional holes were provided for each concentration. 5% co 2 After 24 hours of culture at 37 ° C, the culture solution was taken out, and 150 μl of serum-free medium was added to the MTT solution (0.5 mg / ml) of the MTT (0.5 ml) of serum-free medium; after further culturing 4 h, it was carefully sued into the pore culture medium. 150 μl of DMSO was added to each well, and it was allowed to stand fo...
Example Embodiment
[0037] Example 2 Effect of HACAT Cell Activity of 24H, 48H and 72H for 24 h, 48h and 72h were determined by MTT method
[0038] 1, experimental method
[0039] HACAT cells were digested from the culture flask with trypsin, centrifuged for 3 min, adding a cell suspension in DMEM medium containing 10% FBS and 1% antibiotic to inoculate concentration 5 × 10 4The concentration of / ml is inoculated on a 96-well plate (100 μL / hole), placed in 5% CO 2 In the incubator at 37 ° C for 24 hours. Aspirated broth added to each well 100μL of serum-free medium for 24h, the culture medium was aspirated, in combination with inflammatory cytokines interleukin -6 (20ng / mL), tumor necrosis factor -α (40ng / mL) and interferon [gamma] ( 80ng / mL) combined HaCaT cells stimulated provided complanatus sub glycoside concentration 1μM, 5μM, 10μM, 20μM, the concentration of each of 5 wells is provided. 5% co 2 , 37 [deg.] C culture 24h, 48h, and 72h after carefully aspirated out of the culture medium,...
Example Embodiment
[0044] Example 3 Different concentrations embodiment complanatus sub-glucoside treated cells Effect of the level of IL-1β treatment of inflammatory cytokines composition
[0045] 1, experimental method
[0046] The HaCaT keratinocytes (1 × 10 6 Cells / well) were seeded in 6 well plates, placed in 5% CO 2 , In 37 ℃ incubator for 24 hours, the cells adhered to the plate. With inflammatory cytokines interleukin -6 (20ng / mL), a combination of tumor necrosis factor -α (40ng / mL) and interferon -γ (80ng / mL) stimulated HaCaT cells, provided the concentration of glycosides complanatus sub 1μM, 5μM , 10μM, 20μM, cells were treated for 24 hours. Follow the instructions of ELISA kits, with which the level of the culture supernatant was measured IL-1β is.
[0047] 2, experimental results
[0048] like Figure 5 Shown, 1.0uM, 5.0uM, 10.0uM 20.0uM and the sub complanatus glycosides reduces IL-1β levels of, respectively, with statistical significance p <0.05, p <0.01, p <0.01 and p <0.001. ...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap