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Method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot

A technique for proanthocyanidin and Aronia nigricans, which is applied in the field of rapid extraction of proanthocyanidins, can solve problems such as difficulty in widespread promotion of an enzyme extraction method, and achieve the effects of fast and convenient method, loss avoidance, and fast and complete extraction.

Active Publication Date: 2017-05-17
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic extraction of proanthocyanidins is environmentally friendly and pollution-free, but the technology is still in the process of exploration, and there are certain difficulties in the widespread promotion of enzymatic extraction.

Method used

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  • Method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot
  • Method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot
  • Method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Investigation of the Factors Affecting the Extraction of Proanthocyanidins from Sorbus arvensis Pomace

[0024] Using the Aronia adenocarpa pomace as raw material, the effects of different extraction conditions on the extraction of proanthocyanidins from Aronia arvensis pomace were investigated by single factor experiments. The specific extraction conditions were: 1) the concentration of edible ethanol in the extract; 2 ) extraction time; 3) extraction voltage; 4) extraction solid-liquid ratio; 5) extraction times.

[0025] The test process is as follows: Weigh 5.00g of Sorbus arvensis pomace and place it in a plastic beaker, use a flash extraction device to extract according to the conditions shown in Table 1-Table 5, and the extract is centrifuged at 5000×g for 10 minutes in a centrifuge , combine the supernatants, set the volume to 250mL, accurately measure 1mL, dilute five times, and then accurately measure 1mL into a 10mL plastic centrifuge tube, add chro...

Embodiment 2

[0043] Example 2 Comparison of the extraction effects of proanthocyanidins in Aronia slag by different extraction methods

[0044] Using 5.00g of Aronia pomace as raw material, the differences in the effects of ultrasonic extraction, microwave extraction and flash extraction in extracting proanthocyanidins from Aronia pomace were investigated.

[0045] During the investigation of the three extraction methods, except that the extraction process is different, other processing and content determination processes are the same as the processing and determination methods in Example 1. The extraction conditions of each method are shown in Table 6:

[0046] Table 6 Comparison of three extraction methods

[0047] group method of extraction extract solid to liquid ratio time Voltage power temperature content 1 flash extraction 50% ethanol 1:40 10min 60V - 30℃ 11.679％ 2 ultrasonic extraction 50% ethanol 1:40 30min - 500W(30KHz) 30℃ ...

Embodiment 3

[0050] 1) Soak 5.00 g of Aronia fruit in 200 mL of ethanol solution with a concentration of 50% (V / V). After the completion, the extract is filtered with suction, and the residue is removed by filtration to obtain a solution of Aronia nigra proanthocyanidins extract;

[0051] 2) Use a negative pressure rotary evaporator to concentrate the solution of the proanthocyanidin extract of Aronia nigricarpa (at a temperature of 30° C.) to obtain the extract of the proanthocyanidin extract of Aronia nigricarpa;

[0052] 3) Pre-freeze the extract of Aronia proanthocyanidins in a -80°C ultra-low temperature refrigerator, and then freeze-dry it with a freeze-drying device to obtain a freeze-dried powder of Aronia proanthocyanidins extract.

[0053] The obtained lyophilized powder of the proanthocyanidin extract of Aronia nigricarpa, weighed 154.6 mg, weighed a certain amount and reconstituted it, and measured the proanthocyanidin content of 89.71 mg by ultraviolet spectrophotometer, that ...

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Abstract

The invention relates to a method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot. The method comprises the steps of dipping the fruit or pomace of the aronia melanocarpa elliot into an organic solvent, conducting Flash extraction for 0.5-30 minutes by using a Flash extractor, carrying out suction filtration and centrifugation to obtain a procyanidine extract solution of the aronia melanocarpa elliot; concentrating the procyanidine extract solution of the aronia melanocarpa elliot by using a negative pressure rotary evaporation device to obtain procyanidine extract concrete of the aronia melanocarpa elliot; and after pre-freezing the procyanidine extract concrete of the aronia melanocarpa elliot by using an ultra-low temperature refrigerator, carrying out freeze drying on the procyanidine extract concrete by using a freeze drying device to obtain the freeze-dried powder of a procyanidine extract of the aronia melanocarpa elliot. According to the method disclosed by the invention, low temperature in the whole course can be realized, procyanidine disintegration caused by high temperature can be avoided, the loss of anthocyanin in the extracting process effectively can be effectively avoided, the operation is simple and the cost is low, and promotion in enterprises can be achieved by forming an automatic production line, so that the method has a relatively high commercial value.

Description

technical field [0001] The invention belongs to the technical field of extraction of effective natural components in plants, and specifically relates to a method for rapidly extracting proanthocyanidins from Sorbus arvensis fruit or pomace by using flash extraction technology at low temperature throughout the whole process. Background technique [0002] Proanthocyanidins (Oligomeric Proanthocyanidins, OPC) is a polyphenolic compound widely present in the core, skin or seeds of plants. It has a strong antioxidant effect and is widely used in the fields of food, medicine and cosmetics. It has various functions such as anti-cancer, anti-cancer, prevention and treatment of cardiovascular diseases, anti-hyperglycemia, anti-radiation, immune regulation, improvement of human microcirculation and skin protection. It is one of the most popular natural active ingredients of plants in European and American countries. [0003] Aronia prunifolia Viking. and Aronia melanocarpa, Rosaceae, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 陈立江崔庆国梁啸赵京华刘宇
Owner LIAONING UNIVERSITY
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