Method for producing high fructose syrup by immobilized glucose isomerase

A technology of glucose isomerase and high fructose syrup, applied in isomerase, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problems of difficult industrial application, cumbersome, low yield and the like , to achieve the effect of high reuse efficiency, high enzyme load and simple operation

Inactive Publication Date: 2016-10-26
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional high fructose syrup production mainly uses acid hydrolysis starch, but this traditional acid hydrolysis process has many disadvantages: it needs acid-resistant and pressure-resistant equipment; it needs refined starch as raw material; the yield of starch converted into glucose is low, not more than 90%; During the hydrolysis process, more oligosaccharides with bitter taste are produced due to the reverse polymerization reaction of glucose, which must be refined by crystallization to remove the bitter taste, etc.
However, the raw material utilization rate of the former is low and the cost is large, so it is difficult to be applied to industry; the inulinase of the latter has no commercial products, the production cost is high, and browning is prone to occur during hydrolysis; and the free enzyme intermittent production method has the advantages of Many disadvantages: the extraction and purification of enzymes must use very delicate and even cumbersome methods, which are expensive; after the enzymes are separated from the cells, their stability is significantly reduced; these water-soluble enzymes must be reacted in batches. Extremely difficult to recycle, resulting in waste and high product cost; intermittent operation, resulting in poor product quality, etc.
However, these immobilization methods have high cost and low conversion rate of HFCS, and are difficult to scale up in industrial production.

Method used

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  • Method for producing high fructose syrup by immobilized glucose isomerase
  • Method for producing high fructose syrup by immobilized glucose isomerase

Examples

Experimental program
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Effect test

example 1

[0022] Dissolve 200 g of glucose isomerase in 1000 mL of phosphate buffer (pH=7.4), the amount of glucose isomerase in the system is 0.2 g / mL, and the glucose isomerase solution is filtered by pumping (filtering The method is filtration, gravity filtration, pressure filtration, vacuum filtration, centrifugal filtration or cross-flow filtration) through a polyvinylidene fluoride flat membrane with an asymmetric structure, and the filtration direction is from the loose side of the membrane to the dense side, and the glucose isomerization The enzyme is almost completely immobilized in the loose layer of the membrane to obtain an immobilized enzyme membrane reactor; 40% glucose solution is pumped into the immobilized enzyme membrane reactor, and the feed liquid flows back into the enzyme membrane reactor for a cyclic reaction. The reaction temperature is 50 ℃, the reaction time was 4 h; after the reaction, the fresh glucose solution was replaced, and the above reaction was restarte...

example 2

[0024] Dissolve 200 g of glucose isomerase in 1000 mL of phosphate buffer (pH=7.4), the amount of glucose isomerase in the system is 0.2 g / mL, and the glucose isomerase solution is filtered by pumping (filtering The method is filtration, gravity filtration, pressure filtration, vacuum filtration, centrifugal filtration or cross-flow filtration) through the polyacrylonitrile hollow fiber membrane with an asymmetric structure, and the filtration direction is from the loose side of the membrane to the dense side, and the glucose isomerization The enzyme is almost completely immobilized in the loose layer of the membrane to obtain an immobilized enzyme membrane reactor; then the membrane is put into the membrane reactor to make an immobilized enzyme membrane reactor; 40% glucose solution is pumped into the immobilized enzyme membrane to react The feed liquid flowed back into the enzyme membrane reactor for a circular reaction, the reaction temperature was 60°C, and the reaction tim...

example 3

[0026] Dissolve 500 g of glucose isomerase in 1000 mL of phosphate buffer (pH=7.4), the amount of glucose isomerase in the system is 0.5 g / mL, and the glucose isomerase solution is filtered by pumping (filtering The method is filtration, gravity filtration, pressure filtration, vacuum filtration, centrifugal filtration or cross-flow filtration) through a polysulfone flat membrane with an asymmetric structure, the filtration direction is from the loose side of the membrane to the dense side, and the membrane converts glucose Almost completely immobilized in the loose layer of the membrane to obtain an immobilized enzyme membrane reactor; then put this membrane into the membrane reactor to make an immobilized enzyme membrane reactor; pump 50% glucose solution into the immobilized enzyme membrane reactor , the feed liquid flowed back into the enzyme membrane reactor for cyclic reaction. The reaction temperature was 60 °C and the reaction time was 5.5 h. reached 97.9%, and the imm...

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Abstract

The invention discloses a method for producing high fructose syrup by immobilized glucose isomerase. The method comprises the following steps: immobilizing glucose isomerase in an ultra-filtration membrane with an asymmetric structure in a filtering mode to prepare a membrane bioreactor of the immobilized glucose isomerase; performing reaction on the glucose solution in batches or continuously by utilizing the membrane bioreactor to produce the high fructose syrup. According to the method, an enzyme catalysis process and a membrane separating process are integrated, so that the production process is simplified, and the activity and the stability of an enzyme are improved; meanwhile, an enzyme membrane reactor can be used repeatedly, solvent cleaning and batchwise feeding are avoided, the development direction of green chemistry is met, continuous production is realized, the production efficiency is improved, and reference is provided for industrial production of fructose.

Description

technical field [0001] The invention relates to a method for producing high fructose syrup by immobilizing glucose isomerase. Background technique [0002] High fructose syrup (HFCS), also known as isomerized syrup, mainly uses starch as raw material in industry. It is hydrolyzed into glucose through double-enzyme liquefaction and saccharification, and then a part of α-glucose isomerized by the isomerization of glucose isomerase. into α-fructose to obtain a mixture of α-glucose and α-fructose. Compared with traditional sucrose, high fructose syrup has higher sweetness and lower calories, and has health and nutritional benefits. As a natural sweetener, it is widely used not only in the food industry, but also in medicine, household condiments, and daily chemicals. Today, HFCS has become one of the important sweeteners, and its production development momentum is strong, with an annual world production of more than 10 million tons. [0003] Traditional high fructose syrup pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/24C12P19/02C12N11/14C12N11/12C12N11/08C12N11/04
CPCC12P19/24C12N9/92C12N11/04C12N11/08C12N11/12C12N11/14C12P19/02C12Y503/01018
Inventor 胡梦欣
Owner ZHEJIANG GONGSHANG UNIVERSITY
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