High-stability creatine kinase detection kit

Abstract

The invention relates to the field of in-vitro diagnosis biochemical reagents, particularly a high-stability creatine kinase detection kit. The high-stability creatine kinase detection kit is composed of a reagent R1 and a reagent R2 which are mutually independent, wherein the reagent R1 is composed of a biological buffer solution, an enzyme protective agent, a reaction substrate, a complex, a surfactant, a preservative and an activator; the reagent R2 is composed of a biological buffer solution, a reaction substrate and a preservative; and the volume ratio of the reagent R1 to the reagent R2 is 4:1. After adding the enzyme protective agent into the detection kit, the enzyme protective agent can be adopted to protect the enzyme on the premise of enhancing the stability of the reagent. After adding the enzyme protective agent, the enzyme can keep stable in the water solution for a long time, and the activity of the enzyme is not influenced.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic biochemical reagents, in particular to a creatine kinase detection kit with high stability. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) usually exists in the cytoplasm and mitochondria of animal heart, muscle and brain tissues. It is an important kinase that is directly related to intracellular energy operation, muscle contraction and ATP regeneration. It is reversible Catalyzes the transphosphorylation reaction between creatine and ATP. Creatine kinase has four isozyme forms: muscle (MM), brain (BB), hybrid (MB) and mitochondrial (MiMi). The MM type mainly exists in various muscle cells, the BB type mainly exists in brain cells, the MB type mainly exists in cardiomyocytes, and the MiMi type mainly exists in myocardial and skeletal muscle mitochondria. [0003] The clinical determination of creatine kinase refers to the total CK activity, which is mainly used for the di...

AI Technical Summary

Problems solved by technology

The electrophoresis method has the disadvantages of insufficient sensitivity, long operation time, and cumbersome operation. Although the chemiluminescence method has high sensitivity, its poor repeatability also hinders its large-scale application in clinical practice, while the enzymatic method has high enzyme activity and high detection sensitivity. advantages, but also has the disadvantage that the enzyme is easily inactivated in the buffer