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Multi-index protein chip inspection reagent unit of cardiovascular disease diagnosis and prediction

A cardiovascular and kit technology, applied in the field of in vitro clinical tests and biochips, can solve problems such as time-consuming and labor-intensive

Inactive Publication Date: 2006-11-01
穆海东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the diagnosis and prediction of cardiovascular diseases are mainly based on the detection of a single index, which is time-consuming and laborious

Method used

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  • Multi-index protein chip inspection reagent unit of cardiovascular disease diagnosis and prediction
  • Multi-index protein chip inspection reagent unit of cardiovascular disease diagnosis and prediction
  • Multi-index protein chip inspection reagent unit of cardiovascular disease diagnosis and prediction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of detection reaction plate

[0037]Take a 48-well plate, set 42 sample wells and 6 standard wells. Place a solid phase support (NC membrane or PDVF membrane) at the bottom of each reaction well, and the solid phase support is coated with anti-CRP, anti-Myoglobin, anti-cTnI, anti-cTnT, anti-NT-proBNP, anti-CK-MB , protein microarrays of eight antibodies including anti-FABP and anti-GPBB ( figure 1 ).

Embodiment 2

[0039] Kit preparation

[0040] Prepare a test kit containing eight indicators of cardiovascular disease integrated detection, which contains a reaction well plate (24-48 people) of embodiment 1, 1 bottle of washing concentrate, 1 bottle of enzyme-labeled working solution, and 1 bottle of detection solution A , a bottle of detection solution B, and a standard bottle.

[0041] Washing Concentrate, Enzyme Label Working Solution, Detection Solution A, Detection Solution B

Embodiment 3

[0043] Integrated detection of eight indicators of cardiovascular disease

[0044] Test each blood sample according to the following method:

[0045] 1. Add test blood or standard substance to the corresponding reaction well, 75ul / well.

[0046] 2. Add enzyme-labeled working solution, 75ul / well.

[0047] 3. Incubate and shake at 37°C for 60 minutes.

[0048] 4. Wash 4-6 times with lotion, 2 minutes each time, vibrate.

[0049] 5. Add detection solution 20ul / well, so that the detection solution is evenly distributed at the bottom of the well.

[0050] 6. CCD detection, exposure for 30-60 seconds depending on the strength of the luminescent signal.

[0051] Result judgment:

[0052] According to the CutOff value of different indicators, refer to the gradient curve of the standard to determine the negative or positive of the sample serum.

[0053] At the same time, the serum samples tested were routinely tested by conventional methods. The result shows that the test result...

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Abstract

A detection kit of multiindex protein for diagnosing cardiovascular disease is prepared as having base plate and reaction holes on it set on reaction hole plate; including 12-200 sample holes and 5-8 standard piece holes in said reaction holes as each reaction hole bottom being set with solid phase carrier; enveloping micro lattice of eight antibody as anti-CRP, anti-Myoglobin, anti-cTnI, anti-cTnT, anti-NT-proBNP, anti-CK-MB, anti-H-FABP and anti-GPBB on said carrier for accurately diagnose said disease in multiple man-share.

Description

technical field [0001] The invention belongs to the field of in vitro clinical testing and biochip technology, specifically, it is an eight-item index (CRP, Myoglobin, cTnI, cTnT, NT-proBNP, CK-MB) suitable for the diagnosis, prediction and prognosis of cardiovascular diseases. , FABP and GPBB) reaction plates and kits for integrated detection Background technique [0002] Cardiovascular disease includes diseases of the heart and vascular system. Since the 20th century, with the advancement of human civilization, medical technology has been greatly developed, and infectious diseases have been gradually controlled. The development of the economy and the changes in people's living standards and lifestyles have brought about significant changes in the spectrum of human diseases, that is, changes in the direction of chronic non-communicable diseases. Among them, cardiovascular disease has gradually become the main cause of death in Western countries. The death toll caused by c...

Claims

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Application Information

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IPC IPC(8): G01N33/543C09K11/00G01N33/531
Inventor 穆海东
Owner 穆海东
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