Cultivating and producing method for haematococcus pluvialis

Haematococcus pluvialis, production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bulk chemical production, etc., can solve energy waste, cooling and heating, very demanding cultivation conditions, pluvialis red Problems such as the difficulty in cultivating algae can achieve the effects of saving energy, avoiding difficult temperature control, and not being prone to biological pollution

Inactive Publication Date: 2012-11-07
陈勇
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0010] Therefore, the cultivation of Haematococcus pluvialis is relatively difficult, and the cultivation conditions such as temperature and light are very demanding
Most of the natural lighting methods used in current scientific research and production are difficult to achieve precise temperature control
Especially in summer and winter, a lot of energy is wasted in cooling in summer and heating in winter

Method used

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  • Cultivating and producing method for haematococcus pluvialis
  • Cultivating and producing method for haematococcus pluvialis

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Embodiment Construction

[0025] Method of the present invention is as follows:

[0026] 1. Cultivation environment

[0027] The culture container is a sealed tank with a volume of 10 cubic meters, and the material of the tank is glass reinforced plastic (FRP or GRP) or stainless steel, or enamel. The selection medium was GB11 medium. In the cultivation and production process, pipes or jackets are used to achieve heat preservation.

[0028] Reactor Sterilization: Reactors are sterilized by chemical or steam methods.

[0029] Sterilization of the medium: chemically sterilize the medium with hypochlorous acid or high temperature sterilization. After chemical sterilization, the medium is neutralized to pH 6.0-8.5, and the temperature of the medium is adjusted to below 28°C after sterilization.

[0030] 2. Inoculation and cultivation

[0031] Inoculate the medium with green motile cells of Haematococcus pluvialis. The inoculation density is 60,000 / ml. Artificial LED light source was used for cultiva...

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Abstract

The invention discloses a cultivating and producing method for haematococcus pluvialis. A haematococcus pluvialis green swarm cell is inoculated to a culture medium in a sealed tank body, a manual light-emitting diode (LED) light source is utilized for irradiation cultivating, the wavelength range of a monochromatic light emitted by the LED light source is 450nm to 640nm with the bandwidth of 30nm, two types of light sources with different wavelengths are simultaneously used according to the proportion of light intensity of red lights and blue lights, which is in a range from 2:1 to 5:1, the total light intensity is in a range from 80mu E / m2.s to 1500mu E / m2.s, the temperature during the cultivating process is maintained in a range from 15 DEG C to 28 DEG C, and the potential of hydrogen (pH) of the cultivating environment is controlled in a range from 8.0+ / -1.0 by adding carbon dioxide. The energy consumption of temperature controlling is remarkably saved, the cultivating period is shortened by above 50% compared with that of traditional methods, and the concentration of the obtained haematococcus pluvialis cell in a green algae liquid is 2 to 5 times of that of the haematococcus pluvialis cell in a green algae liquid by traditional methods.

Description

technical field [0001] The invention relates to a method for cultivating Haematococcus pluvialis on an industrial scale. The Haematococcus pluvialis belongs to freshwater algae. Background technique [0002] Haematococcus pluvialis, also known as Haematococcus pluvialis or Haematococcus lacustrine, belongs to Volvox, Haematococcus family. [0003] In 1899, it was discovered that this algae is often attached to water tanks or shallow water bays near the sea in the form of a blood-red shell. The life course of this algae goes through a red dormant stage, followed by A green swimming phase followed by a red dormant phase. [0004] In 1934, Elliot added details of the growth history of this alga from the point of view of cell morphology. Four typical cell shapes appear throughout the life cycle: small worm body, large worm body with flagella, gelatinous sheath body with no motility, and red large cells with hard cell walls-red cysts (Haematocysts). In a clean environment wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCY02P20/54
Inventor 陈勇邹宁
Owner 陈勇
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