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Method for recycling hemp strain-containing degumming liquid for degumming hemp

A hemp and degumming technology, applied in the direction of bacterial retting, etc., can solve the problems of low hemp production rate, long degumming cycle, large sewage discharge, etc., and achieve the effect of increasing the hemp production rate, improving the quality of hemp fiber, and saving energy consumption

Inactive Publication Date: 2011-09-07
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the problems of long degumming period, low hemp yield, low strength of hemp fiber and large amount of sewage discharge during retting in the existing hemp degumming method, and provides a method for degumming hemp by reusing hemp plus bacteria degumming liquid method

Method used

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Experimental program
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specific Embodiment approach 1

[0015] Specific embodiment one: the method that this embodiment marijuana adds bacterium degumming liquid and reuses and carries out hemp degumming realizes by following steps: One, the activation of bacterial classification for hemp degumming: Escherichia coli HDDMG05 and bacillus licheniformis HDDMM02 are respectively in 30 Cultivate on beef extract peptone medium for 24 hours at ℃; 2. First-level seed culture: use an inoculation loop to pick the activated bacteria in step 1 and insert them into 200-500mL industrial expansion medium, at 28-32℃ Cultivate for 20 to 30 hours; 3. Secondary seed cultivation: Inoculate the inoculum of 0.5% to 2% of the inoculum after the primary seed cultivation into 200 to 500L industrial expansion medium, at 28 to 32°C Cultivate under conditions for 20-30 hours; Fourth and third-level seed cultivation: insert the inoculum of 0.5% to 2% of the inoculum of the second-level seed culture into 2-5t industrial expansion medium according to the volume f...

specific Embodiment approach 2

[0021] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 2, use the inoculation loop to pick the activated strains of step 1 and insert them into 250-400mL industrial expansion medium, and cultivate them at 30°C for 24h . Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0022] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step three, the inoculation amount of 1% to 1.5% of the inoculum after the primary seed culture is inserted into 250 ~ 400L industrial expansion medium cultured at 30°C for 24 hours. Other steps and parameters are the same as those in the first embodiment.

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Abstract

The invention relates to a method for recycling hemp strain-containing degumming liquid for degumming hemp, relating to a hemp degumming process method and mainly solving the problems of the traditional hemp degumming method, such as long degumming period, low fiber yield, low hemp fiber strength and large sewage discharge during retting. The method disclosed by the invention comprises the following steps of: activating strains used for degumming hemp; carrying out primary seed culturing; carrying out second-degree seed culturing; carrying out third-degree seed culturing; degumming hemp with strains; recycling hemp strain-containing degumming liquid and adding to a new hemp degumming pond, and then adding hemp stalks, sodium chloride and urea, filling clean water into the hemp degumming pond, increasing the temperature for retting and completing the degumming process when a retting terminal point is reached. By adopting the method disclosed by the invention, 50% of waste water discharge can be reduced every day, energy consumed for degumming hemp is saved by 10%-15%; meanwhile, the degumming period of the hemp stalks is shortened to 80-120h, the hemp fiber yield is improved by 3%-5%, the hemp fiber strength is enhanced to 220-280N, and the quality of hemp fiber is improved.

Description

technical field [0001] The present invention relates to a method of degumming cannabis. Background technique [0002] At present, the hemp degumming method adopted in our country is: firstly bundle the hemp stalks into small bundles with a diameter of 20 cm, then put the bundled hemp stalks into the degumming pool with a loading density of 75-80 kg / m3, and then place the hemp stalks in the degumming pool Fill it up, heat the retting pool to the required temperature with steam after filling, and use steam to replenish the temperature to the required temperature once a day during the degumming process of cannabis. When judging the end point of retting, the sensory method is adopted. The sensory method mainly refers to the "stem-pulling method": breaking the retting stem at the position of one-third of the root of the stem, so that the xylem can be separated from the fiber The part is drawn out to reach the end of retting. [0003] The fermentation cycle of hemp degumming in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01C1/04C12S11/00
Inventor 蔡柏岩凌宏志葛菁萍平文祥接伟光王贵宾江云飞
Owner HEILONGJIANG UNIV
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