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Bacillus cereus DA3 strain and preparation method and application thereof

A technology of Bacillus cereus and strains, applied in the field of Bacillus cereus DA3 strains and its acquisition, can solve the problems of not meeting the requirements of spinning, not being able to obtain satisfactory results, and not being practically applied, etc. Pollution, suitable for large-scale industrial production, and short degumming time

Inactive Publication Date: 2011-04-06
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The other two bacteria also degrade the fiber cell wall during degumming, but due to a series of requirements for the strains in industrial production, they have not been practically used at present.
So far, although a lot of research has been done on the degumming of flax by biological methods at home and abroad, the pure enzymatic degumming can not get satisfactory results, and it can not meet the requirements of spinning, and all of them are laboratory experiments. Research

Method used

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  • Bacillus cereus DA3 strain and preparation method and application thereof
  • Bacillus cereus DA3 strain and preparation method and application thereof
  • Bacillus cereus DA3 strain and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Screening and Obtaining of Flax Degumming Strains

[0059] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 37°C for 1 to 4 days, obtained from the wild-type degumming dominant strain;

[0060] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of sterile water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 9 with NaOH;

[0061] (2) Inoculate 4 loops of the bacterial strain obtained in step (1) into pectin nutrient medium (petri dish diameter 10cm), cultivate at 28°C for 72h, after the formation of col...

Embodiment 2

[0080] In order to enable the strain to maintain a stable high-yielding characteristic, beef extract peptone medium is used as the slant preservation medium. The raw materials and preparation methods of the medium are: 0.5g beef extract, 1g peptone, and 0.5g sodium chloride (pH7.4-7.6) , add water to 100ml constant volume, culture overnight at 37°C 200rpm, add 100ml of freezing buffer; among them, the freezing buffer consists of 0.0627g potassium dihydrogen phosphate, 0.0177g dipotassium hydrogen phosphate, 0.0588g sodium citrate, sulfuric acid heptahydrate Magnesium 0.02645g, glycerin 10ml, add water to volume to 100ml; divide nutrient broth medium (beef extract 0.3g, peptone 1g, sodium chloride 0.5g, add water to 100ml volume, pH7.4-7.6) In 250mL containers, 50mL in each container, after sealing and bandaging, sterilize at 121°C for 20min; after cooling, insert the bacteria loop cultured on a slant for 24h, and shake the flask at a speed of 200r / min and a temperature of 40°C ...

Embodiment 3

[0090] The flax degumming process is as follows:

[0091] Put 5g of the original flax stem into a 250ml conical flask, divide the flax flax and the fermented bacteria liquid into the conical flask at a bath ratio of 1:20, the liquid volume is 100ml, degumming in a shaker at 40°C and 200rpm for 1 day , 2 days and 4 days; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5 minutes, rinse with tap water several times to remove bacteria on the linen, and stop degumming.

[0092] Through the observation of degummed flax, it was found that the flax fibers were obviously dispersed after 1 day, and the color of the fermentation broth became dark and turbid; after 2 days of degumming, the original bundled flax was completely dispersed into fibers, and the fermentation broth was obviously thickened; after 4 days of degumming, the degumming rate of flax reached More than 90.

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Abstract

The invention relates to a bacillus cereus DA3 strain and a preparation method and application thereof. The strain has the capacity of producing pectinase and hemicellulase and has flax retting activity. The provided strain and a culture system can be directly used for flax retting and have the advantages that: the retting period is short, the fiber dispersion rate is 100 percent, the retting efficiency is 95 percent, and the strain is not easily polluted, and is low in treatment cost, high in fiber quality, mild in retting condition, high in pollution resistance, good in heat resistance, free of environmental pollution and the like. Compared with the prior art, the invention has the characteristics of simple technology, suitability for large-scale industrial production and the like, and has potential theoretical and practical significance for exploring flax retting of the bacillus cereus under the alkaline condition.

Description

technical field [0001] The invention belongs to the field of bacillus cereus strains and applications thereof, in particular to a bacillus cereus DA3 strain and its obtaining method and application. Background technique [0002] Flax is an ancient bast fiber crop and oil crop. Flax originated in the Near East and along the Mediterranean coast. As early as the Neolithic Age more than 5,000 years ago, Swiss lake dwellers and ancient Egyptians had cultivated flax and used its fibers to weave clothing. "Mummy" in various parts of Egypt were also covered with linen. Flax for oil is also called flax. Flax has been cultivated in my country for at least 1,000 years. Fiber type flax was introduced from Japan in 1906. China is mainly distributed in Heilongjiang and Jilin provinces. Linen likes cool, humid climates. Flax fiber has the characteristics of strong tensile force, softness, good fineness, weak conductivity, fast water absorption and dispersion, and high expansion rate....

Claims

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Application Information

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IPC IPC(8): C12N1/20D01C1/04D06M16/00C12S11/00C12R1/085D06M101/06
Inventor 丁若垚董政娥郁崇文张兴群
Owner DONGHUA UNIV
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