Bacillus cereus DA3 strain and preparation method and application thereof

A technology of Bacillus cereus and strains, applied in the field of Bacillus cereus DA3 strains and its acquisition, can solve the problems of not meeting the requirements of spinning, not being able to obtain satisfactory results, and not being practically applied, etc. Pollution, suitable for large-scale industrial production, and short degumming time

Inactive Publication Date: 2011-04-06
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The other two bacteria also degrade the fiber cell wall during degumming, but due to a series of requirements for the strains in industrial production, they have not been practically used at present.
So far, although a lot of research has be

Method used

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  • Bacillus cereus DA3 strain and preparation method and application thereof
  • Bacillus cereus DA3 strain and preparation method and application thereof
  • Bacillus cereus DA3 strain and preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0057] Example 1

[0058] Screening of flax degumming strains

[0059] (1) Sampling rotten seaweed from the sea area of ​​Zhoushan, East China Sea, firstly, the seaweed and the enriched medium were cultured at room temperature for 30 days in the enriched medium at a ratio of 1 g: 20 ml, and then 0.1 ml of the enriched medium was taken and spread on the separation medium. culture medium at 37°C for 1 to 4 days, obtained from the wild-type degumming dominant strain;

[0060] Wherein, the enrichment medium formula is: flax meal 5g, sterile water 200ml, pH is natural; separation medium formula is: flax meal 5g, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, agar 20g, water 1000mL, pH adjusted to 9 with NaOH;

[0061] (2) Inoculate 4 rings of the strain obtained in step (1) into pectin nutrient medium (Petri dish diameter of 10cm), cultivate at 28°C for 72h, after colony formation, add 0.2% Congo red aqueous solution for staining for 4h, pour Remo...

Example Embodiment

[0079] Example 2

[0080] In order to maintain the stable high-yield characteristics of this strain, beef extract peptone medium is used as the slant preservation medium. The raw materials and preparation methods of the medium are: beef extract 0.5g, peptone 1g, sodium chloride 0.5g (pH 7.4-7.6) , add water to a constant volume of 100ml, incubate at 37°C at 200rpm overnight, and add 100ml of freezing buffer; wherein, the freezing buffer consists of potassium dihydrogen phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, sodium citrate 0.0588g, heptahydrate sulfuric acid Magnesium 0.02645g, glycerol 10ml, add water to volume to 100ml to prepare; divide the nutrient broth medium (beef extract 0.3g, peptone 1g, sodium chloride 0.5g, add water to 100ml to volume, pH7.4-7.6) In a 250mL container, 50mL in each container, after sealing and bandaging, sterilize at 121°C for 20min; after cooling, insert a bacterial ring that has been cultured on a slope for 24h, and culture in a...

Example Embodiment

[0089] Example 3

[0090] The flax degumming process is as follows:

[0091] 5g of flax stems were placed in a 250ml conical flask, and the flax stems and the fermented bacteria liquid were divided into the conical flasks at a bath ratio of 1:20, and the liquid filling amount was 100ml, and were degummed for 1 day at 40° C., 200rpm shaker respectively. , 2 days and 4 days; when the degumming time is up, remove the degumming liquid, boil it in a boiling water bath for 5 minutes, rinse with tap water several times to remove bacteria on the flax, and stop degumming.

[0092] Through the observation of degummed flax, it was found that the flax fibers were obviously dispersed after 1 day, and the color of the fermentation broth became darker and turbid; after 2 days of degumming, the flax that was originally bundled was completely dispersed into fibers, and the fermentation broth became thicker obviously; after 4 days of degumming, the degumming rate of flax reached more than 90 p...

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Abstract

The invention relates to a bacillus cereus DA3 strain and a preparation method and application thereof. The strain has the capacity of producing pectinase and hemicellulase and has flax retting activity. The provided strain and a culture system can be directly used for flax retting and have the advantages that: the retting period is short, the fiber dispersion rate is 100 percent, the retting efficiency is 95 percent, and the strain is not easily polluted, and is low in treatment cost, high in fiber quality, mild in retting condition, high in pollution resistance, good in heat resistance, free of environmental pollution and the like. Compared with the prior art, the invention has the characteristics of simple technology, suitability for large-scale industrial production and the like, and has potential theoretical and practical significance for exploring flax retting of the bacillus cereus under the alkaline condition.

Description

technical field [0001] The invention belongs to the field of bacillus cereus strains and applications thereof, in particular to a bacillus cereus DA3 strain and its obtaining method and application. Background technique [0002] Flax is an ancient bast fiber crop and oil crop. Flax originated in the Near East and along the Mediterranean coast. As early as the Neolithic Age more than 5,000 years ago, Swiss lake dwellers and ancient Egyptians had cultivated flax and used its fibers to weave clothing. "Mummy" in various parts of Egypt were also covered with linen. Flax for oil is also called flax. Flax has been cultivated in my country for at least 1,000 years. Fiber type flax was introduced from Japan in 1906. China is mainly distributed in Heilongjiang and Jilin provinces. Linen likes cool, humid climates. Flax fiber has the characteristics of strong tensile force, softness, good fineness, weak conductivity, fast water absorption and dispersion, and high expansion rate....

Claims

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Application Information

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IPC IPC(8): C12N1/20D01C1/04D06M16/00C12S11/00C12R1/085D06M101/06
Inventor 丁若垚董政娥郁崇文张兴群
Owner DONGHUA UNIV
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