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Eupenicillium javanicum DB4 strain as well as preparation and application thereof

A technology of Penicillium penicillium and strains, applied in biochemical equipment and methods, fungi, textiles and papermaking, etc., which can solve the problems of high energy consumption in chemical treatment processes, large equipment loss, and inability to recycle waste water.

Inactive Publication Date: 2012-01-25
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical treatment process consumes a lot of energy, equipment loss is large, and the discharged wastewater cannot be recycled, causing serious pollution problems

Method used

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  • Eupenicillium javanicum DB4 strain as well as preparation and application thereof
  • Eupenicillium javanicum DB4 strain as well as preparation and application thereof
  • Eupenicillium javanicum DB4 strain as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Selection of Flax Degumming Strains

[0043] (1) Sampling rotten seaweed from the Zhoushan area of ​​the East China Sea, first place the seaweed and the enrichment medium at a ratio of 1g:20ml in the enrichment medium for 30 days at room temperature, then take 0.1ml of the enrichment medium and apply it to the separation Culture medium at 30℃ for 1 to 4 days to obtain dominant strains of wild-type flax biological treatment;

[0044] Among them, the enrichment medium formula is: flax meal 5g, tap water 200ml, pH is natural; the separation medium formula is: flax meal 5g, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 7-7.2 with NaOH;

[0045] (2) Inoculate the strain obtained in step (1) into a flax lignin nutrient medium, and cultivate for 72 hours at 28°C. Flax lignin nutrient medium, its components include: Flax lignin 2g, NaNO 3 3g, K 2 HPO 4 0.5g, MgSO 4 1g, 20g agar, 1000mL water, adjust the pH to 7-7.2 wit...

Embodiment 2

[0048] Preparation of fermentation broth:

[0049] (1) The DB4 strain of Eupenicillium javanicus obtained in Example 1 was stored in potato dextrose agar medium, cultured at 30°C at 220 rpm for 48 hours, and cryopreservation buffer was added; the cryopreservation buffer was composed of 0.0627g potassium dihydrogen phosphate, phosphoric acid It is prepared by 0.0177g dipotassium hydrogen, 0.0588g sodium citrate, 0.02645g magnesium sulfate heptahydrate, 10ml glycerin, and dilute to 100ml with water;

[0050] (2) In 50mL potato dextrose culture medium after sterilization and cooling, connect to the bacteria ring obtained in step (1), and culture in shake flask for 48h at a rotation speed of 220r / min and a temperature of 30℃;

[0051] (3) Inoculate the medium obtained in step (2) into 5L of flax lignin fermentation medium, shake flask culture for 48h at a rotation speed of 220r / min and a temperature of 30℃ to obtain a fermentation broth; wherein the formula of the flax fermentation mediu...

Embodiment 3

[0053] The flax degumming process is as follows:

[0054] Put 5g of the original flax stem into a 250ml Erlenmeyer flask, and divide the original flax stem and the fermentation broth of Example 2 into the Erlenmeyer flask at a bath ratio of 1:20. The filling volume is 100ml. The flask is placed in a shaker at 40°C and 200 rpm. Respectively degumming for 1 day; when the degumming time is up, remove the degumming solution and stop biological treatment. The biologically treated flax stems are inactivated, and the process is as follows: the biologically treated flax stems and the degumming solution are mixed at a ratio of 1:10, and treated at 70°C for 3 hours, in which peroxide The dosage is 3% of the original flax stem, the dosage of urea is 0.05% of the original flax stem, the dosage of sodium tripolyphosphate is 2% of the original flax stem, and the dosage of disodium ethylenediaminetetraacetic acid is 0.1% of the original flax stem. . 2,130 textile flax fibers can be made after...

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Abstract

The invention relates to a Eupenicillium javanicum DB4 strain as well as preparation and application thereof. The preparation method comprises the following steps of: (1) placing rotten seaweed in an enrichment culture medium, standing and culturing at room temperature, coating the enrichment culture medium on a separation culture medium, and standing and culturing at the temperature of 30 DEG C for 1-4 days to obtain wild flax bio-treated strain; (2) inoculating the strain obtained in the step (1) to a flax lignin nutrient culture medium and culturing at the temperature of 28 DEG C for 72 hours; and (3) selecting the strain with lignin degrading capability from the flax lignin nutrient culture medium. The obtained strain can be applied to the processes for preparing flax, hemp, jute or red ramie fibers for textile by peroxide degumming. The strain has the advantages of short growth period, low pollution probability, low treatment cost, mild reaction conditions, strong pollution resistance, good heat resistance, no environmental pollution, and good fiber quality after treatment; and the process is simple and is suitable for large-scale industrial production.

Description

Technical field [0001] The invention belongs to the field of Penicillium strains and preparation and application thereof, and particularly relates to a DB4 strain of Penicillium javanicus and preparation and application thereof. Background technique [0002] Hemp is one of the raw materials for textiles, and its varieties mainly include flax, ramie, hemp, jute, kenaf, apocynum, sisal, abaca, nettle and abutilon. Hemp fiber is a general term for fibers obtained from various hemp plants. It has the advantages of moisture absorption, ventilation, heat dissipation and antibacterial properties that other natural fibers cannot match. As one of the countries with the richest hemp resources in the world, my country produces hemp fiber and its products each year to meet the needs of the domestic market, and is also exported to the United States, Western Europe and Southeast Asian countries as a bulk commodity, earning nearly 10 billion U.S. dollars in foreign exchange annually. Driven by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14D01C1/00C12R1/645
Inventor 丁若垚刘国亮董政娥薛小华郁崇文张兴群
Owner DONGHUA UNIV
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