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Method for producing beta-mannase by bacillus germs

A technology of halobacterium bacillus and mannanase, which is applied in the field of bacterial production of β-mannanase, achieving the effects of short cycle, high enzyme activity and low cost

Inactive Publication Date: 2015-10-07
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the production of β-mannanase by bacteria of this genus

Method used

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  • Method for producing beta-mannase by bacillus germs

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specific Embodiment approach 1

[0018] Specific embodiment one: the method for producing β-mannanase by bacteria of the genus Halobacterium bacillus in this embodiment is carried out according to the following steps:

[0019] 1. Inoculate the bacteria HDYM-27 of the genus Sporohalobacter sp. in the konjac flour medium, so that the initial cell concentration in the medium is (1.0~2.0)×10 7 a / mL;

[0020] 2. Cultivate for 36-60 hours at a temperature of 32-38° C. to obtain a fermented liquid, and extract the fermented liquid to obtain β-mannanase.

[0021] After the fermentation, the β-mannanase activity in the fermentation broth is 2000-3000U / mL. The method has the advantages of short fermentation period, low cost and simple operation; the composition of the fermentation liquid is simple, the β-mannanase is easy to purify, and the fermentation liquid has no pollution to the environment. The fermented liquid is used for flax degumming with short time, complete removal of gum, low residual gum rate, high yiel...

specific Embodiment approach 2

[0023] Specific embodiment two: this embodiment is different from specific embodiment one in that: the konjac flour culture medium described in step one is made of 2g konjac flour, 0.5g ammonium nitrate, 0.5g sodium chloride and 100mL tap water. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0024] Embodiment 3: This embodiment is different from Embodiment 2 in that: the pH value of the konjac flour culture medium is 6.4-7.4. Others are the same as in the second embodiment.

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Abstract

The invention provides a method for producing beta-mannase by bacillus germs, and relates to a method for producing the beta-mannase by germs. The invention aims at expanding the resources of microbial strains for producing the beta-mannase, and provides a novel method for producing the beta-mannase by germs. The method comprises the following steps of: 1, inoculating the bacillus germs HDYM-27 into a konjaku flour culture medium; 2, culturing the germs under the condition of the temperature being 32 to 38 DEG C to obtain fermentation liquid, wherein the fermentation liquid contains the beta-mannase. The konjaku flour culture medium is prepared from konjaku flour, ammonium nitrate, sodium chloride and tap water. The beta-mannase activity in the fermentation liquid is 2000 to 3000 U / mL. The method has the advantages that the fermentation period is short, the cost is low, the operation is simple and convenient; ingredients of the fermentation liquid are simple, the beta-mannase can be easily purified, and the fermentation liquid has no pollution on the environment. The method is used for producing the beta-mannase.

Description

technical field [0001] The invention relates to a method for bacteria to produce β-mannanase. Background technique [0002] Sporohalobacter sp. is a spore-forming bacterium that can live anaerobically in a high-salt environment. The genus of bacteria contains two species, namely S. lortetii and S. marimortui. There is no report of producing β-mannanase from this genus of bacteria. [0003] β-mannanase, English name β-mannanase, is an endohydrolase that hydrolyzes the main chain of 1,4-β-D-mannopyranose. β-Mannanase has a wide range of substrates including glucomannan, mannan and galactomannan. β-Mannanase has a wide range of industrial applications, such as pulp bleaching in the paper industry, fruit juice clarification in the food industry, etc. β-mannanase degrades polysaccharides into oligosaccharides, which is beneficial to the growth of intestinal beneficial microorganisms, and has attracted increasing attention in recent years. Microbial β-mannanase has a wide ran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12R1/01
CPCC12N9/2494C12Y302/01078
Inventor 葛菁萍赵丹平文祥杜仁鹏张历王琪
Owner HEILONGJIANG UNIV
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