Bacillus subtilis capable of simultaneously producing pectase and hemicellulase, screening method and applications thereof
A technology of Bacillus subtilis and hemicellulase, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of less research on hemp fiber bio-degumming and too long hemp fiber processing cycle
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[0040] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.
[0041] Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. For those skilled in the art, on the premise of not departing from the spirit and scope of the present invention, various changes or modifications to the material components and dosage in these embodiments also belong to the protection scope of the present invention.
[0042] The culture medium used in the present invention is as follows:
[0043] Hemp enrichment medium: 2.0g of hemp powder, 0.2g of ammonium nitrate, 0.05g of dipotassium hydrogen phosphate, 0.05g of sodium sulfate, 100mL of distilled water, pH7.0.
[0044] Pectin screening medium: 0.5g pectin, 0.2g ammonium nitrate, 0.1g dipotassium hydrogen phosphate, 0.05g...
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