Bacillus subtilis capable of simultaneously producing pectase and hemicellulase, screening method and applications thereof

A technology of Bacillus subtilis and hemicellulase, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of less research on hemp fiber bio-degumming and too long hemp fiber processing cycle

Inactive Publication Date: 2016-05-11
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] At present, domestic and foreign researches on the biological degumming of hemp mostly focus on the biological degumming of ramie, flax,

Method used

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  • Bacillus subtilis capable of simultaneously producing pectase and hemicellulase, screening method and applications thereof
  • Bacillus subtilis capable of simultaneously producing pectase and hemicellulase, screening method and applications thereof
  • Bacillus subtilis capable of simultaneously producing pectase and hemicellulase, screening method and applications thereof

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Embodiment Construction

[0040] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0041] Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. For those skilled in the art, on the premise of not departing from the spirit and scope of the present invention, various changes or modifications to the material components and dosage in these embodiments also belong to the protection scope of the present invention.

[0042] The culture medium used in the present invention is as follows:

[0043] Hemp enrichment medium: 2.0g of hemp powder, 0.2g of ammonium nitrate, 0.05g of dipotassium hydrogen phosphate, 0.05g of sodium sulfate, 100mL of distilled water, pH7.0.

[0044] Pectin screening medium: 0.5g pectin, 0.2g ammonium nitrate, 0.1g dipotassium hydrogen phosphate, 0.05g...

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Abstract

The invention relates to a strain of bacillus subtilis capable of simultaneously producing pectase and hemicellulase, a screening method and applications thereof. The strain is named as 16#, and belongs to bacillus subtilis. The preservation number is CGMCC No.11898, the preservation date is 22nd December, 2015, and the preservation organization is China General Microbiological Culture Collection Center (CGMCC), whose address is Beichenxi Road No.1 Yard No.3, Chaoyang District, Beijing. The provided bacillus subtilis is capable of simultaneously producing pectase and hemicellulase, barely produces cellulase, and can be applied to degumming of hemp. The bacillus subtilis is applied to degumming at a room temperature for 96 hours or so, the rate of residual gum can reach 13% or so, the processed hemp can almost reach the requirements of textile industry, and the provided bacillus subtilis can also be applied to degumming of other hemps.

Description

technical field [0001] The invention belongs to the technical field of industrial microbes, in particular to a bacillus subtilis capable of simultaneously producing pectinase and hemicellulase, a screening method and application thereof. Background technique [0002] Cannabis is an annual Moraceae plant of the genus Cannabis, with a height of about 3 meters. The scientific name is Cannabissatival, also known as hemp, cold hemp, thread hemp, flower hemp, etc. Hemp cultivation has very low requirements on climate and soil, and there is no need for pesticides or external fertilization during the growth process. It has strong insect resistance, rapid growth, and short planting and harvesting period. The most important thing is that hemp fiber has many unique excellent properties. Such as anti-static, anti-allergic, good thermal insulation, heat dissipation, anti-bacterial, anti-corrosion, anti-ultraviolet, etc. Hemp fiber is an important textile fiber crop. [0003] Compared ...

Claims

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Application Information

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IPC IPC(8): C12N1/20D01C1/04C12R1/125
CPCC12N1/20C12N1/205C12R2001/125D01C1/04
Inventor 黎明路福平杨田
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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