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Method for extracting chlorogenic acid from honeysuckle by using compound enzyme method

A compound enzyme method and chlorogenic acid technology, applied in the separation/purification of carboxylic acid esters, organic chemistry, etc., can solve the problems of cumbersome operation, popular use, large investment, environmental pollution, etc.

Inactive Publication Date: 2010-09-15
ZHONGXIN PHARMA FACTORY OF TIANJIN ZHONGXIN PHARMA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction techniques of chlorogenic acid in honeysuckle currently reported in the literature mainly include: water decoction, acid-base extraction, ethanol reflux, etc., using simple physical methods to extract the active ingredient chlorogenic acid in honeysuckle, but the above methods exist However, the extraction rate is low, the cost is high, and it causes environmental pollution; and recently, the use of ultrasonic method, supercritical CO 2 The method of extracting chlorogenic acid with new physical instruments such as extraction has attracted widespread attention due to its high extraction efficiency, but due to problems such as excessive investment and cumbersome operation in production, the popularization and use of the above method in industrial production is limited.

Method used

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  • Method for extracting chlorogenic acid from honeysuckle by using compound enzyme method
  • Method for extracting chlorogenic acid from honeysuckle by using compound enzyme method
  • Method for extracting chlorogenic acid from honeysuckle by using compound enzyme method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Accurately weigh 5 g of honeysuckle medicinal material powder through a No. 4 sieve, and then dissolve the compound enzyme in an aqueous solution of 10 times the amount of medicinal material powder for subsequent use;

[0076] (2) Add compound enzyme according to 200U / g medicinal material (U is activity unit), control enzymolysis temperature at 40°C, pH value 4.5, enzymolysis time 1.5 hours, and process honeysuckle; wherein the compound ratio of cellulase and pectinase is 1.0:0.3.

[0077] (3) After the enzymatic hydrolysis is completed, add water at 70°C in an amount 10 times the weight of the medicinal material, extract twice, each time for 2 hours, combine the leachate, and concentrate under reduced pressure;

[0078] (4) Dissolve the concentrated solution in 50% methanol solution, set the volume to 50mL, accurately draw 1mL and use 50% methanol solution to set the volume to 100mL, measure the content of chlorogenic acid by HPLC method to be 2.34%, and calculate ...

Embodiment 2

[0080] (1) Accurately weigh 5 g of honeysuckle medicinal material powder through a No. 4 sieve, and then dissolve the compound enzyme in an aqueous solution of 10 times the amount of medicinal material powder for subsequent use;

[0081] (2) Add compound enzyme according to 400U / g medicinal material (U is activity unit), control enzymolysis temperature at 45°C, pH value 5, enzymolysis time 2 hours, process honeysuckle; wherein the compound ratio of cellulase and pectinase is 1.0:0.28.

[0082] (3) After the enzymatic hydrolysis is completed, add water at 75°C in an amount 10 times the weight of the medicinal material, extract 3 times, each time for 3 hours, combine the leachate, and concentrate under reduced pressure;

[0083] (4) Dissolve the concentrated solution in 50% methanol solution, set the volume to 50mL, accurately draw 1mL and use 50% methanol solution to set the volume to 100mL, measure the content of chlorogenic acid by HPLC method to be 2.34%, and calculate the e...

Embodiment 3

[0085] (1) Accurately weigh 5 g of honeysuckle medicinal material powder through a No. 4 sieve, and then dissolve the compound enzyme in an aqueous solution of 10 times the amount of medicinal material powder for subsequent use;

[0086] (2) Add compound enzyme according to 400U / g medicinal material (U is activity unit), control enzymolysis temperature at 40°C, pH value 4.5, enzymolysis time 2 hours, process honeysuckle; wherein the compound ratio of cellulase and pectinase is 1.0:3.

[0087] (3) After the enzymatic hydrolysis is completed, add water at 70°C in an amount 10 times the weight of the medicinal materials, extract twice, each time for 3 hours, combine the extracts, and concentrate under reduced pressure;

[0088] (4) Dissolve the concentrated solution in 50% methanol solution, set the volume to 50mL, accurately draw 1mL and use 50% methanol solution to set the volume to 100mL, measure the content of chlorogenic acid by HPLC method to be 2.34%, and calculate the ext...

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Abstract

The invention relates to a method for extracting chlorogenic acid from honeysuckle by using a compound enzyme method and researches a novel process for extracting a main medicinal effective component of the chlorogenic acid from the honeysuckle by a cellulose and pectinase compounding enzyme method. Through a single factor experiment and an orthogonal test, optimal process conditions for extracting the chlorogenic acid from the honeysuckle by using the compound enzyme method can be optimized: the enzymolysis temperature is 45DEG C, the pH is 4.5, the compound ratio of the cellulose to the pectinase is 1.0:0.3 and the enzymolysis time is 1.5 hours. The test result shows that the process repeatability is favorable and the result is stable. The extraction rate of the chlorogenic acid extracted from the honeysuckle by using the compound enzyme method can reach 83.06 percent which is improved by 16.93 percent in comparison with the extraction rate of the chlorogenic acid extracted by using a water extraction process.

Description

technical field [0001] The invention belongs to the technical field of extraction of traditional Chinese medicinal materials, and relates to a new process for extracting chlorogenic acid, an effective medicinal component, from honeysuckle with functions of clearing away heat, detoxification, antibacterial and anti-inflammation. More specifically, the optimal process for extracting chlorogenic acid from Lonicerae japonica by compound enzymatic method was optimized through single factor test and orthogonal test. Background technique [0002] Lonicera japonica Thunb is the dry flower bud or the first flower of Lonicera japonica Thunb. It is one of the commonly used traditional Chinese medicines for clearing away heat, detoxification, antibacterial and anti-inflammation (Lu Xufang. Research status of honeysuckle[J]. Shi Zhen Guo Yi Guo Yao , 2006, 17(5): 843-844). Its chemical components are chlorogenic acid, isochlorogenic acid, volatile oil, caffeic acid, flavonoids, triterpe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48
Inventor 凌宁生郑丽娜王岩
Owner ZHONGXIN PHARMA FACTORY OF TIANJIN ZHONGXIN PHARMA GROUP
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