Alkaline pectinase production method and application in papermaking pulping

Abstract

The invention discloses an alkaline pectinase production method and application in papermaking pulping. The alkaline pectinase production method comprises the steps of: selective breeding of a bacterial strain, culturing of the bacterial strain, fermentation through shaking a flask, and preparation of an enzyme liquid. The method is characterized in that the bacterial strain subjected to the selective breeding is achieved through separated screening of a naturally acquired bacterial sample and mutagenesis of the bacterial strain at cellular level, i.e. heat-resistant Bacillussubtillis with a laboratory number of MAPLE61 and preservation number of CCTCC NO:M2010004; and when the heat-resistant Bacillussubtillis is used for papermaking pulping, raw materials are soaked with an alkaline pectinase solution after the pickling treatment. When the invention is used for papermaking pulping, the prepared chemical pulp has favorable strength property and bleaching property; and after simple oxidation chlorine bleaching, the whiteness of the pump can reach 80-90 percent, breaking length can reach 6000-7000m, and meanwhile, alkali consumption is reduced about 20-40 percent and the production cost is reduced about 20 percent.

Description

technical field [0001] The invention relates to a pectinase and a papermaking and pulping process, in particular to a production method of alkaline pectinase and its application in papermaking and pulping. Background technique [0002] Modern papermaking technology has a history of hundreds of years, but the progress of papermaking technology is very slow. The traditional chemical pulping process, such as caustic soda method, sulfate method, etc., has the disadvantages of high temperature, high pressure, high energy consumption, low yield, damage to fiber strength, etc., not only high production cost, but also has certain safety hazards. A large amount of black liquor is also produced during the production process, which has the characteristics of high viscosity, low organic matter content, high silicon content, and low calorific value, making it difficult to effectively recycle and causing serious environmental pollution. Biopulping technology is considered to be one of th...

AI Technical Summary

Problems solved by technology

[0003] Using microorganisms to directly process raw materials, such as the publication number CN1683711A, the name of the invention is a papermaking biological pulping process; the publication number is CN1420229A, the name of the invention is a papermaking straw pulping process; A papermaking process method that replaces the chemical pulping process with a biological fermentation pulping method; the publication number is CN1188830A, and the invention name is biological delignification-mechanical pulping technology and other patent applications are all using microorganisms to directly process raw materials, and their main disadvantages are as follows: : ⑴The cycle of biological treatment is long and usually takes a few days; ⑵The raw materials should be sterilized before microbial treatment, which increases the production cost and may reduce the quality of raw materials; ⑶It is difficult to effectively control the biological treatment process, and often occurs The poor quality uniformity of raw materials and serious carbohydrate degradation lead to a decrease in pulping yield; (4) The poor quality uniformity of raw materials after biological treatment also directly affects the quality of pulping, thereby affecting the quality of the final product; (5) Produced by the above method Compared with ordinary chemical pulp, pulp has poor physical properties and bleaching properties, and cannot be used to produce high-brightness, high-strength paper, but can only be used to produce low-grade paper

[0004] Using enzymes such as ligninase, xylanase, and hemicellulase to treat raw materials, such as the publication number CN1616758A, the name of the invention is biological pulping process; the publication number is CN1421570A, and the name of the invention is enzymatic pulping of grass raw materials method and other patents, the main disadvantages of this process are: the cost of the enzyme solution is high, and it does not have a cost advantage in industrial production; at the same time, it is difficult to control the destruction of the corresponding components by ligninase, xylanase, and hemicellulase, which may Excessive removal of lignin, xylan, and hemicellulose affects pulp yield