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Bacterial strain producing phloem fibre degumming enzyme and its application in ramie and hemp degumming

A bast fiber and degumming enzyme technology, applied in the direction of bacterial retting, enzymes, bacteria, etc., can solve the problems of no hemp degumming, low pectinase activity, and difficult application

Inactive Publication Date: 2002-10-02
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent 97109044 discloses a strain of alkalophilic bacillus and its ramie degumming method, but because the pectinase activity it produces is relatively low and the degumming time is long (16~24h.), it is also difficult to apply in industry
None of the above patents involves the degumming of marijuana

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] CCTCC No.M200038 strains were kept for later use or the newly cultivated slant strains were inoculated into the enzyme-producing medium in shake flasks. The preparation method is as follows: 50 ml of the enzyme-producing medium in a 300-ml shaker bottle is placed at 32° C.±1° C., and cultured in a shaker at 200 r / min for 72 hours. The formula of the enzyme production medium is: 3.0 parts of bran, 1.0 part of corn flour, 0.5 part of bean cake flour, (NH 4 ) 2 SO 4 1.0 servings, K 2 HPO 4 0.1 part, MgSO 4 ·7H 2 0.1 part of O, 100 parts of water, PH8.0 before elimination. After culturing, remove the shake flask, filter the fermentation broth with 4 layers of gauze or centrifuge at 2000-3000 r / min, take the clear liquid, measure the enzyme activity according to Somogyi's method, pectinase (with Sigma polygalacturonic acid as the bottom) The activity of xylanase (using Sigma xylan as substrate) was 61 μ / ml, and the activity of mannanase (using Sigma locust bean gum as...

Embodiment 2

[0013] After the enzyme solution produced in Example 1 was diluted 5 times, the pH was adjusted to 8.5, and the bath ratio of the diluted enzyme solution and cannabis was 10~16:1 (volume:weight), placed in a water bath of 40°C to 50°C for thermal insulation and degumming for 4h, and taken out. After washing, it was sent to Shandong Fiber Inspection Bureau for measurement. The main index results were: fiber fineness 12.9-18.25dtex, fiber breaking strength 3.07-3.14CN / dtex, and elongation 2.5-1.9CV%.

Embodiment 3

[0015] Bacteria CCTCC No.M200038 was expanded in the fermenter to cultivate the cells and produce enzymes and applied to ramie and hemp degumming.

[0016] The process flow of fermenter expansion and cultivation to produce degumming enzyme is: slant strain → shake flask strain → 50L seed tank → 500L seed tank → 10M 3 Fermenter

[0017] The conditions for expanding the production of degumming enzymes in the fermenter are: ① 50L, 500L and 10M 3 The fermenter medium formula is: 3.0 parts of bran, 1.0 part of corn flour, 0.5 part of bean cake flour, (NH 4 ) 2 SO 4 1.0 servings, K 2 HPO 4 0.10 parts, MgSO 4 ·7H 2 O0.10 parts, eliminate the pre-adjustment PH7.5~8.5, ②50L, 500L and 10M 3 Fermentation tank control conditions, tank pressure 0.03Mpa, temperature 32 ℃ ~ 34 ℃, ventilation 1:0.5 ~ 0.8, culture time 50L tank 12h, 500L tank 10h, 10M 3 Tank, 18h, ③ pectinase activity was 508μ / ml, the final pH of the fermentation broth was 5.5, no peculiar smell, the cells grew well...

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Abstract

The present invention relates to a strain capable of producing bast fiber degumming enzyme and its application for degumming ramie and hemp, belonging to the field of microbial technology. Said strain is bacillus subtilis No.13, and its preservation number is CCTCC, No.M200038. Its degumming rate can be up to 95%, its degumming effect is good, and its degumming process can be completed within 2-6hr., and the fibre separation index is 100%.

Description

(1) Technical field [0001] The invention relates to a strain producing bast fiber degumming enzyme and its application in degumming of ramie and hemp. (2) Technical background [0002] For more than half a century, people have tried to change the environmental pollution caused by the chemical degumming process and the partial deterioration of the textile properties of hemp fibers. Therefore, many researchers in this field are trying to find a new method of hemp degumming. Biological degumming is subject to particular attention. In 1953, Liu Qisong, Shenyang Institute of Forestry and Soil Research, Chinese Academy of Sciences, etc. conducted research on the ramie degumming microbial flora. Chaudhurg.S.D. reported that some bacteria and fungi can decompose pectin substances and release fibers during the fermentation process. In 1958 Year Ali (Ali.M.M.) proposed the use of pectinase for degumming of hemp (such as jute, flax, etc.) fibers. In 1972, Fogarty W.M. and Ward D.D. r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/00C12S3/06D01C1/04
Inventor 刘自鎔任建平冯瑞良张桂珍
Owner SHANDONG UNIV
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