Method for extracting leaf protein from alfafa hay

A technology of alfalfa and leaf protein is applied in the direction of animal feed, additional food elements, animal feed, etc. It can solve the problems of idle production equipment and waste, and achieve the effects of saving investment, low cost and reducing extraction time.

Inactive Publication Date: 2011-09-14
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of leaf protein generally uses fresh alfalfa as raw material, but because the nutrient content of forage is closely related to the growth period and growing se...

Method used

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  • Method for extracting leaf protein from alfafa hay
  • Method for extracting leaf protein from alfafa hay
  • Method for extracting leaf protein from alfafa hay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Crush air-dried or dried alfalfa with a moisture content of less than 15%, pass through a 60-mesh sieve, weigh 10g of alfalfa powder, add 2% of the weight of alfalfa powder, that is, 0.2g, and then add 10 times the weight Soak in grass powder water for 12h.

[0019] (2) Beat the pulp for 5 minutes, filter or centrifuge with a 120-mesh filter cloth to obtain green juice.

[0020] (3) Adjust the pH value of the green juice to 3 with 0.5mol / L sodium hydroxide and 0.5mol / L hydrochloric acid, quickly put it into a boiling water bath and heat it to 60°C, then transfer it to a constant temperature water bath at the same temperature for flocculation for 10 minutes.

[0021] (4) Quickly cool to room temperature with cold water, let it stand for 1 hour, filter or centrifuge to obtain leaf protein paste, and dry it in an oven at 60°C-70°C to obtain the finished leaf protein, which is stored in a sealed container. The yield of leaf protein can reach 8.87%, and the purity can r...

Embodiment 2

[0024] (1) Crush air-dried or dried alfalfa with a moisture content of less than 15%, pass through a 60-mesh sieve, weigh 10g of alfalfa powder, add 1% of the weight of alfalfa powder, that is, 0.1g, and then add 20 times the weight Soak in grass powder water for 24 hours.

[0025] (2) Beat the pulp for 5 minutes, filter or centrifuge with a 120-mesh filter cloth to obtain green juice.

[0026] (3) Adjust the pH value of the green juice to 3.5 with 0.5moL / L sodium hydroxide and 0.5moL / L hydrochloric acid, quickly put it in a boiling water bath and heat it to 60°C, then transfer it to a constant temperature water bath at the same temperature for flocculation for 10 minutes.

[0027] (4) Quickly cool to room temperature with cold water, let it stand for 1 hour, filter or centrifuge to obtain leaf protein paste, and dry it in an oven at 60°C-70°C to obtain the finished leaf protein, which is stored in a sealed container. The highest yield of leaf protein can reach 11.48%, and th...

Embodiment 3

[0030] (1) Crush air-dried or dried alfalfa with a moisture content of less than 15%, pass through a 60-mesh sieve, weigh 10g of alfalfa powder, add 1% of the weight of alfalfa powder, that is, 0.1g, and then add 30 times the weight Soak in grass powder water for 24 hours.

[0031] (2) Beat the pulp for 5 minutes, filter or centrifuge with a 120-mesh filter cloth to obtain green juice.

[0032] (3) Adjust the pH value of the green juice to 3 with 0.5moL / L sodium hydroxide and 0.5moL / L hydrochloric acid, quickly put it in a boiling water bath and heat it to 60°C, then transfer it to a constant temperature water bath at the same temperature for flocculation for 15 minutes.

[0033] (4) Quickly cool to room temperature with cold water, let it stand for 1 hour, filter or centrifuge to obtain leaf protein paste, and dry it in an oven at 60°C-70°C to obtain the finished leaf protein, which is stored in a sealed container. The highest yield of leaf protein can reach 11.52%, and the ...

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Abstract

The invention relates to a method for extracting leaf protein from alfafa hay, aiming at providing a preparation method which can realize mass production and has the advantages of no time limit, simple process, low cost and high efficiency. In the method provided by the invention, leaf protein is extracted by acidification heating method and salt treatment. The method comprises the following steps of: smashing air-dried or kiln-dried alfalfa, adding appropriate amount of sodium pyrosulphate, adding water in the ratio of material to water being 1: (10-40), preimpregnating for 12-36 hours and then beating for 5 minutes, filtering (or centrifuging) with a 120-mesh filter cloth to obtain green juice, adjusting pH value to 2-7 by utilizing 2% sodium hydroxide and 0.5 mol/L hydrochloric acid solution, rapidly heating to the temperature of 40-80 DEG C in a boiling water bath, and then flocculating for 3-20 minutes in a constant-temperature water bath at the same temperature; and rapidly cooling to room temperature with cold water (normal temperature), standing for 1 hour, filtering (or centrifuging) to obtain leaf protein paste, and finally drying in a drying oven at the temperature of 60-70 DEG C. In the extraction method, the leaf protein is extracted from alfafa hay used as raw material; compared with the traditional method which takes fresh alfafa hay as the raw material, the cost is reduced, the yield efficiency is greatly improved, and the defect that the production time is limited by plant growth season is overcome.

Description

technical field [0001] The invention belongs to a method for extracting plant active components, in particular to a method for extracting leaf protein from alfalfa hay. Background technique [0002] Insufficient protein resources are a worldwide problem. Leaf protein is the most abundant protein on earth, and its development and utilization have attracted the attention of countries all over the world. The protein yield per unit area of ​​green leaves is generally 2-4 times that of food crops, and is known as the largest protein source in the world. Leaf protein or green protein concentrate (Leaf protein concentration, referred to as LPC) is extremely nutritious, its protein content is generally 45-65%, rich in 18 kinds of amino acids, lutein and polysaccharides, and also contains carotene, carbohydrates, Minerals, vitamin E, vitamin C, group B vitamins and unknown growth-promoting factors, etc., the proportion of amino acid components is reasonable, the taste is good, and ...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23K1/14A23K1/16A23K10/30A23K20/147
Inventor 朱宇旌张勇宋执磊
Owner SHENYANG AGRI UNIV
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