Glutamic acid high-yielding technology

A glutamic acid and process technology, which is applied in the field of amino acid fermentation, can solve the problems of low conversion rate and high cost of fermentation medium, and achieve the effects of improved strain utilization, reasonable compatibility, and increased industrial added value

Active Publication Date: 2016-09-07
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the invention is to solve the defects of high cost and low conversion rate of the fermentation medium in the prior art, and provide a process for high glutamic acid production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of glutamic acid high yield acid technique, it comprises the steps:

[0023] 1) Take various raw materials according to weight percentage, including: 5% wheat bran hydrolyzate, 3% glucose, 2% straw treatment, 1% rice bran extract, 0.05% urea, 0.02% shell powder, 0.02% magnesium sulfate, phosphoric acid Potassium dihydrogen 0.01%, the rest is water;

[0024] 2) Add wheat bran hydrolyzate, glucose, straw treatment, rice bran extract, urea, shell powder, magnesium sulfate and potassium dihydrogen phosphate to water in sequence, stir evenly, and then extinguish at a temperature of 108°C for 8 minutes. Bacteria treatment, and then lower the temperature to 32°C to obtain a fermentation medium;

[0025] 3) The seed culture solution of the glutamic acid temperature-sensitive strain Brevibacterium flavum TMG0106 in the logarithmic growth phase was inserted into the fermentation medium at a volume ratio of 8% of the seed culture medium: fermentation medium, and the fermen...

Embodiment 2

[0034] A kind of glutamic acid high yield acid technique, it comprises the steps:

[0035] 1) Take various raw materials according to weight percentage, including: 9% wheat bran hydrolyzate, 5% glucose, 3% straw treatment, 2% rice bran extract, 0.08% urea, 0.03% shell powder, 0.03% magnesium sulfate, phosphoric acid Potassium dihydrogen 0.02%, the rest is water;

[0036] 2) Add wheat bran hydrolyzate, glucose, straw treatment, rice bran extract, urea, shell powder, magnesium sulfate and potassium dihydrogen phosphate to water in sequence, stir evenly, and then extinguish at a temperature of 110°C for 10 minutes. Bacteria treatment, and then lower the temperature to 32°C to obtain a fermentation medium;

[0037] 3) Put the glutamic acid temperature-sensitive strain Brevibacterium flavum CN1021 seed culture solution in the logarithmic growth phase into the fermentation medium at a volume ratio of 8-10% of the seed culture medium: fermentation medium, and control the fermentatio...

Embodiment 3

[0046] Comparative test of process acid production rate and sugar-acid conversion rate of the present invention:

[0047] Control group 1: Fermentation medium uses: glucose 3-5%, yeast extract 4-7%, peptone 5-9%, corn steep liquor 1-2%, magnesium sulfate 1-2%, urea 0.05-0.08%, sulfite Iron 0.02-0.03%, magnesium sulfate 0.02-0.03%, potassium dihydrogen phosphate 0.01-0.02%, VB1 0.01-0.02%, VH 0.01-0.02%, and the rest is water; the fermentation process is the same as in Example 1.

[0048] Control group 2: Fermentation medium used: glucose 3-5%, yeast extract 4-7%, peptone 5-9%, corn steep liquor 1-2%, magnesium sulfate 1-2%, urea 0.05-0.08%, sulfite Iron 0.02-0.03%, magnesium sulfate 0.02-0.03%, potassium dihydrogen phosphate 0.01-0.02%, VB1 0.01-0.02%, VH 0.01-0.02%, and the rest is water; the fermentation process is the same as in Example 2. The acid production rate and sugar-acid conversion rate of each group are shown in Table 1:

[0049] Table 1

[0050] group ...

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Abstract

The invention belongs to the technical field of amino acid fermentation, and discloses a glutamic acid high-yielding technology. Glutamic acid is prepared through fermenting glutamic acid temperature sensitive strains through a fermentation culture by adopting a temperature sensing technology. The technology has the advantages of reasonable compatibility of the fermentation medium, low cost, high acid yielding efficiency, enterprise cost saving and income increase.

Description

technical field [0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to a process for high glutamic acid production. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. Sodium glutamate, commonly known as monosodium glutamate, is an important umami agent that enhances the fragrance. Sodium glutamate is widely used as a food flavoring agent, either alone or i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14
CPCC12P13/14
Inventor 董力青刘世周程士清尤学波
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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