Preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth

A technology for the use of Pseudomonas chloropinus, which is applied in the preparation of Pseudomonas chloropinus agents and the preparation of microbial preparations, can solve the problems of high residue, drug resistance, chemical pesticide pollution, etc., and achieve environmental friendliness , good stability, and powerful biological control functions

Active Publication Date: 2014-09-03
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to overcome the serious pollution in the production process of chemical pesticides for the prevention and treatment of plant fungal diseases in the above-mentioned prior art, the high residues in soil, water and crops, and the increasingly serious drug resistance, etc., to provide a disease-resistant Preparation method and application of growth-promoting Pseudomonas chloropinus agent

Method used

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  • Preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth
  • Preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth
  • Preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth

Examples

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Embodiment 1

[0037] 1. Acquisition and identification of Pseudomonas chloropinus HT66

[0038] First, select a vigorously growing rice plant without any visible lesions in a dry paddy field, remove the stem part of the rice and the 5 cm thick surface soil, and dig out a rhizosphere soil sample with a sterile medicine spoon; weigh 1 g Soil samples were placed in a 250mL Erlenmeyer flask filled with 50mL KMB medium, and ampicillin (final concentration was 50 μg / mL), chloramphenicol (final concentration was 15 μg / mL) and cycloheximide (final concentration The concentration is 100μg / mL) to reduce the contamination of fungi in the soil. Then place the flask on a shaking table with a rotating speed of 200rpm at 28°C for 2 hours; take a sample, dilute it 1000 times, and spread it on a solid KMB plate containing the same antibiotic, and use the asynchronous culture method to test the resistance of rice sheath blight to the single colony obtained. In the experiment of pathogenic bacteria, the stra...

Embodiment 2

[0059] The present embodiment prepares the Pseudomonas chloropinus agent on the basis of Example 1 and carries out biological assay to it; specifically as follows:

[0060] 1. Preparation of bacteria agent

[0061] Prepare solid KMB solid and liquid medium, inoculate the Pseudomonas chlorosinum HT66 frozen at -80°C on the solid medium, activate it at 28°C for 48 hours; then transfer to a new solid medium and continue passage 2 times; then pick a ring of activated bacteria, inoculate into 50mL liquid culture medium (packed in 250mL Erlenmeyer flask), place on a constant temperature shaker at 28°C for 16 hours, and shake at a speed of 180 rpm. Then, the seed solution was further transferred to 1000 mL of liquid medium, and cultivated under the same conditions; finally, a larger volume of liquid KMB medium was prepared, put into a fermenter and sterilized at 121°C for 20 minutes; When the temperature is cooled to 28°C, add the seed solution of Pseudomonas chloropinus at a ratio ...

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Abstract

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu/mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

Description

technical field [0001] The invention belongs to the field of biological pesticides and biological fertilizers, and relates to a preparation method and application of a disease-preventing and growth-promoting microbial preparation; in particular, it relates to a preparation method and application of a disease-resistant and growth-promoting Pseudomonas chloropina agent. Background technique [0002] The research results of the Food and Agriculture Organization of the United Nations show that the natural loss rate of crop diseases and insect pests is more than 37%, seriously affecting food security and environmental ecological security. According to the survey, there are 1,600 kinds of disease organisms in common crops in my country, causing losses of nearly 100 billion RMB every year, seriously hindering the development of my country's agriculture. At present, due to the extensive use of various chemical pesticides in agricultural production, many types of diseases and insect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C05F11/08C12R1/38
CPCY02E50/343Y02E50/30Y02W30/40
Inventor 彭华松张平原张雪洪王威胡洪波
Owner SHANGHAI JIAO TONG UNIV
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