Preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth
A technology for the use of Pseudomonas chloropinus, which is applied in the preparation of Pseudomonas chloropinus agents and the preparation of microbial preparations, can solve the problems of high residue, drug resistance, chemical pesticide pollution, etc., and achieve environmental friendliness , good stability, and powerful biological control functions
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Embodiment 1
[0037] 1. Acquisition and identification of Pseudomonas chloropinus HT66
[0038] First, select a vigorously growing rice plant without any visible lesions in a dry paddy field, remove the stem part of the rice and the 5 cm thick surface soil, and dig out a rhizosphere soil sample with a sterile medicine spoon; weigh 1 g Soil samples were placed in a 250mL Erlenmeyer flask filled with 50mL KMB medium, and ampicillin (final concentration was 50 μg / mL), chloramphenicol (final concentration was 15 μg / mL) and cycloheximide (final concentration The concentration is 100μg / mL) to reduce the contamination of fungi in the soil. Then place the flask on a shaking table with a rotating speed of 200rpm at 28°C for 2 hours; take a sample, dilute it 1000 times, and spread it on a solid KMB plate containing the same antibiotic, and use the asynchronous culture method to test the resistance of rice sheath blight to the single colony obtained. In the experiment of pathogenic bacteria, the stra...
Embodiment 2
[0059] The present embodiment prepares the Pseudomonas chloropinus agent on the basis of Example 1 and carries out biological assay to it; specifically as follows:
[0060] 1. Preparation of bacteria agent
[0061] Prepare solid KMB solid and liquid medium, inoculate the Pseudomonas chlorosinum HT66 frozen at -80°C on the solid medium, activate it at 28°C for 48 hours; then transfer to a new solid medium and continue passage 2 times; then pick a ring of activated bacteria, inoculate into 50mL liquid culture medium (packed in 250mL Erlenmeyer flask), place on a constant temperature shaker at 28°C for 16 hours, and shake at a speed of 180 rpm. Then, the seed solution was further transferred to 1000 mL of liquid medium, and cultivated under the same conditions; finally, a larger volume of liquid KMB medium was prepared, put into a fermenter and sterilized at 121°C for 20 minutes; When the temperature is cooled to 28°C, add the seed solution of Pseudomonas chloropinus at a ratio ...
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