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Method for preparing 6-gingerol and 8-gingerol from ginger

A technology of gingerol and ginger, which is applied in the field of natural active product preparation, can solve the problems of less preparation amount, lower yield, and low product yield, and achieve low requirements for instruments and equipment, avoid solvent extraction, and high product yield Effect

Inactive Publication Date: 2010-03-24
NO 411 HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process route of this method is long, and the high-efficiency liquid phase separation is easily limited by the instrument conditions, and the preparation amount is small, so it is not suitable for industrial production; the macroporous resin purification method has the disadvantage of yield reduction caused by adsorption
The above two methods are the separation and preparation of a single component of 6-gingerol, and the existing literature does not involve the process of preparing 6-gingerol and 8-gingerol simultaneously
[0007] As can be seen from the above, the existing preparation process steps are relatively long, the operation is cumbersome, and the product yield is not high. It can be used as the preparation of a small amount of products in the laboratory, but it is difficult to apply to large-scale production, and they are all 6-gingerol single products.

Method used

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  • Method for preparing 6-gingerol and 8-gingerol from ginger

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Wash 5kg of fresh ginger, cut into thin slices, dry below 60°C, pulverize in a grinder, and pass through a 100-mesh sieve. Put the above-mentioned gingerol in a 2L round-bottomed flask, add ethyl acetate at a ratio of ginger powder: ethyl acetate volume ratio of 1: 2 to 3, heat and reflux for extraction 3 times, each time for 2 hours, and combine the extracts, 40 Below ℃, use a rotary evaporator to depressurize and return to the extract, and the pressure is 0.08-0.1Mpa to obtain 250g of extract extract;

[0021] Dissolve the above extract with 400mL ethyl acetate, add 750g 200-300 mesh silica gel for column chromatography to mix the sample, heat in a water bath to evaporate the ethyl acetate, then add 5.0kg blank silica gel (200-300 mesh ) column, first elute with 10BV petroleum ether (60-90°C), then 8BV petroleum ether: ethyl acetate (20:1, V / V), and finally use petroleum ether: ethyl acetate volume ratio 10:1 elution, TLC detection elution progress (silica gel GF 25...

Embodiment 2

[0026] Wash 2kg of fresh ginger, cut into thin slices, and dry below 60°C. Pulverize in a pulverizer, pass through a 100-mesh sieve, and put it into a 1000mL Erlenmeyer flask. According to the ratio of ginger powder: acetone volume ratio of 1:2-3, add acetone, airtight soak for 24 hours, shake once every 4 hours during soaking; repeat soaking 3 times, combine extracts, and recover under reduced pressure below 40°C solvent to obtain 80 g of extract extract.

[0027]Dissolve the above extract with 200mL of ethyl acetate, add 250g of 200-300 mesh silica gel for column chromatography to mix the sample, heat in a water bath to evaporate the ethyl acetate, then add 2kg of blank silica gel (200-300 10BV of petroleum ether (60-90°C) for eluting, then 8BV of petroleum ether: ethyl acetate with a volume ratio of 20:1, and finally with petroleum ether: ethyl acetate with a volume ratio of 10 : 1 elution, TLC detection elution progress. The relatively pure 8-gingerol and 6-gingerol com...

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Abstract

The invention relates to a method for preparing 6- gingerol and 8- gingerol with fresh ginger. It is characterized in that it compries following steps: cutting fresh ginger into sheets, drying at lowtemperature, disintegrating, sifting with screen of 100 order, heating and refluxing and extracting with acetic ester or acetone, or extracting through immersion, extracting for three times, combiningextract liquid, decompressing and recovering solvent, getting extract concretem dissloving it with acetic ester, treating it with chromatographic silica gel and washing it with benzinum purificatum acetic ester with gradient solvent, checking washing process by using TLC, decompressing to recover solvent and getting coarse 6- gingerol and 8- gingerol; washing coarse product with chloroform- carbinol solvent in Sephadex LH20 gel column, checking washing process with TLC, and getting washung liquid containing 6- gingerol and 8- gingerol with higher purity, decompressing to recover solvent and getting pure 6- gingerol and 8- gingerol, checking content with HPLC. The invention is characterized by simple operation, high productivity and production of two kinds of product at the same time.

Description

technical field [0001] The invention relates to a method for preparing 6-gingerol and 8-gingerol from ginger, in particular to a method for extracting, separating and purifying 6-gingerol and 8-gingerol from ginger, which belongs to natural active products Preparation method technical field. Background technique [0002] Ginger is a common food that is indispensable in daily life, and it is also an important traditional Chinese medicine in traditional Chinese medicine. Medicinal ginger is the fresh rhizome of ginger, that is, fresh ginger, which is pungent in taste and slightly warm in nature, and belongs to the lung, spleen and stomach meridians. Dispelling the exterior and dispelling cold, warming the middle to stop vomiting, resolving phlegm and relieving cough. Indications for anemofrigid cold, stomach cold vomiting, cough due to cold phlegm. Modern studies have found that ginger has many biological activities such as improving the activity of digestive enzymes, prote...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/255C07C45/78
Inventor 宣伟东卞俊陈海飞王朝武袁兵鲍蕾蕾成熙周静静
Owner NO 411 HOSPITAL OF PLA
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