Riemerella anatipestifer antibody indirect ELISA method detection kit and application thereof

Abstract

The invention relates to a riemerella anatipestifer antibody indirect ELISA method detection kit and an application thereof; the kit comprises: a) an antibody detection plate, b) enzyme conjugate working fluid, c) a positive control, d) a negative control, e) a sample diluent, f) 10X concentrated washing liquid, g) a substrate coloured solution A, h) a substrate coloured solution B, and i) a stopping solution; the beneficial and positive effects of the invention are that: the kit is simple in operation; the requirements for apparatuses needed are not high; the kit can be operated by everyone, can meet the requirements with different levels such as epidemic disease monitoring, hygienic epidemic prevention, intensive culture, and individual culture, is suitable for large-scope popularization and application, and has wide market prospects.

Description

technical field [0001] The invention relates to a rapid diagnosis method and apparatus for bacterial antibodies of ducks, in particular to an indirect ELISA detection kit for Riemerella anatipestifer antibodies and its application. Background technique [0002] Riemerella anatipestifer infection (RAI) is a contagious disease of domestic ducks, turkeys, and a variety of other birds. The disease is currently distributed worldwide and has been found in all countries with intensive duck production. The disease mainly affects ducklings, goslings and young turkeys aged 1 to 8 weeks (especially 2 to 8 weeks). The disease often presents an acute or chronic septic process, mainly characterized by neurological symptoms and fibrinous pericarditis, perihepatitis, and air sacculitis. The sickness rate of this disease can reach more than 9%, and the lethal rate can be as high as more than 75%. The disease-resistant duck often grows into a defective duck or a stiff duck, and the feed co...

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Problems solved by technology

[0003] The serotype of Riemerella anatipestifer is complex, currently recognized as having 21 serotypes, and the development of serotypes in my country is becoming more and more complicated. Drug prevention is one of the main measures to control the disease in my country, but many research reports have shown that duck plague Riemerella has developed resistance to most commonly used drugs in production. At the same time, the large-scale use of drugs has also caused a great threat to food safety. Immunization will gradually replace drug prevention as one of the safe and effective measures to control the disease

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