Rhodamine B ELISA detection method

An immunoassay method, B enzyme technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of many pretreatment steps, time-consuming detection costs, unsuitable samples, etc., and achieve low cost, good stability and repeatability Effect

Inactive Publication Date: 2010-10-27
JIANGNAN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] Rhodamine B residue analysis methods mainly include high-performance liquid chromatography (HPLC). Although these methods have high sensitivity, there are many pre-treatment steps for samples, which are relatively time-consuming and expensive to detect, especially not suitable for screening a large number of samples.

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  • Rhodamine B ELISA detection method
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  • Rhodamine B ELISA detection method

Examples

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Embodiment Construction

[0059] The present invention is further illustrated by the following examples.

[0060] 1. Instrument:

[0061] TGL-40B Desktop Low Speed ​​Centrifuge Shanghai Anting Scientific Instrument Factory

[0062] KFLOW water purifier Keffron Corporation

[0063] ZD-9556 Horizontal Shaker Taicang Science and Education Equipment Factory

[0064] Costar 96-well 8×12 detachable microtiter plate Shanghai Jitai Biotechnology Co., Ltd.

[0065] MuLtiska Mks Microplate Reader Thermo Labsystems

[0066] Adjustable Pipettes Thermo Labsystems

[0067] Vortex mixer Shanghai Huxi Instrument Analysis

[0068] 2. Reagents:

[0069] Horseradish peroxidase-labeled goat anti-rabbit IgG (HRP-IgG) Kangcheng Bioengineering Co., Ltd.

[0070] Tetramethylbenzidine (TMB) Huamei Bioengineering Company

[0071] Other reagents are analytical reagents

[0072] 3. Steps

[0073] 1. Synthesis of immunogen and coater

[0074] The immunogen was synthesized by mixed anhydride method (rhodamine B hapten co...

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Abstract

The invention relates to a rhodamine B ELISA detection method, which belongs to the technical field of ELISA. The invention discloses a rhodamine B ELISA detection method, which comprises the following steps: using synthesized rhodamine B immunogen for immunizing healthy New Zealand rabbits to obtain polyclonal antibodies; and using rhodamine B as standard products; using rhodamine B hapten and OVA coupling materials as coating antigen to establish a rhodamine B indirect ELISA method. The invention provides the fast and efficient detection method for the residual detection on the rhodamine B. Because the polyclonal antibodies are adopted, the cost is low, and in addition, the stability and the repetitiveness are good. The detectability (IC90) is 0.028 ng/mL, the half inhibitory amount (IC50) is 1.3 ng/mL, and the detection range (IC20 to IC80) is between 0.07 and 17.5 ng/mL. Because of high specificity and high compatibility of the immune reaction, high selectivity and high sensitivity can be obtained when the ELISA is used for detecting the rhodamine B.

Description

technical field [0001] An enzyme-linked immunosorbent detection method for rhodamine B relates to a detection method for pigment residues, more specifically, an enzyme-linked immunosorbent assay (ELISA) is used to detect the residues of rhodamine B. The invention belongs to the technical field of enzyme-linked immunoassay (ELISA). Background technique [0002] Rhodamine B (Rhodamine B) is a triphenylmethane basic dye with potential carcinogenicity and mutagenicity. It is not allowed to be used in food in my country and the European Union. It is a kind of artificially synthesized dye with bright pink color, which has strong fluorescence in the solution. It is used as cell fluorescence staining agent in the laboratory, colored glass, special fireworks and firecrackers and other industries. It is not allowed to be used for food dyeing. Rhodamine B is fat-soluble and is illegally used as a coloring agent in condiments (mainly chili powder and chili oil). Contaminated condiment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/531
Inventor 胥传来宋珊珊林菲赵媛赵书阁刘微波
Owner JIANGNAN UNIV
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