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Permithrin polyclonal antibody enzyme linked immunity detecting method

An enzyme-linked immunoassay and polyclonal antibody technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex preparation process of monoclonal antibody, lack of trace analysis sensitivity, unsuitable for popularization, etc., and achieve high selectivity And the effect of good sensitivity, stability and repeatability, and low cost

Inactive Publication Date: 2005-06-01
NANJING UNIV
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Problems solved by technology

However, the above method requires that the environmental sample must be purified or concentrated, otherwise it does not have the sensitivity of trace analysis
Moreover, monoclonal antibodies are mostly used. The preparation process of monoclonal antibodies is complicated and expensive, so it is not suitable for popularization.
There is still a lack of practical methods for the detection of permethrin residues in soil matrices

Method used

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  • Permithrin polyclonal antibody enzyme linked immunity detecting method
  • Permithrin polyclonal antibody enzyme linked immunity detecting method
  • Permithrin polyclonal antibody enzyme linked immunity detecting method

Examples

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Embodiment Construction

[0039] Further illustrate the present invention below by embodiment

[0040] 1. Instrument:

[0041] Microplate reader (MK3, Labsystem, Finland)

[0042] Dual-wavelength UV-Vis spectrophotometer (Shimadzu, UV-190, Japan)

[0043] Freeze dryer (Labconco, 4.5L, USA)

[0044] High-speed refrigerated centrifuge (HITACHI, Japan)

[0045] Magnetic stirrer (Werke, IKA, Germany)

[0046] Vortex mixer (MS2, IKA, Germany)

[0047] Micro shaker (KSI30, IKA, Germany)

[0048] 2. Reagents:

[0049] Horseradish peroxidase (Shanghai Xueman Biotechnology Co., Ltd., BR)

[0050] 1-Ethyl-3, (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC.HCl) (Shanghai Livzon Dongfeng Biological Preparations Co., Ltd., BR)

[0051] N-hydroxysuccinimide (China Pharmaceutical Group Shanghai Chemical Reagent Company, BR)

[0052] 1,4 dioxane (Shanghai Lingfeng Chemical Reagent Co., Ltd., CR)

[0053] DMF (Shanghai No.1 Reagent Factory, CR)

[0054] Enzyme-labeled goat anti-rabbit GAR-HRP (Huamei...

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Abstract

The present invention discloses one kind of permethrine polyclonal antibody enzyme-linked immunoassay method. Synthetic permethrine immune antigen is utilized in immunizing health rabbit to obtain polyclonal antibody, and after screening, the indirect competitive enzyme-linked immunoassay method for detecting residual permethrine in soil and food is established with permethrine as standard article and permethrine semi-antigen and OVA conjugate as coating antigen. The method is fast and efficient, and has relatively low cost, high stability and high repeatability, sensitivity of 0.1 ppb, linear range of 10-800 ppb, high selectivity and simple sample pre-treatment.

Description

1. Technical field [0001] The invention relates to a method for detecting pesticide residues, more specifically detecting the residues of permethrin in soil and food by enzyme-linked immunosorbent assay (ELISA). 2. Background technology [0002] With the gradual withdrawal of the once dominant organophosphorus pesticides from the market, pyrethroid pesticides have become more and more widely used. Wherein permethrin accounts for more than 53%. It is generally believed that pyrethroid pesticides are safe to mammals, but permethrin is highly toxic to site-free invertebrates and fish and has a high bioaccumulation concentration in some aquatic animals . Contaminated water and aquatic food are consumed by people, which is potentially dangerous. It is reported that the human body will experience immune system toxicity and suppressive reversible symptoms after exposure to permethrin. Some permethrin pesticides can also cause lymph node and spleen damage and even cancer. Proper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/543
Inventor 孙成刘廷凤塔娜邹惠仙赵晓联张莲芬杨敏娜刘亚子周芳钟明
Owner NANJING UNIV
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