ELISA detection method for penicillins antibiotic residue

An enzyme-linked immunoassay detection and antibiotic technology, which is applied in the field of immunoassay to achieve fast and efficient detection methods, good stability and repeatability, and low cost.

Inactive Publication Date: 2009-04-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing microbial detection methods and instrumental detection methods are difficult to achieve rapid, accurate and quantitative detection of all penicillin antibiotics
There are many types of penicillin antibiotics. So far, it is difficult to quickly and accurately detect all penicillin antibiotics with existing microbial detection methods and instrumental detection methods. It is necessary to study rapid and sensitive immunoassay techniques

Method used

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  • ELISA detection method for penicillins antibiotic residue

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0046] specific implementation plan

[0047] The present invention is further illustrated by the following examples.

[0048] 1. Instrument:

[0049] TGL-40B desktop low-speed centrifuge Shanghai Anting Scientific Instrument Factory;

[0050] KFLO pure water machine Furlong company;

[0051] ZD-9556 horizontal shaking table Taicang Science and Education Equipment Factory;

[0052] Costar 96-well 8×12 detachable enzyme plate Shanghai Jitai Biotechnology Co., Ltd.;

[0053] MuLtiska Mks microplate reader Thermo Labsystems;

[0054] Adjustable pipette Thermo Labsystems;

[0055] FA25 Homogenizer Fluka;

[0056] Vortex mixer Shanghai Huxi Instrument Analysis Factory.

[0057] 2. Reagents:

[0058]Horseradish peroxidase-labeled goat anti-rabbit GAR-HRP Kangcheng Bioengineering Company;

[0059] Tetramethylbenzidine (TMB) Huamei Bioengineering Company;

[0060] All other reagents were of analytical grade.

[0061] 3. Steps

[0062] 1. Synthesis of Immunogen and Coatogen ...

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Abstract

The invention provides a multi-residual enzyme-linked immunoassay method used for antibiotics such as penicillins and the like, belonging to the technical field of immunoassay. The method obtains a polyclonal antibody by utilizing immunogen immunity synthesized by 6-amino penicillanic acid; the antibiotics such as the penicillins and the like are used as standard products and the conjugate of the 6-amino penicillanic acid and OVA is used as coating antigen, thus establishing the indirect ELISA method of antibiotics such as penicillins and the like in animal foods. The method provides a quick and high-efficiency detection means for the residual detection of the antibiotics such as penicillins and the like in the animal foods; as the polyclonal antibody is adopted, the expense is lower and the stability and repeatability are better; furthermore, the method has the advantages of simple predisposal, high sensitiveness, high precision and the like.

Description

technical field [0001] The invention relates to a method for detecting antibiotic residues, more specifically detecting multiple residues of penicillin antibiotics in animal foods by using an enzyme-linked immunosorbent assay (ELISA), and belongs to the technical field of immunodetection. Background technique [0002] Penicillin antibiotics are β-lactam antibiotics, which refer to a class of antibiotics containing a β-lactam ring in their chemical structure. , showing bactericidal activity. In animal husbandry, penicillin antibiotics are widely used to control mastitis in dairy cows, and to treat animal urinary tract, gastrointestinal tract and respiratory tract infections. However, due to its improper use, non-compliance with the withdrawal period regulations, illegal and excessive addition, etc., it can cause its residue in animal products and cause serious harm to human health. These antibiotics are widely used in the prevention and treatment of human and animal disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 胥传来马伟彭池方徐丽广李灼坤
Owner JIANGNAN UNIV
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