7 results about "Penicillin Antibiotic" patented technology

This invention discloses a penicillinase mutant, its recombinant strain, and its applications, relating to the field of antibiotic wastewater treatment. Using the wild-type penicillinase of SEQ ID NO:1 as a template, this invention obtains penicillinase mutants of SEQ ID NO:2-7 through site-directed combination mutagenesis, significantly improving wide pH tolerance. While the wild-type enzyme experiences a sharp drop in activity at pH 9-10, the mutant of this invention maintains high enzyme activity at pH 5-10, reaching over 90% of the highest wild-type activity at pH 9-10. It can be directly adapted to the highly alkaline environment of penicillin G production wastewater without significant pH adjustment. This invention also provides the recombinant genetic material encoding the mutant, the recombinant strain, and its application in wastewater purification, exhibiting advantages such as high degradation efficiency, low operating costs, stable process, and environmental friendliness, possessing significant engineering application value in the treatment of penicillin antibiotic wastewater.

The invention discloses a method for promoting the degradation of penicillin antibiotics in water, comprising: S1, water quality detection; S2, medium adjustment; S3, catalytic degradation; S4, precipitation reaction. The invention uses calcium ions and organic matter to adjust the medium of the water to be treated, so that the water to be treated can be in a good state. The calcium ions can coordinate with the carbonyl and carboxyl groups in the penicillin molecules, stabilize the hydrolysis reaction intermediates, and accelerate the hydrolysis rate of the penicillin antibiotics. The characteristic functional groups such as amino groups and thiol groups in the biological metabolic organic matter can also serve as strong nucleophilic reagents to promote the rapid hydrolysis of the penicillin antibiotics. At the same time, through the specific setting of the method, it is possible to avoid secondary pollution of the water to be treated by calcium ions and biological organic metabolic media before degradation.

The invention relates to the technical field of microbial culture, in particular to a klebsiella pneumoniae chromogenic medium as well as a preparation method and application thereof. The klebsiella pneumoniae chromogenic medium is prepared from the following raw materials: a core nutrient component, a composite salt system, a synergistic compound chromogenic system, a targeted composite antibacterial agent, a function enhancer, agar and deionized water, the synergistic compound color developing system comprises a composite color developing agent and a color developing synergist, wherein the composite color developing agent is prepared from 5-bromo-4-chloro-3-indole-beta-D-galactoside, indole acetate and naphthol-AS-D-acetate according to the weight ratio of (3.5 to 4.5) to (2.5 to 3.5) to (2.5 to 3.5); the targeted composite antibacterial agent comprises penicillin antibiotics and erythromycin in a weight ratio of (5-8): (2-4). The comprehensive advantages of high separation positive rate, comprehensive infectious microbe inhibition, high color development specificity, outstanding anti-interference capability and excellent stability are realized.

The present invention discloses a pharmaceutical composition for preventing and treating mastitis in dairy cows and a preparation method thereof, relating to the field of veterinary medicine. The pharmaceutical composition is composed of the following components in weight percentage: 1-10% penicillin antibiotics, 0.5-5% beta-lactamase inhibitors, 0.1-2% corticosteroids, 0.5-10% oily solvents, 1-30% emulsifiers, 0.5-10% stabilizers, 0.1-2% antioxidants, and water. The present invention utilizes the above components to construct a multiphase homogeneous nanoemulsion, effectively improving the stability and bioavailability of the pharmaceutical composition.

To provide a novel antimicrobial product that enables the management of animal diseases while reducing reliance on conventional antibiotics. [Solution] The present invention relates to an antimicrobial composition comprising an extract of a coffee composition and an antibiotic, and to the use of the antimicrobial composition in the manufacture of antimicrobial drugs. The extract of the coffee composition comprises a solid component and water, the solid component comprising coffee beans and auxiliary materials. The antibiotic is selected from the group consisting of penicillin antibiotics, cephalosporin antibiotics, fluoroquinolone antibiotics, tetracycline antibiotics, chloramphenicol antibiotics, aminoglycoside antibiotics and combinations thereof, and the weight ratio of the coffee composition to the antibiotic is from 1.5:1 to 25000:1. The antimicrobial composition of the present invention can exhibit a superior antibacterial effect compared to the use of antibiotics alone, and this antibacterial effect can also be exerted against drug-resistant bacteria.

The invention provides a Delftia sp. PG-8, application thereof and a method for degrading penicillin G potassium. The preservation number of the Delftia sp. Is CCTCC (China Center For Type Culture Collection) NO: M 2024394, and the Delftia sp. Is named as Delftia sp. In taxonomy; the Delftia sp. PG-8 disclosed by the invention has high-concentration resistance to penicillin, can grow by taking penicillin G potassium as a unique carbon source, and can completely degrade the penicillin G potassium under the conditions that the pH is 5-9 and the temperature is 25-40 DEG C; the strain is helpful for efficient degradation of penicillin antibiotics in the environment, and can also provide degradation gene resources for penicillin pollutants in the bioengineering treatment environment. The method has a very strong application prospect in treatment of penicillin pollutants such as restoration of environment polluted by beta-lactamase antibiotics, green biodegradation of mushroom dregs generated in the process of fermentation production of the beta-lactamase antibiotics and the like.

The invention relates to the synthesis of novel penicillin β-lactam antibiotics with synthetic peptide structures containing C-C and C-N multiple bonds, and the synthesis of their pharmaceutically acceptable salts, via four-component reactions using 6-aminopenicillanic acid (6-APA) as the aldehyde, amine, isonitrile, and carboxylic acid, or 6-aminopenicillanic acid (6-APA) as the aldehyde carboxylic acid and amine.