46results about How to "Enhance humoral immune response" patented technology

The present invention discloses an application of ginseng total saponin or monomer saponin Rb1 as vaccine immunologic adjuvant. The ginseng total saponin or monomer saponin Rb1 and aluminium hydroxide are mixed and used as vaccine immunologic adjuvant, and as compared with traditional aluminium hydroxide adjuvant said invention possesses the following advantages: (1) its side effect is less; (2) said vaccine can be stored under the condition of frozen low-temp. and it can prolong effective period of vaccine; (3) its method is simple and convenient, its quality is easy to be controlled; and (4) it can induce body to produce higher humoral immunity response.

Provided are an Ebola virus envelope glycoprotein (that is GP protein) codon optimized nucleotide sequence, a human replication deficient recombinant adenovirus capable of expressing the nucleotide sequence, and applications in preparing a vaccine for preventing Ebola virus diseases. The nucleotide sequence takes a replication deficient 5 type adenovirus that is lack of E1 and E3 in a combined mode as a vector. HEK293 cells that integrate adenovirus E1 genes serve as a packaging cell line, and carried protective antigenic genes are codon optimized Zaire type Ebola virus Makona strain envelope glycoprotein genes. After the envelope glycoprotein genes are optimized by codon, the expression level in transfection cells is obviously improved.

The invention relates to a composite adjuvant, a vaccine comprising the same and a preparation method of the vaccine. The composite adjuvant comprises a noncompetitive inhibitor of indoleamine-2,3 dioxygenase and aluminum salts, the noncompetitive inhibitor of the indoleamine-2,3 dioxygenase is 4-benzimidazole, the content of the 4-benzimidazole is 0.5-1.5 mg, the aluminum salts are aluminium hydroxide, and the content of the aluminium hydroxide is 300 mu g. The invention further relates to the vaccine comprising the composite adjuvant and a preparation method of vaccine. The raw materials of the composite adjuvant are easy to obtain, a preparation process of the vaccine is simple, the cost is low, the performance is stable, toxic and side effects are small, immune response is promoted jointly from the positive aspect and the negative aspect of organism immunoregulation, the composite adjuvant is safe and reliable when used in a range of immunizing dose, humoral immune response of antigenic specificity can be induced effectively, humoral immune responses of the antigenic specificity are enhanced remarkably, and the effect of the composite adjuvant is higher than the effects of a group without adjuvant, a group with a single adjuvant and a group with an aluminum adjuvant.

The invention discloses a respiratory syncytial virus vaccine, and a preparation method and an application thereof. The active components of the respiratory syncytial virus vaccine comprise a respiratory syncytial virus G protein and an immunosuppressant; the amino acid sequence of the respiratory syncytial virus G protein is represented by sequence 2 in a sequence table; and the immunosuppressant is cyclosporins A. Test proves that the respiratory syncytial virus vaccine can enhance the humoral immunoreaction in the virus neutralizing antibody level of immune animals, can inhibit too strong cellular immunity reactions, effectively and specifically inhibits inflammation related pathological reactions, and has the advantages of manure technology, low cost, no side effects and easy promotion.

The present invention relates to the mannose-receptor selective lysinylated cationic amphiphile and a process for preparation thereof. The compounds of the present invention can target DNA vaccines to antigen presenting cells (APCs) such as macrophages and dendritic cells (DCs), via mannose receptors expressed on the cell surface of APCs. The cationic amphiphiles disclosed herein show enhanced cellular and humoral immune response compared to their mannosyl counterparts in genetic immunization in mice. The present invention discloses that immunization with electrostatic complexes (lipoplexes) of DNA vaccines encoding melanoma antigens (gp100 and tyrosinase) and liposome of the presently described novel lysinylated cationic amphiphiles with mannose-mimicking shikimoyl head-groups provides long-lasting (100 days post melanoma tumor challenge) protective immunity in all immunized mice. Cationic amphiphiles with mannose-mimicking shikimoyl head-groups described in the present invention are likely to find future applications in the field of genetic immunization.

An altered avian NDV that contains the coding sequence of avian interleukin-4 (IL-4), or a portion thereof, in the reverse orientation suppresses in-ovo IL-4 production via RNAi when administered to embryonic birds. An immunogenic composition containing this altered NDV is included in this invention. The altered avian NDV can, optionally contain a polynucleotide encoding a heterologous antigen from a heterologous avian pathogen and can produce said heterologous antigen in-ovo.