Preparation method and application of chitosan-Pickering emulsion interleukin 12 adjuvant system

A technology of chitosan and chitosan water, applied in the preparation of chitosan-Pickering emulsion interleukin 12 adjuvant system and its application field, can solve the problem of immune dose, method, mechanism of immune enhancement, unexplained, and active agent Safety and stability hidden dangers and other issues, to achieve the effect of excellent humoral immunity and cellular immunity, and enhanced immune level

Active Publication Date: 2022-01-18
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also problems such as the use of active agents that bring safety and stability risks, and the immune dose, method, and specific immune enhancement mechanism have not yet been clarified

Method used

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  • Preparation method and application of chitosan-Pickering emulsion interleukin 12 adjuvant system
  • Preparation method and application of chitosan-Pickering emulsion interleukin 12 adjuvant system
  • Preparation method and application of chitosan-Pickering emulsion interleukin 12 adjuvant system

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Embodiment 1

[0045] The present inventor provides a kind of chitosan-Pickering emulsion / IL-12 adjuvant system and Chlamydia trachomatis recombinant protein to be prepared as vaccine preparation, confirmed through animal experiment that this vaccine preparation can enhance the immune level of animal body anti-Chlamydia trachomatis infection, And the inventors found that the chitosan-Pickering emulsion / IL-12 adjuvant system enhanced the humoral immunity and cellular immunity of the antigen better than no adjuvant or adjuvant alone. Each group of reagents and consumables used in the present invention can be purchased on the market. On this basis, the inventor has completed the present invention, specifically:

[0046] 1. Preparation of Chitosan-Pickering Emulsion

[0047] Dissolve dry powder chitosan in water, add appropriate amount of acetic acid, shake in an electromagnetic stirrer at 220rpm / min overnight, then filter with G3 sand core funnel, get chitosan solution after removing impurities...

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Abstract

The invention relates to the field of immunology, in particular to a preparation method and application of a chitosan-Pickering emulsion interleukin 12 adjuvant system. Mixing chitosan with water to obtain a water phase; and mixing the water phase with oil phase liquid paraffin, and carrying out ultrasonic treatment to obtain the chitosan-Pickering emulsion. Combining a chitosan-Pickering emulsion with IL-12 to form an adjuvant, and then forming a vaccine with the adjuvant and pORF5. The invention provides a vaccine preparation prepared from a chitosan-Pickering emulsion/IL-12 adjuvant system and chlamydia trachomatis recombinant protein. Experiments prove that the chitosan-Pickering emulsion/IL-12 adjuvant system enhances the humoral immunity and cellular immunity level of an antigen better than those of an antigen without an adjuvant or containing a single adjuvant.

Description

technical field [0001] The present invention relates to the field of immunology, in particular to the application of Pickering emulsion interleukin 12 adjuvant system in the preparation of Chlamydia trachomatis vaccine, that is, a Chlamydia trachomatis recombinant subunit vaccine is preferably a chitosan-Pickering emulsion / IL-12 adjuvant Chlamydia trachomatis vaccine preparation prepared by mixing dose systems, its preparation method, and its application in the prevention and treatment of Chlamydia trachomatis infection. Background technique [0002] Chlamydia trachomatis (Ct), as a Gram-negative obligate intracellular parasite, is one of the most common sexually transmitted pathogens in the world. It can infect human eyes, respiratory tract and reproductive tract system, causing eye scars and blindness. , Male urethritis, proctitis, female cervicitis, pelvic inflammatory disease, ectopic pregnancy and even infertility and other diseases, seriously endangering human health. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/118A61P31/04A61P37/04
CPCA61K39/118A61K39/39A61P31/04A61P37/04A61K2039/55583A61K2039/55566A61K2039/55538
Inventor 李忠玉舒明艺赵兰华文雅婷
Owner NANHUA UNIV
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